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The map label for this gene is hycB

Identifier: 30064078

GI number: 30064078

Start: 2812198

End: 2812809

Strand: Reverse

Name: hycB

Synonym: S2933

Alternate gene names: 30064078

Gene position: 2812809-2812198 (Counterclockwise)

Preceding gene: 30064079

Following gene: 30064077

Centisome position: 61.16

GC content: 57.03

Gene sequence:

>612_bases
GTGAATCGTTTTGTAATTGCCGACTCCACGCTCTGTATCGGCTGCCACACCTGTGAGGCCGCCTGTTCAGAGACGCATCG
CCAGCACGGCCTGCAATCAATGCCGCGCCTGAGAGTGATGCTCAATGAAAAAGAATCTGCGCCGCAGCTCTGTCACCACT
GTGAAGATGCACCCTGCGCGGTGGTCTGCCCGGTTAACGCCATCACCCGCGTTGATGGGGCCGTGCAGTTGAATGAAAGT
CTGTGCGTAAGCTGCAAACTGTGCGGCATCGCCTGCCCGTTTGGCGCAATTGAATTTTCCGGCAGCCGTCCGCTGGATAT
TCCGGCAAACGCCAATACCCCGAAAGCGCCACCGGCACCGCCTGCTCCGGCGCGTGTCAGCACATTGCTTGACTGGGTGC
CAGGTATTCGCGCGATCGCCGTCAAATGTGACCTTTGTAGCTTTGATGAACAAGGTCCGGCCTGTGTGCGGATGTGCCCG
ACTAAAGCCCTGCATCTGGTGGATAACACCGATATCGCCCGCGTCAGCAAACGTAAGCGTGAGCTGACCTTTAACACGGA
CTTTGGCGATCTCACCTTGTTTCAGCAGGCTCAAAGTGGAGAGGCTAAATGA

Upstream 100 bases:

>100_bases
GCGTTACGCCTGTTTGGGGATGGGCGTGTCGATGAGTGTCGAAAATGACATTTCATCGGCATGTTTTCGTCAAAAATGAC
AATCACCTGAGGAATGCCTG

Downstream 100 bases:

>100_bases
GCGCAATTTCCCTGATCAATAGCGGCGTGGCATGGTTTGTCGCCGCCGCTGTTCTGGCATTTCTCTTTTCAAAAAGCGCT
AAGTGGCTGGATAGCCGGAA

Product: small subunit of hydrogenase-3, iron-sulfur protein (part of formate hydrogenlyase (FHL) complex)

Products: Oxidized ferredoxin; CO2; H2 [C]

Alternate protein names: FHL subunit 2; Hydrogenase-3 component B

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEKESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK

Sequences:

>Translated_203_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEKESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK
>Mature_203_residues
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEKESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNES
LCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK

Specific function: Probable electron transfer protein for hydrogenase 3

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1789079, Length=203, Percent_Identity=100, Blast_Score=416, Evalue=1e-118,
Organism=Escherichia coli, GI87082114, Length=186, Percent_Identity=51.0752688172043, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1788811, Length=185, Percent_Identity=38.9189189189189, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI2367245, Length=187, Percent_Identity=39.0374331550802, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1789067, Length=190, Percent_Identity=39.4736842105263, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI87082180, Length=189, Percent_Identity=34.3915343915344, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI87082179, Length=184, Percent_Identity=36.4130434782609, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI2367345, Length=204, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI226510944, Length=98, Percent_Identity=37.7551020408163, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYCB_ECOL6 (P0AAK2)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755163.1
- ProteinModelPortal:   P0AAK2
- SMR:   P0AAK2
- EnsemblBacteria:   EBESCT00000043737
- GeneID:   1039088
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3284
- GeneTree:   EBGT00050000009106
- HOGENOM:   HBG726839
- OMA:   RNEKESA
- ProtClustDB:   CLSK880466
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 21873; Mature: 21873

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
9.9 %Cys+Met (Translated Protein)
7.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
9.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEKESAPQLCHHCEDAPCA
CCEEEEECCEEEEECCHHHHHHHHHHHHHCHHHCCCEEEEECCCCCHHHHHHHCCCCCEE
VVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAP
EEECCHHHHHCCCCEEECCCCEEEEEECEEECCCCCEEECCCCCEECCCCCCCCCCCCCC
PAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCPTKALHLVDNTDIARVSKRKR
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHH
ELTFNTDFGDLTLFQQAQSGEAK
EEEEECCCCCCCEEHHCCCCCCC
>Mature Secondary Structure
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEKESAPQLCHHCEDAPCA
CCEEEEECCEEEEECCHHHHHHHHHHHHHCHHHCCCEEEEECCCCCHHHHHHHCCCCCEE
VVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAP
EEECCHHHHHCCCCEEECCCCEEEEEECEEECCCCCEEECCCCCEECCCCCCCCCCCCCC
PAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCPTKALHLVDNTDIARVSKRKR
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHH
ELTFNTDFGDLTLFQQAQSGEAK
EEEEECCCCCCCEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; H+; formate [C]

Specific reaction: 2 reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 formate = CO2 + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157