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The map label for this gene is insA [H]

Identifier: 30064399

GI number: 30064399

Start: 3170300

End: 3170599

Strand: Direct

Name: insA [H]

Synonym: S3295

Alternate gene names: 30064399

Gene position: 3170300-3170599 (Clockwise)

Preceding gene: 30064397

Following gene: 30064403

Centisome position: 68.93

GC content: 54.0

Gene sequence:

>300_bases
GTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACA
TCAGCGCTATCTCTGCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACC
AGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTT
TTACGTCACTTAAAAACTCAGGCCGCAGTCGGTAACCTCGCGCATACAACCGGGCAGTGA

Upstream 100 bases:

>100_bases
TATTCCAAGGTAAATATTGCGTCACTAAATGGACATTGGAGTGACGGTAATGCTGCCAACTTACTGATTTAGTGTATGAT
GGTGATTTTAAGGTGCTTGC

Downstream 100 bases:

>100_bases
TGTGATTGTCTGCGCTGAAATGGACGAACATTGGGGCTACGTCGGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCG
TATGACAGGATACGGAGGAC

Product: IS1 orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 99; Mature: 98

Protein sequence:

>99_residues
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTV
LRHLKTQAAVGNLAHTTGQ

Sequences:

>Translated_99_residues
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTV
LRHLKTQAAVGNLAHTTGQ
>Mature_98_residues
ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVL
RHLKTQAAVGNLAHTTGQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1790747, Length=85, Percent_Identity=97.6470588235294, Blast_Score=174, Evalue=1e-45,
Organism=Escherichia coli, GI1789852, Length=85, Percent_Identity=94.1176470588235, Blast_Score=173, Evalue=2e-45,
Organism=Escherichia coli, GI1788204, Length=85, Percent_Identity=94.1176470588235, Blast_Score=173, Evalue=2e-45,
Organism=Escherichia coli, GI1786204, Length=85, Percent_Identity=94.1176470588235, Blast_Score=173, Evalue=2e-45,
Organism=Escherichia coli, GI87081814, Length=85, Percent_Identity=92.9411764705882, Blast_Score=172, Evalue=4e-45,
Organism=Escherichia coli, GI1786471, Length=85, Percent_Identity=92.9411764705882, Blast_Score=172, Evalue=4e-45,
Organism=Escherichia coli, GI1786460, Length=85, Percent_Identity=92.9411764705882, Blast_Score=172, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012287
- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10630; Mature: 10499

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMA
CCCEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHH
MNGVGCRASARIMGVGLNTVLRHLKTQAAVGNLAHTTGQ
HCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMA
CCEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHH
MNGVGCRASARIMGVGLNTVLRHLKTQAAVGNLAHTTGQ
HCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]