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The map label for this gene is cysN

Identifier: 30064108

GI number: 30064108

Start: 2840833

End: 2842260

Strand: Reverse

Name: cysN

Synonym: S2967

Alternate gene names: 30064108

Gene position: 2842260-2840833 (Counterclockwise)

Preceding gene: 30064109

Following gene: 30064107

Centisome position: 61.8

GC content: 54.06

Gene sequence:

>1428_bases
ATGAACACCGTACTTGCACAACAAATCGCCAATGAAGGCGGCGTCGAAGCCTGGATGATTGCGCAACAACATAAAAGCCT
GCTGCGTTTTCTGACCTGTGGTAGCGTCGATGACGGCAAAAGTACCCTGATTGGTCGTCTGCTGCACGATACCCGCCAAA
TCTACGAAGATCAGCTCTCATCGCTGCATAACGACAGTAAGCGTCACGGCACCCAGGGCGAAAAGCTGGATCTGGCTCTG
CTGGTGGACGGCCTGCAAGCTGAGCGCGAACAGGGCATCACTATTGATGTGGCCTACCGCTATTTCTCTACCGAGAAGCG
TAAATTTATTATCGCCGACACCCCAGGGCACGAGCAGTACACTCGCAATATGGCGACTGGCGCATCGACATGTGAACTGG
CGATCTTACTGATTGATGCCCGTAAAGGCGTGCTCGATCAAACCCGTCGTCACAGTTTTATCTCCACACTGTTGGGGATC
AAACATCTGGTCGTGGCGATCAACAAAATGGATCTGGTGGATTACAGTGAAGAGACGTTCACCCGTATTCGTGAAGATTA
TCTGACCTTTGCCGGGCAGCTGCCGGGTAATCTGGATATCCGCTTTGTGCCGCTCTCCGCACTGGAAGGTGACAACGTGG
CTTCGCAAAGTGAAAGTATGCCGTGGTACAGCGGTCTGACACTGCTCGAAGTGCTGGAAACCGTGGAGATCCAGCGAGTG
GTGGATGCCCAGCCAATGCGCTTCCCGGTGCAGTACGTTAACCGCCCAAATCTCGATTTTCGTGGCTACGCCGGAATGCT
GGCATCCGGTCGCGTGGAAGTCGGGCAACGTGTAAAAGTGCTGCCCTCTGGTGTGGAATCAAACGTCGCGCGGATCGTGA
CTTTTGATGGTGATCGCGAAGAAGTCTTTGCCGGAGAAGCGATCACCCTGGTGCTGACGGATGAGATCGATATCAGCCGT
GGTGATCTGCTGCTGGCGGCAGACGAAGCGTTACCGGCGGTGCAGAGTGCGTCGGTGGATGTGGTATGGATGGCGGAACA
GCCGCTTTCCCCGGGCCAGAGTTATGACATCAAAATTGCCGGTAAGAAGACGCGTGCTCGTGTTGATGGCATTCGTTATC
AGGTTGATATTAATAACCTTACCCAACGCGAAGTTGAAAACCTGCCGCTGAACGGCATCGGCCTGGTGGATCTCACTTTT
GACGAGCCACTGGTGTTAGATCGCTATCAGCAAAATCCGGTTACCGGTGGGCTGATTTTTATCGATCGCCTGAGCAATGT
GACCGTGGGTGCCGGTATGGTGCACGAGCCAGTTAGCCAGGCAACAGCTGCGTCATCTGAATTCAGTGCATTCGAACTGG
AATTGAATGCCCTGGTTCGTCGCCACTTTCCGCACTGGGGCGCGCGCGATTTGCTGGGGGATAAATAA

Upstream 100 bases:

>100_bases
GAGATGCTGGTTTCCACCACCAGTGAACGTCAGGGTCGCGTGATTGACCGCGACCAGGCGGGATCCATGGAGCTGAAAAA
ACGTCAGGGGTATTTTTAAG

Downstream 100 bases:

>100_bases
TGGCGCTGCATGACGAAAACGTCGTCTGGCATAGTCATCCGGTCACTGTGCAACAACGCGAACTACACCACGGTCATCGT
GGTGTAGTGCTGTGGTTTAC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGLTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSAFELELNALVRRHFPHWGARDLLGDK

Sequences:

>Translated_475_residues
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGLTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSAFELELNALVRRHFPHWGARDLLGDK
>Mature_475_residues
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGLTLLEVLETVEIQRV
VDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQRVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISR
GDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTF
DEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSAFELELNALVRRHFPHWGARDLLGDK

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI194097354, Length=293, Percent_Identity=34.8122866894198, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI194018520, Length=293, Percent_Identity=34.8122866894198, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI194018522, Length=293, Percent_Identity=34.8122866894198, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=28.735632183908, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI46094014, Length=293, Percent_Identity=33.7883959044369, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=28.735632183908, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=27.313769751693, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI4503471, Length=441, Percent_Identity=26.3038548752834, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI34147630, Length=289, Percent_Identity=29.0657439446367, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157426893, Length=351, Percent_Identity=24.7863247863248, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789108, Length=475, Percent_Identity=98.9473684210526, Blast_Score=960, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=321, Percent_Identity=26.791277258567, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789737, Length=321, Percent_Identity=26.791277258567, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI2367247, Length=94, Percent_Identity=38.2978723404255, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115532067, Length=342, Percent_Identity=29.2397660818713, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI115532065, Length=342, Percent_Identity=29.2397660818713, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32566629, Length=426, Percent_Identity=28.4037558685446, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=28.7640449438202, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=28.7640449438202, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=27.3972602739726, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=26.9709543568465, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.231884057971, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=348, Percent_Identity=28.448275862069, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=393, Percent_Identity=29.5165394402036, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=27.6643990929705, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=27.6643990929705, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=437, Percent_Identity=27.6887871853547, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6324761, Length=146, Percent_Identity=34.2465753424658, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=149, Percent_Identity=34.2281879194631, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=28.7671232876712, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553807, Length=275, Percent_Identity=32.3636363636364, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI45553816, Length=275, Percent_Identity=32.3636363636364, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24651721, Length=275, Percent_Identity=32.3636363636364, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17864154, Length=275, Percent_Identity=32.3636363636364, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24652838, Length=441, Percent_Identity=25.8503401360544, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137572, Length=441, Percent_Identity=25.8503401360544, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI17137380, Length=312, Percent_Identity=30.4487179487179, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281363316, Length=143, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17864358, Length=143, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19921738, Length=93, Percent_Identity=40.8602150537634, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52651; Mature: 52651

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGLTLLEVLETVEIQRV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCHH
EVFAGEAITLVLTDEIDISRGDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIA
HEECCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHEECCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPVSQATAASSEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAINKMDLVDYSEETF
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHH
TRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSESMPWYSGLTLLEVLETVEIQRV
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHH
VDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQRVKVLPSGVESNVARIVTFDGDRE
HCCCCCCCCHHHCCCCCCCCCCCCCHHCCCCCCCCCEEEECCCCCCCCEEEEEEECCCHH
EVFAGEAITLVLTDEIDISRGDLLLAADEALPAVQSASVDVVWMAEQPLSPGQSYDIKIA
HEECCCEEEEEEECCCCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDGIRYQVDINNLTQREVENLPLNGIGLVDLTFDEPLVLDRYQQNPVTGGLIF
CCHHHHEECCEEEEEECCCCCHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCEEE
IDRLSNVTVGAGMVHEPVSQATAASSEFSAFELELNALVRRHFPHWGARDLLGDK
EEECCCCEECCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA