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The map label for this gene is rfbC

Identifier: 30063489

GI number: 30063489

Start: 2098085

End: 2098630

Strand: Reverse

Name: rfbC

Synonym: S2223

Alternate gene names: 30063489

Gene position: 2098630-2098085 (Counterclockwise)

Preceding gene: 30063490

Following gene: 30063488

Centisome position: 45.63

GC content: 37.73

Gene sequence:

>546_bases
ATGAACGTAATTAAAACTGAAATTCCTGATGTGCTAATTTTTGAACCAAAAGTATTTGGTGATGAGCGGGGCTTTTTTAT
GGAAAGCTTCAATCAGAAAGTTTTCGAAGAGGCTGTAGGACGAAAGGTTGAGTTTGTTCAGGATAACCATTCTAAATCAA
CTAAGGGTGTGTTACGCGGACTGCACTATCAGCTGGAACCTTATGCTCAAGGAAAACTGGTACGCTGTGTTGTCGGTGAA
GTTTTTGACGTAGCTGTTGATATTCGGAAATCATCACCAACATTTGGCAAGTGGGTTGGTGTAAATTTGTCGGCGGAGAA
TAAGCGTCAATTATGGATACCGGAAGGATTCGCTCATGGGTTTTGCGTTCTTAGTGATGAAGCAGAATTTGTCTACAAAA
CAAATAATTTCTATTCAAAAATGCAAGAGCGTGGGATTTTATGGAGCGACAAAAGTATAAATATAGAATGGCCGGTTCAA
AATCCATTGCTTTCTGATAAAGATATTAATGGTCAAAAATTTGTAGATGCTGATTATTTTATATGA

Upstream 100 bases:

>100_bases
AGGCTTTATTGATGCTGAGCAAGTGAAGGTATTAGCCGAGCCGCTGAAGAAAAATGCTTATGGTCAGTATCTGCTGAAAA
TGATTAAAGGTTATTAATAA

Downstream 100 bases:

>100_bases
TAAAGAAATGTAATAATGAGCATAATAAAAAATAGTGTCTGGAACCTTTTTGGCTATGCAATACCAACTTTAATTGCTAT
CCCCTCGCTAGGATTTCTCG

Product: dTDP-6-deoxy-L-mannose-dehydrogenase

Products: NA

Alternate protein names: Thymidine diphospho-4-keto-rhamnose 3,5-epimerase; dTDP-4-keto-6-deoxyglucose 3,5-epimerase; dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase; dTDP-L-rhamnose synthase

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVEFVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGE
VFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
NPLLSDKDINGQKFVDADYFI

Sequences:

>Translated_181_residues
MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVEFVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGE
VFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
NPLLSDKDINGQKFVDADYFI
>Mature_181_residues
MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVEFVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGE
VFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
NPLLSDKDINGQKFVDADYFI

Specific function: Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4- hexulose

COG id: COG1898

COG function: function code M; dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family

Homologues:

Organism=Escherichia coli, GI1788350, Length=169, Percent_Identity=66.8639053254438, Blast_Score=228, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17550412, Length=171, Percent_Identity=51.4619883040936, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RMLC_SHIFL (P37780)

Other databases:

- EMBL:   X71970
- EMBL:   L14842
- EMBL:   AE005674
- EMBL:   AE014073
- PIR:   E55213
- RefSeq:   NP_707933.1
- RefSeq:   NP_837660.1
- ProteinModelPortal:   P37780
- SMR:   P37780
- EnsemblBacteria:   EBESCT00000086867
- EnsemblBacteria:   EBESCT00000092938
- GeneID:   1026775
- GeneID:   1078525
- GenomeReviews:   AE005674_GR
- GenomeReviews:   AE014073_GR
- KEGG:   sfl:SF2101
- KEGG:   sfx:S2223
- GeneTree:   EBGT00050000012010
- HOGENOM:   HBG730537
- OMA:   RGAIWDV
- ProtClustDB:   CLSK905328
- BioCyc:   SFLE198214:AAN43640.1-MONOMER
- BRENDA:   5.1.3.13
- InterPro:   IPR011051
- InterPro:   IPR000888
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- PANTHER:   PTHR21047
- ProDom:   PD001462
- TIGRFAMs:   TIGR01221

Pfam domain/function: PF00908 dTDP_sugar_isom; SSF51182 RmlC_like_cupin

EC number: =5.1.3.13

Molecular weight: Translated: 20823; Mature: 20823

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 28-28 BINDING 47-47 BINDING 49-49 BINDING 59-59 BINDING 62-62 BINDING 72-72 BINDING 83-83 BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVEFVQDNHSKSTKGVLRG
CCCCCCCCCCEEEECCEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
LHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHG
HCEEECCCCCCCEEEEHHHHHHHHEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCC
FCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQNPLLSDKDINGQKFVDADYF
EEEEECCCEEEEECCHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCCCCCEEECCCCC
I
C
>Mature Secondary Structure
MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVEFVQDNHSKSTKGVLRG
CCCCCCCCCCEEEECCEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
LHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLSAENKRQLWIPEGFAHG
HCEEECCCCCCCEEEEHHHHHHHHEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCC
FCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQNPLLSDKDINGQKFVDADYF
EEEEECCCEEEEECCHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCCCCCEEECCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8170390; 8157605; 12384590; 12704152