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The map label for this gene is napC

Identifier: 30063642

GI number: 30063642

Start: 2299115

End: 2299717

Strand: Reverse

Name: napC

Synonym: S2416

Alternate gene names: 30063642

Gene position: 2299717-2299115 (Counterclockwise)

Preceding gene: 30063643

Following gene: 30063641

Centisome position: 50.0

GC content: 51.74

Gene sequence:

>603_bases
ATGGGAAATTCTGACCGTAAGCCTGGTCTGATTAAGCGCCTGTGGAAATGGTGGCGTACCCCCAGCCATCTGGCGCTGGG
GACGCTGCTGTTGATCGGTTTTGTTGGCGGCATCGTCTTCTGGGGGGGCTTTAACACCGGGATGGAAAAAGCCAATACCG
AAGAGTTCTGCATTAGCTGCCACGAAATGCGCAACACGGTGTATCAGGAATACATGGATTCCGTGCACTACAACAACCGT
AGCGGCGTCCGTGCGACCTGTCCGGATTGTCACGTTCCGCACGAGTTTGTGCCGAAGATGATACGCAAGCTCAAAGCAAG
TAAAGAGCTGTATGGTAAAATTTTTGGCGTTATTGACACGCCGCAGAAATTTGAAGCTCATCGTCTGACGATGGCACAGA
ATGAGTGGCGGCGCATGAAGGACAATAACTCGCAGGAGTGCCGTAACTGTCACAACTTCGAGTATATGGATACAACCGCC
CAGAAATCGGTTGCCGCGAAGATGCATGACCAGGCGGTGAAAGATGGGCAAACCTGTATTGATTGCCATAAAGGGATCGC
GCACAAGCTGCCCGATATGCGTGAAGTCGAGCCAGGTTTTTAA

Upstream 100 bases:

>100_bases
TTATTTCTGTCTGCAATGTCACGTACCGCAGGCCGATACCGCGCCAATCGTGGGGAATACCTTTACCCCATCAAAAGGTT
ACGGGAAATAAGAGGTTATT

Downstream 100 bases:

>100_bases
CAGGGTTATTGCGTGGGTATGCTTGAAGCCAGAGAGTTACTTTGTGAGCGGGATGAACGAACCTTATTTAGTGGCTTGTC
ATTTACGCTGAACGCAGGAG

Product: cytochrome c-type protein NapC

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNR
SGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF

Sequences:

>Translated_200_residues
MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNR
SGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF
>Mature_199_residues
GNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRS
GVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTAQ
KSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF

Specific function: Mediates electron flow from quinones to the NapAB complex [H]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788530, Length=200, Percent_Identity=99.5, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1787230, Length=186, Percent_Identity=49.4623655913978, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788181, Length=148, Percent_Identity=45.945945945946, Blast_Score=152, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005126
- InterPro:   IPR011885 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 23082; Mature: 22951

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
9.0 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISC
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHH
HEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDT
HHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTAQKSVAAKMHDQAVKDGQTCI
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
DCHKGIAHKLPDMREVEPGF
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISC
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHH
HEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDT
HHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTAQKSVAAKMHDQAVKDGQTCI
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
DCHKGIAHKLPDMREVEPGF
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]