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The map label for this gene is wza

Identifier: 30063511

GI number: 30063511

Start: 2124329

End: 2125468

Strand: Reverse

Name: wza

Synonym: S2250

Alternate gene names: 30063511

Gene position: 2125468-2124329 (Counterclockwise)

Preceding gene: 30063513

Following gene: 30063510

Centisome position: 46.21

GC content: 53.25

Gene sequence:

>1140_bases
ATGATGAAATCCAAAATGAAATTGATGCCATTATTGGTGTCAGTAACCTTGATAAGCGGTTGCACAGTACTTCCGGGCAG
CAATATGTCGACGATGGGCAAAGACGTCATCAAACAGCAGGACGCTGATTTCGATCTCGACAAAATGGTGAATGTTTATC
CGCTGACCCCGCGCCTGATTGACCAATTACGCCCACGCCCGAATGTAGCGCGCCCCAATATGACGCTGGAAAGCGAGATC
GCGAATTACCAGTATCGCGTCGGGCCGGGGGACGTTCTTAATGTCACCGTCTGGGATCACCCGGAACTCACCACGCCAGC
TGGTCAGTACCGCAGCTCCAGCGACACCGGCAACTGGGTACAGCCTGACGGCACTATGTTTTACCCGTATATCGGCAAGG
TTCACGTAGTCGGGAAAACGCTCGCTGAAATCCGCAGTGATATTACCGGGCGCTTAGCGACGTACATCGCTGACCCGCAG
GTGGACGTTAATATCGCCGCCTTCCGCTCACAAAAGGCCTATATCTCAGGTCAGGTGAATAAATCCGGTCAACAGGCGAT
CACCAACGTACCACTGACCATTCTCGACGCCATCAACGCCGCAGGTGGCCTGACCGACACCGCTGACTGGCGCAACGTGG
TGCTAACACACAATGGTCGTGAAGAACGCATTTCTTTACAGGCGCTGATGCAAAACGGCGACCTCAATCAGAACCGACTG
CTTTATCCCGGCGATATTCTCTACGTGCCGCGTAATGATGATCTGAAAGTATTTGTGATGGGTGAAGTGAAGAAACAGAG
CACCCTGAAAATGGACTTTAGCGGCATGACCCTGACTGAAGCTCTGGCCAATGCCGAAGGTATCGACATGACCACCTCCA
ACGCCAGCGGCATCTTTGTCATTCGTCCGCTGAAAGGCGAGGGCGGGCGTAACGGCAAGATTGCCAATATCTACCAGCTG
GATATGTCCGATGCGACGTCGCTGGTGATGGCGACAGAATTCCGCCTGCAACCTTATGACGTGGTGTATGTCACCACCGC
CCCGGTTTCCCGCTGGAACCGTCTGATCAATCAGTTGCTGCCAACTATTAGCGGTGTCCGTTACATGACGGATACAGCCA
GCGACATTCATAACTGGTAA

Upstream 100 bases:

>100_bases
AGTTAAATTTTTATTCAATTAACTGCAAGGTAAATGTGCATGGCGACATTGCTATTAATAGTGCACAGGATAATTACTCT
GCCAAAGTGATAAATAAACA

Downstream 100 bases:

>100_bases
TCGTCATGTTTAACAACATCTTAGTTGTCTGTGTCGGCAATATTTGCCGTTCCCCGATGGCGGAACGCTTACTGCAACGT
TATCACCCGGAACTGAAAGT

Product: putative polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEI
ANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQL
DMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW

Sequences:

>Translated_379_residues
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEI
ANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQL
DMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW
>Mature_379_residues
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEI
ANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQL
DMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW

Specific function: Probably involved in the export of the extracellular polysaccharide colanic acid from the cell to medium [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=379, Percent_Identity=99.7361477572559, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1787218, Length=379, Percent_Identity=63.8522427440633, Blast_Score=515, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 41925; Mature: 41925

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLI
CCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCCHHHCCEEEECCHHHH
DQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWV
HHCCCCCCCCCCCCEEHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEE
QPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQVDVNIAAFRSQKAYISGQVN
CCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCEEEEECCC
KSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
CCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEE
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALANAEGIDMTTSNASGIFV
ECCCCEEEEECCCCEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCCEEECCCCCCEEE
IRPLKGEGGRNGKIANIYQLDMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLL
EEECCCCCCCCCCEEEEEEECCCCCCEEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHH
PTISGVRYMTDTASDIHNW
HHHCCCEEEECCHHHHCCC
>Mature Secondary Structure
MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADFDLDKMVNVYPLTPRLI
CCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCCHHHCCEEEECCHHHH
DQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPELTTPAGQYRSSSDTGNWV
HHCCCCCCCCCCCCEEHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEE
QPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQVDVNIAAFRSQKAYISGQVN
CCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCEEEEECCC
KSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHNGREERISLQALMQNGDLNQNRL
CCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEE
LYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGMTLTEALANAEGIDMTTSNASGIFV
ECCCCEEEEECCCCEEEEEEECCCCCCEEEEECCCCHHHHHHCCCCCCEEECCCCCCEEE
IRPLKGEGGRNGKIANIYQLDMSDATSLVMATEFRLQPYDVVYVTTAPVSRWNRLINQLL
EEECCCCCCCCCCEEEEEEECCCCCCEEEEEEEEECCCEEEEEEEECCHHHHHHHHHHHH
PTISGVRYMTDTASDIHNW
HHHCCCEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]