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The map label for this gene is insG [H]

Identifier: 30064397

GI number: 30064397

Start: 3169116

End: 3169391

Strand: Direct

Name: insG [H]

Synonym: S3292

Alternate gene names: 30064397

Gene position: 3169116-3169391 (Clockwise)

Preceding gene: 30064396

Following gene: 30064399

Centisome position: 68.9

GC content: 49.28

Gene sequence:

>276_bases
ATTACCAGATATCAGATGATTAAAATGGCGGAACATCTGAAAGGTTACTGGCCGAATCAACTGAGTTTCTCAGAATCATG
CGGAATGGTGATGAGAATGCTGATGACATTGCAGGGCGCTTCACCGGGACGTATACCGGAGCTGATGCGCGATCTTGCAA
GTATGGGACAACTTGTGAAATTACCGACGAGAAGGGGAAGGGCCTTCCCGAGAGTGGTAAAGGAGAGGCCCTGGAAATAC
CCCACAGCCCCGAAAAAGAGCCAGTCAGTTGCTTAA

Upstream 100 bases:

>100_bases
GCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAAC
ACTATCAGTAAGTTGGAGTC

Downstream 100 bases:

>100_bases
CTGACTGGCATTACACCGTTAGTGAGACGTTTAGTGTAGTCATGACAAAAGATCCTGGTGCGGCCTGTACTGAGCAATTC
AATATTTTAGGGAGCTTTTT

Product: IS4 orf

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 90

Protein sequence:

>91_residues
MTRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKY
PTAPKKSQSVA

Sequences:

>Translated_91_residues
MTRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKY
PTAPKKSQSVA
>Mature_90_residues
TRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYP
TAPKKSQSVA

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=91, Percent_Identity=96.7032967032967, Blast_Score=184, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 10472; Mature: 10340

Theoretical pI: Translated: 11.37; Mature: 11.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
9.9 %Met     (Translated Protein)
11.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
8.9 %Met     (Mature Protein)
10.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVK
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LPTRRGRAFPRVVKERPWKYPTAPKKSQSVA
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVK
CHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LPTRRGRAFPRVVKERPWKYPTAPKKSQSVA
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]