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The map label for this gene is rfbE

Identifier: 30063488

GI number: 30063488

Start: 2096813

End: 2098069

Strand: Reverse

Name: rfbE

Synonym: S2222

Alternate gene names: 30063488

Gene position: 2098069-2096813 (Counterclockwise)

Preceding gene: 30063489

Following gene: 30063487

Centisome position: 45.62

GC content: 34.37

Gene sequence:

>1257_bases
ATGAGCATAATAAAAAATAGTGTCTGGAACCTTTTTGGCTATGCAATACCAACTTTAATTGCTATCCCCTCGCTAGGATT
TCTCGCTCGAGGTTTGGGGCCTGAAGGTTTCGGTGTTTATACAATTGCAATTGCACTTGTTGGGTATGCTGGAATTTTTG
ATGTAGGCCTGACTCGCTCTGTTATTAGAGAAATTGCAATTCATCGTGATAATCATCATGAAAGAACCAAGGTAATTTCA
ACAAGTACATCTTTTTTGGTGCTATTTTCATGCTTTGGTGCTTTTTTATTATTGATTTTCTCTGATGGAATTGTTAATTA
TCTAAAAATTTCTGGTGTTGAACATAGTGATATACAACTAGCATTTAAACTGTTGGCTATTTGCATTCCATTATTTATTC
TAAATCAATTATGGTCAGCCATTCTTGAGGGGGATGAAAAATTTGGCATTGTAAATATTCAAAAATCTATATCAAGCTCT
TGCATCGCGGGAATTCCGGCCATATTTGTTTTTTATAGTGCTACATTGTCGGCAGCGGTTGCTGGTTTAATATTTGCAAG
AGTTATTTCGATTTTAGTCTCTGCCTATTATGTCAGGAATGATATTAAAATTTCGGGGGTTCATTTTTGTTATAAAACCT
TTAAACGACTCTTTTTCTTTGGCGGTTGGATGACAGTAAGTAATATCATAAGTCCGGTCATGGTGTATTTTGATCGATTT
ATAGTGTCAAATATTATGGGGGCAGATAAAGTTGCATTTTATTCTGCACCAGCGGAGGTTATTCTAAAATTAGGAATAAT
ACCTGCAGCAATCGGGAGGGCAGTGTTTCCAAGGTTAAGTAACATCAAAGACTTTAAAGAATTTAAACGTAATGTAAATA
AATCATTGCTTTTAATGTTTCTAATCTGTTTGCCGGTGATAATCATAGGCTTGTTATATTCAGGGCTTGTATTGAAAATA
TGGTTTGGTGAGAATTATCAAATTAATTCCTTTAATATATTAAATGTGTTATTGATCGGTTTTTTCTTCAATGCGCTGGC
AATGATACCATTCTCTGCAATCCAGGCATTAGGAAAATCTAAAATTACTGCTTTGATTCATTGTGCTGAATTGGTTCCTT
ATTTAGCCCTTTTGTATTTTATGGTCGAAAAATATGGGTTACTGGGTGCCGCAATATCCTGGAGCATACGTGTAATTTTA
GATGCTCTGTTATTACAATGGCTTTATACTAGAATGTGTTCTGTATATGAAAACTAA

Upstream 100 bases:

>100_bases
TATAGAATGGCCGGTTCAAAATCCATTGCTTTCTGATAAAGATATTAATGGTCAAAAATTTGTAGATGCTGATTATTTTA
TATGATAAAGAAATGTAATA

Downstream 100 bases:

>100_bases
AATTAACCAATTACTGAGTCACTTGGATAATGAATAGTAATATTTACGCTGTCATTGTGACATATAATCCCGAACTTAAA
AATCTGAATGCACTGATCAC

Product: polysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVIS
TSTSFLVLFSCFGAFLLLIFSDGIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLFFFGGWMTVSNIISPVMVYFDRF
IVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKI
WFGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVIL
DALLLQWLYTRMCSVYEN

Sequences:

>Translated_418_residues
MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVIS
TSTSFLVLFSCFGAFLLLIFSDGIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLFFFGGWMTVSNIISPVMVYFDRF
IVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKI
WFGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVIL
DALLLQWLYTRMCSVYEN
>Mature_417_residues
SIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVIST
STSFLVLFSCFGAFLLLIFSDGIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSSC
IAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLFFFGGWMTVSNIISPVMVYFDRFI
VSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIW
FGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILD
ALLLQWLYTRMCSVYEN

Specific function: Could be an O-antigen transporter

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFBE_SHIFL (P37781)

Other databases:

- EMBL:   X71970
- EMBL:   AE005674
- EMBL:   AE014073
- PIR:   JC4069
- RefSeq:   NP_707932.1
- RefSeq:   NP_837659.1
- EnsemblBacteria:   EBESCT00000087757
- EnsemblBacteria:   EBESCT00000091633
- GeneID:   1026640
- GeneID:   1078524
- GenomeReviews:   AE005674_GR
- GenomeReviews:   AE014073_GR
- KEGG:   sfl:SF2100
- KEGG:   sfx:S2222
- GeneTree:   EBGT00050000009555
- HOGENOM:   HBG448770
- OMA:   GHASIKV
- ProtClustDB:   CLSK905327
- BioCyc:   SFLE198214:AAN43639.1-MONOMER
- InterPro:   IPR002797

Pfam domain/function: PF01943 Polysacc_synt

EC number: NA

Molecular weight: Translated: 46399; Mature: 46268

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12224f60)-; HASH(0x12c6461c)-; HASH(0x13442f24)-; HASH(0x135679a0)-; HASH(0x1373e780)-; HASH(0x10a952c8)-; HASH(0x13583a10)-; HASH(0xfa556e0)-; HASH(0x1344c0a0)-; HASH(0x1357c7cc)-; HASH(0x1334742c)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIFDVGLTRS
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSDGIVNYLKISGVEHSDIQL
HHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHH
AFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSSCIAGIPAIFVFYSATLSAAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLFFFGGWMTVSNIISPVMVYFDRF
HHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMF
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LICLPVIIIGLLYSGLVLKIWFGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKS
HHHHHHHHHHHHHHHHEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIFDVGLTRS
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSDGIVNYLKISGVEHSDIQL
HHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHH
AFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSSCIAGIPAIFVFYSATLSAAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLFFFGGWMTVSNIISPVMVYFDRF
HHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLSNIKDFKEFKRNVNKSLLLMF
HHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LICLPVIIIGLLYSGLVLKIWFGENYQINSFNILNVLLIGFFFNALAMIPFSAIQALGKS
HHHHHHHHHHHHHHHHEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7507920; 12384590; 12704152