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The map label for this gene is yohI

Identifier: 30063584

GI number: 30063584

Start: 2231683

End: 2232630

Strand: Reverse

Name: yohI

Synonym: S2354

Alternate gene names: 30063584

Gene position: 2232630-2231683 (Counterclockwise)

Preceding gene: 30063591

Following gene: 30063582

Centisome position: 48.54

GC content: 54.32

Gene sequence:

>948_bases
ATGCGTGTGTTACTGGCACCGATGGAGGGAGTGCTTGACTCTCTGGTGCGTGAATTGCTGACCGAAGTTAACGACTACGA
TCTGTGCATCACCGAGTTTGTCCGCGTGGTGGATCAACTGCTGCCGGTAAAAGTCTTTCATCGCATTTGCCCTGAGCTAC
AAAACGCCAGCCGGACACCATCTGGTACGCTGGTGCGCGTGCAGTTGTTAGGTCAGTTCCCACAATGGCTGGCAGAGAAC
GCCGCCCGTGCGGTGGAGTTAGGTTTCTGGGGCGTGGATCTCAATTGCGGCTGCCCGTCGAAAACGGTTAACGGTAGCGG
CGGCGGGGCGACGTTACTCAAAGATCCTGAACTCATCTACCAGGGGGCAAAAGCGATGCGTGAAGCTGTACCGGCGCATT
TGCCTGTCAGCGTGAAAGTGCGTCTGGGCTGGGACAGCGGTGAGAAGAAATTTGAAATCGCCGATGCGGTTCAACAGGCT
GGCGCTACGGAGCTGGTGGTGCATGGGCGGACGAAAGAGCAGGGTTACCGCGCGGAGCATATTGACTGGCAGGCGATTGG
CGAGATTCGCCAGCGGCTGAATATTCCGGTGATTGCCAACGGTGAAATCTGGGACTGGCAGAGCGCGCAACAATGCATGG
CGATCAGCGGCTGCGACGCAGTGATGATTGGTCGCGGGGCGCTCAATATTCCCAACCTGAGCCGGGTGGTAAAATATAAC
GAACCGCGAATGCCGTGGCCGGAGGTGGTTGCTTTGCTGCAAAAATATACCCGTCTGGAAAAGCAGGGCGATACCGGGTT
ATATCACGTTGCGCGGATTAAACAGTGGTTGAGTTATTTGCGTAAAGAATACGATGAAGCAACGGAATTATTTCAGCATG
TTCGGGTGTTGAATAACTCCCCTGATATTGCAAGGGCTATTCAGGCAATTGATATCGAGAAACTCTAA

Upstream 100 bases:

>100_bases
ATAGAGAGGGATTGCAGTTTGCAGCAATCCCTCTTTGCAGACCTTTAATCCGCTGACCATAGGCGCTAACATAGCGCCTC
ATTTTTTTGCGGGTGATGAT

Downstream 100 bases:

>100_bases
AAACCATAGTGATGTTTTTGACAATGTGTTAGCTCTTTATCAATAGGCCGCACGGTTGTTTGGTGGCGTCAAAATCGCCG
AGGCGTTCCCTGAAGGCCGG

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGFWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL

Sequences:

>Translated_315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGFWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL
>Mature_315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGFWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=31.1688311688312, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI239788483, Length=225, Percent_Identity=32.4444444444444, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI8923374, Length=269, Percent_Identity=28.6245353159851, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI239788462, Length=225, Percent_Identity=32.4444444444444, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=28.8702928870293, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788462, Length=315, Percent_Identity=99.3650793650794, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=28.7037037037037, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI145693211, Length=251, Percent_Identity=25.8964143426295, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=168, Percent_Identity=38.6904761904762, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=35.0649350649351, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=271, Percent_Identity=25.4612546125461, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=201, Percent_Identity=31.3432835820896, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=146, Percent_Identity=27.3972602739726, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585320, Length=219, Percent_Identity=30.5936073059361, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24580595, Length=148, Percent_Identity=31.7567567567568, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920448, Length=148, Percent_Identity=31.7567567567568, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35274; Mature: 35274

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTP
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKTVNGSGGGATLLKDPELIY
CCCEEEHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCEEECCHHHHH
QGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
IDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIARAIQAIDIEKL
CHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTP
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKTVNGSGGGATLLKDPELIY
CCCEEEHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCEEECCHHHHH
QGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
IDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRVLNNS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIARAIQAIDIEKL
CHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]