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The map label for this gene is rfbI

Identifier: 30063484

GI number: 30063484

Start: 2093449

End: 2093823

Strand: Reverse

Name: rfbI

Synonym: S2218

Alternate gene names: 30063484

Gene position: 2093823-2093449 (Counterclockwise)

Preceding gene: 30063485

Following gene: 30063483

Centisome position: 45.52

GC content: 30.13

Gene sequence:

>375_bases
ATGTTAAAAATAGGGAAGTTATTGACCAGTTCTTTTTTTAGTTATTTTTTAATTGGAATTGTCAATACTGCTTTACATTG
GGGGGTATTTTATGCGTGTTATAATAATTTAGCTTTTGGTCAAGGCCGAAGTAACATTGTTGGTTTTATATGTGCAGCAA
CCTTTTCTTTTTTTGCAAATGCAAGATGCTCATTTAAAGTATCTGCAACCAAGGCTAGATATTTTATATTTATATTTTTT
ATGGGGGCGATGAGTTATTTATTCGGCGTTTTATTTGATCTCTTAGCTTTATCTCCAATATTTACATTGTTCACCTTTTC
TCTTTTTAGTTTGGTTTTAGGATATTGCGCATCAAAATATTTCATTTTTCGTTAG

Upstream 100 bases:

>100_bases
ACCCATGGGAATTTTTATTATAGGCGTTGTAGTTTTATGGGTATTTTCTATAGAAAATATCCAAATTAGTAATAACCTCA
CTTCTGGAGCAAAATAAAGG

Downstream 100 bases:

>100_bases
TAAACGATTTGTTAAAAACACAATTACTTGCACATTAGCTAAATAGTTTATATGAGGGGCTTATGTATCACTCTTTTAAT
ATCGCTGTAATTATTCCTTG

Product: glycosyl translocase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRSNIVGFICAATFSFFANARCSFKVSATKARYFIFIFF
MGAMSYLFGVLFDLLALSPIFTLFTFSLFSLVLGYCASKYFIFR

Sequences:

>Translated_124_residues
MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRSNIVGFICAATFSFFANARCSFKVSATKARYFIFIFF
MGAMSYLFGVLFDLLALSPIFTLFTFSLFSLVLGYCASKYFIFR
>Mature_124_residues
MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRSNIVGFICAATFSFFANARCSFKVSATKARYFIFIFF
MGAMSYLFGVLFDLLALSPIFTLFTFSLFSLVLGYCASKYFIFR

Specific function: Involved in O antigen modification. Involved in the translocation of bactoprenol-linked glucose across the cytoplasmic membrane

COG id: COG2246

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gtrA family

Homologues:

Organism=Escherichia coli, GI1788691, Length=113, Percent_Identity=45.1327433628319, Blast_Score=94, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GTRA_SHIFL (P37785)

Other databases:

- EMBL:   X71970
- EMBL:   AE005674
- EMBL:   AE014073
- PIR:   D36966
- RefSeq:   NP_707928.1
- RefSeq:   NP_837655.1
- EnsemblBacteria:   EBESCT00000086422
- EnsemblBacteria:   EBESCT00000091393
- GeneID:   1025291
- GeneID:   1078520
- GenomeReviews:   AE005674_GR
- GenomeReviews:   AE014073_GR
- KEGG:   sfl:SF2096
- KEGG:   sfx:S2218
- GeneTree:   EBGT00050000012127
- HOGENOM:   HBG416262
- ProtClustDB:   CLSK891775
- BioCyc:   SFLE198214:AAN43635.1-MONOMER
- GO:   GO:0006810
- InterPro:   IPR016480
- InterPro:   IPR007267
- PIRSF:   PIRSF006298

Pfam domain/function: PF04138 GtrA

EC number: NA

Molecular weight: Translated: 14033; Mature: 14033

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1345085c)-; HASH(0x12a8d9b0)-; HASH(0x135b6778)-; HASH(0x1380d104)-;

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRSNIVGFICAATFSFFAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
ARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFTLFTFSLFSLVLGYCASKY
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIFR
HCCH
>Mature Secondary Structure
MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRSNIVGFICAATFSFFAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
ARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFTLFTFSLFSLVLGYCASKY
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIFR
HCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7507920; 12384590; 12704152