Click here to switch to the map view.

The map label for this gene is bioF [H]

Identifier: 30064333

GI number: 30064333

Start: 3110642

End: 3111430

Strand: Direct

Name: bioF [H]

Synonym: S3226

Alternate gene names: 30064333

Gene position: 3110642-3111430 (Clockwise)

Preceding gene: 30064332

Following gene: 30064341

Centisome position: 67.63

GC content: 52.72

Gene sequence:

>789_bases
GTGGTGTTACTGGATGCCGACAGCCACGCCAGTATTTATGACGCCTGTTCTCTCGGTGGCGCGGAAATTATTCGTTTCCG
CCATAACGATGCTAAAGATCTGGAACGGCGCATGGTTCGCCTCGGTGAACGGGCGAAAGAGGCGATTATCATCGTTGAGG
GCATCTACAGCATGCTCGGTGACGTTGCGCCGCTGGCGGAAATTGTTGATATCAAACGTCGTCTCGGTGGTTATTTGATT
GTCGATGAAGCGCATTCGTTTGGCGTGTTAGGCGCAACGGGGCGCGGACTGGCTGAAGCTGTCGGCGTTGAAGATGATGT
GGATATTATCGTTGGTACTTTCAGTAAAAGTCTGGCCTCGATTGGCGGTTTTGCTGTCGTTTCGGAAGCGATGGAAGTGC
TGCGCTACGGCAGCCGCCCGTATATTTTTACCGCCTCTCCTTCTCCGTCCTGCATTGCGACCGTGCGCTCGTCGTTAAGA
ACTATTGCCACACAACCAGAGCTGCGGCAAAAGCTGATGGATAACGCCAACCATCTGTATGACGGTCTGCAAAAACTGGG
TTATGAGTTAAGTTCACATATTAGCCCGGTAGTGCCAGTAATAATCGGTTCGAAAGAGGAAGGTTTACGCATCTGGCGCG
AGCTTATCTCGCCGGGCGTGTATGTGAATCTTATCCTGCCGCCTGCCGCGCCTGCAGGCATCACGCTGCTGCGCTGTAGC
GTAAATGCTGCCCACAGTCGTGAGCAAATCGATGCCATCATTCAGGCATTTGCTACTCTGAAGCAGTAA

Upstream 100 bases:

>100_bases
AAGAGATTGCCGAGTTTTTCAATCGCCCTACCGCTATCGTTTTTCAACCGGTTATACCGCTAATTTAGGCGTCATCAGCG
CACTTGCCGACCATAACGCC

Downstream 100 bases:

>100_bases
TGTGAGCCCGCCGTGGTGTTTGCGCCACGGCGGGTTTAATGTTGTCTGTCATACCAATACACCAGGCTCAACGCCACCAA
CACAAACGCTACCGGTAAAC

Product: putative class II aminotransferase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MVLLDADSHASIYDACSLGGAEIIRFRHNDAKDLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLI
VDEAHSFGVLGATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVVSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLR
TIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKEEGLRIWRELISPGVYVNLILPPAAPAGITLLRCS
VNAAHSREQIDAIIQAFATLKQ

Sequences:

>Translated_262_residues
MVLLDADSHASIYDACSLGGAEIIRFRHNDAKDLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLI
VDEAHSFGVLGATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVVSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLR
TIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKEEGLRIWRELISPGVYVNLILPPAAPAGITLLRCS
VNAAHSREQIDAIIQAFATLKQ
>Mature_262_residues
MVLLDADSHASIYDACSLGGAEIIRFRHNDAKDLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLI
VDEAHSFGVLGATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVVSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLR
TIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKEEGLRIWRELISPGVYVNLILPPAAPAGITLLRCS
VNAAHSREQIDAIIQAFATLKQ

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=258, Percent_Identity=31.3953488372093, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI284448556, Length=258, Percent_Identity=31.3953488372093, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI4758668, Length=272, Percent_Identity=31.9852941176471, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI119220554, Length=273, Percent_Identity=31.5018315018315, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI83977440, Length=255, Percent_Identity=28.6274509803922, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI83977442, Length=255, Percent_Identity=28.6274509803922, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI83977444, Length=255, Percent_Identity=28.6274509803922, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI4502025, Length=263, Percent_Identity=28.8973384030418, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI40316939, Length=263, Percent_Identity=28.8973384030418, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI5454084, Length=237, Percent_Identity=29.535864978903, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1790046, Length=259, Percent_Identity=34.3629343629344, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1786993, Length=254, Percent_Identity=32.6771653543307, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994529, Length=263, Percent_Identity=33.4600760456274, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32566772, Length=273, Percent_Identity=32.6007326007326, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17560912, Length=273, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=277, Percent_Identity=30.6859205776173, Blast_Score=127, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71982617, Length=202, Percent_Identity=30.6930693069307, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982625, Length=202, Percent_Identity=30.6930693069307, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320267, Length=272, Percent_Identity=30.8823529411765, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320438, Length=280, Percent_Identity=26.4285714285714, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323954, Length=198, Percent_Identity=34.8484848484849, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24662918, Length=258, Percent_Identity=34.1085271317829, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI17136286, Length=274, Percent_Identity=32.4817518248175, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17137420, Length=257, Percent_Identity=28.0155642023346, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653280, Length=197, Percent_Identity=28.4263959390863, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653276, Length=197, Percent_Identity=28.4263959390863, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653278, Length=197, Percent_Identity=28.4263959390863, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 28281; Mature: 28281

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLDADSHASIYDACSLGGAEIIRFRHNDAKDLERRMVRLGERAKEAIIIVEGIYSMLG
CEEECCCCCCHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHH
DVAPLAEIVDIKRRLGGYLIVDEAHSFGVLGATGRGLAEAVGVEDDVDIIVGTFSKSLAS
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCCHHEEHHHHHHHHH
IGGFAVVSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLRTIATQPELRQKLMDNANHLY
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHH
DGLQKLGYELSSHISPVVPVIIGSKEEGLRIWRELISPGVYVNLILPPAAPAGITLLRCS
HHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEC
VNAAHSREQIDAIIQAFATLKQ
CCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVLLDADSHASIYDACSLGGAEIIRFRHNDAKDLERRMVRLGERAKEAIIIVEGIYSMLG
CEEECCCCCCHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHH
DVAPLAEIVDIKRRLGGYLIVDEAHSFGVLGATGRGLAEAVGVEDDVDIIVGTFSKSLAS
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCCHHEEHHHHHHHHH
IGGFAVVSEAMEVLRYGSRPYIFTASPSPSCIATVRSSLRTIATQPELRQKLMDNANHLY
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHH
DGLQKLGYELSSHISPVVPVIIGSKEEGLRIWRELISPGVYVNLILPPAAPAGITLLRCS
HHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEC
VNAAHSREQIDAIIQAFATLKQ
CCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2110099 [H]