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The map label for this gene is hycD

Identifier: 30064076

GI number: 30064076

Start: 2809442

End: 2810365

Strand: Reverse

Name: hycD

Synonym: S2931

Alternate gene names: 30064076

Gene position: 2810365-2809442 (Counterclockwise)

Preceding gene: 30064077

Following gene: 30064075

Centisome position: 61.1

GC content: 58.12

Gene sequence:

>924_bases
ATGAGTGTTTTATATCCGTTAATTCAGGCGCTGGTGTTATTTGCCGTTGCGCCGCTGCTCTCCGGTATTACCCGCGTGGC
GCGCGCCCGTCTGCATAACCGTCGCGGGCCGGGCGTGTTGCAGGAGTATCGCGACATTATCAAACTGCTGGGCCGCCAGA
GCGTTGGCCCGGATGCCTCTGGCTGGATGTTCCGCCTGACGCCGTATGTGATGGTGGGCGTCATGCTGACTATCGCTACT
GCGCTGCCGGTGGTGACCGTCGGTTCTCCGCTGCCGCAACTGGGCGATTTGATCACCTTACTGTATCTCTTCGCCATCGC
GCGTTTCTTCTTTGCCATTTCCGGTCTGGATACCGGTAGCCCGTTTACCGCTATCGGTGCGAGCCGTGAAGCGATGCTTG
GCGTGCTGGTCGAACCGATGTTGCTGCTTGGTCTGTGGGTTGCCGCACAGGTTGCCGGTTCCACCAACATCAGCAACATT
ACCGACACCGTTTATCACTGGCCGCTGAGCCAAAGCATCCCGCTGGTGCTGGCGCTCTGCGCCTGTGCGTTCGCCACTTT
TATCGAAATGGGCAAACTGCCGTTCGACCTGGCGGAAGCCGAGCAGGAGTTGCAGGAAGGTCCGCTCTCCGAATACAGCG
GTAGCGGCTTTGGCGTCATGAAATGGGGTATCAGCCTGAAACAGCTGGTGGTGTTGCAGATGTTCGTCGGGGTGTTTATT
CCGTGGGGACAAATGGAAACCTTCACCGTCGGTGGACTGCTGCTGGCGCTGGTGATTGCCATCGTCAAACTGGTGGTCGG
CGTCCTGGTTATCGCGCTGTTCGAAAACAGCATGGCCCGTCTGCGTCTTGATATTACTCCGCGCATTACCTGGGCTGGGT
TTGGCTTTGCATTTTTAGCGTTCGTCTCCTTGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGGCTGGCAGTGCGAAGGTAGTGCGTTGCTGTTCCGCCGGATGGCGCTGGTTGAACTGGCGGTACTGGTGGTGATTATT
GTTTCACGAGGAGCCTGAGA

Downstream 100 bases:

>100_bases
TTAAAGAGAGTTTGAGCATGTCTGAAGAAAAATTAGGTCAACATTATCTCGCCGCGCTGAATGAGGCATTTCTAGGCGTC
GTGCTGGACCACGCCTGGCA

Product: membrane-spanning protein of hydrogenase 3 (part of FHL complex)

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA

Sequences:

>Translated_307_residues
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA
>Mature_306_residues
SVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATA
LPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNIT
DTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFIP
WGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=307, Percent_Identity=99.3485342019544, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI87082115, Length=303, Percent_Identity=52.4752475247525, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1788618, Length=306, Percent_Identity=25.4901960784314, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 33090; Mature: 32959

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00667 COMPLEX1_ND1_1 ; PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLVLALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCEEHHHHHHHHHH
FVSLLAA
HHHHHCC
>Mature Secondary Structure 
SVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLVLALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCEEHHHHHHHHHH
FVSLLAA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]