Click here to switch to the map view.

The map label for this gene is wcaM

Identifier: 30063494

GI number: 30063494

Start: 2102996

End: 2104390

Strand: Reverse

Name: wcaM

Synonym: S2229

Alternate gene names: 30063494

Gene position: 2104390-2102996 (Counterclockwise)

Preceding gene: 30063495

Following gene: 30063493

Centisome position: 45.75

GC content: 47.96

Gene sequence:

>1395_bases
ATGCCATTTAAAACACTCTCCCGACGCACCTTCCTGACGGCAAGCTCGGCGCTTGCCTTCCTCCATACCCCTTTCGCTCG
CGCACTTCCCGCCCGACAAAGCGTTAACATTAACGACTACAACCCACATGACTGGATCGCCTCATTTAAACAAGCCTTCA
GCGAAGGGCAAACGGTCGTCGTGCCTGCCGGATTCGTTTGTGACAATATCAACACCGGCATCTTCATCCCTCCCGGCAAA
ACGTTACACATCCTTGGAAGCCTGCGCGGCAACGGCAGAGGGCGATTTGTCTTACAGGACGGCAGCCAGGTGACAGGGGG
AGAGGGCGGCGGTATGCATAACATTACCCTGGATGTGCGTGGTTCTGACTGCACCATCAAAGGGCTGGCGATGAGCGGCT
TTGGCCCGGTAATGCAGATTTATATTGGCGGCAAAAACAAACGGGTCATGCGCAACCTGACCATCGATAACCTCACTGTC
AGCCACGCTAATTACGCCATCTTACGCCAGGGATTTCATAACCAGATTATCGGTGCCAACATTACCAACTGTAAGTTCAG
CGACTTACAGGGCGACGCCATTGAATGGAACGTGGCAATTAACGACAGTGATATTTTGATATCTGACCATGTCATCGAGC
GTATCAACTGTACCAACGGCAAAATCAACTGGGGCATCGGCATAGGCCTTGCGGGAAGCACTTATGATAACAACTACCCG
GAAGACCAGGCAGTGAAAAACTTTGTCGTGGCGAATATCACGGGATCGGATTGTCGGCAGTTGATCCATGTTGAAAATGG
TAAACATTTTGTTATTCGTAATATCAATGCCCGCAATATCACGCCGGATTTCAGTAAGAAAGCGGGCATTGATAACGCGA
CAGTCGCTATTTACGGTTGTGACAATTTCGTGATTGATAATATTGAAATGATTAATAGCGCCGGGATGTTAATCGGCTAT
GGGGTAATTAAAGGCAAATATCTCTCGATACCGCAAAATTTCCGAGTGAATAATATTCAACTGGATAATACCCATCTTGC
TTATAAATTGCGCGGCATCCAAATCTCCGCCGGGAATGCCGTCTCCTTTGTGTCGCTAACTAACATTGAGATGAAGCGTG
CCTCGCTGGAGTTACACAACAAGCCGCAACATCTTTTTATGCGAAATATCAATGTGATGCAGGAATCCTCAGTTGGACCA
GCATTGAGCATGAACTTCGACATGCGCAAAGACGTTCGCGGCGTCTTTATGGCGAAAAAAGAAACACTGCTGTCTCTTGC
AAATGTTCATGCGGTGAATGAAAGAGGGCAAAGCTCCGTCGATATCGACAGGATTAATCACCATATTGTTAATGTGGAAA
AGATTAACTTTAGATTGCCGGAACGGAGGGAGTAG

Upstream 100 bases:

>100_bases
GTCGTCAAACGTGCGTGCGAAAAAGTCGAACACGATTTTAACCAGCAGGTGATTAATCGAGAACTCGCCAGCTTGCTGCA
GGCTTTATAGAGGAGAACGT

Downstream 100 bases:

>100_bases
ATTTGCGACCATTCCTGGAAAAATGGAGCCATACTTAGGAACAATGCTACTGCAATCCACAACGAAGCGGCGTAACATCA
CAAGTAATTCAGTAATCAAT

Product: putative colanic acid biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNINARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE

Sequences:

>Translated_464_residues
MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGK
TLHILGSLRGNGRGRFVLQDGSQVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNINARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGY
GVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINVMQESSVGP
ALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE
>Mature_463_residues
PFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKT
LHILGSLRGNGRGRFVLQDGSQVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTVS
HANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYPE
DQAVKNFVVANITGSDCRQLIHVENGKHFVIRNINARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYG
VIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINVMQESSVGPA
LSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788356, Length=464, Percent_Identity=96.7672413793103, Blast_Score=924, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011050 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51192; Mature: 51061

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVV
CCCCCCCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGGEGGGMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCCEEEECHHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNINARNITPDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNA
CCEEECCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVSLTNIEMKRASLELHNKPQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECEEEEEEEEEECCCCCEEHHHCCCEEECCCCCCEEEECCCHHHCCCEEEEEHH
ETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE
HHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
PFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHDWIASFKQAFSEGQTVV
CCCCCCCEEEEEHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
VPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGSQVTGGEGGGMHNITLDVR
EECCEEECCCCCCEEECCCCEEEEEEECCCCCCEEEEEECCCEECCCCCCCEEEEEEEEE
GSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTVSHANYAILRQGFHNQIIGAN
CCCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECEEEECCCCHHHHHCCCCCCCCCCC
ITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCTNGKINWGIGIGLAGSTYDNNYP
CCCCEECCCCCCEEEEEEEECCCCEEEECHHHHEEECCCCEEEEEEEEEECCCCCCCCCC
EDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNINARNITPDFSKKAGIDNATVAIYGC
CHHHHCEEEEEECCCHHHHEEEEECCCCEEEEEECCCCCCCCCCHHHCCCCCCEEEEEEC
DNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNA
CCEEECCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEECCCEEEEEEEEEEEECCCE
VSFVSLTNIEMKRASLELHNKPQHLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKK
EEEEEEECEEEEEEEEEECCCCCEEHHHCCCEEECCCCCCEEEECCCHHHCCCEEEEEHH
ETLLSLANVHAVNERGQSSVDIDRINHHIVNVEKINFRLPERRE
HHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]