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The map label for this gene is insB1 [H]

Identifier: 30063466

GI number: 30063466

Start: 2077971

End: 2078264

Strand: Reverse

Name: insB1 [H]

Synonym: S2199

Alternate gene names: 30063466

Gene position: 2078264-2077971 (Counterclockwise)

Preceding gene: 30063467

Following gene: 30063465

Centisome position: 45.19

GC content: 52.04

Gene sequence:

>294_bases
GTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGT
ATGGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGC
GCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGTACGGAAGTCACTGTCGTTCTCAAAATCGGTG
GAGCTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
GATTGTCTGCGCTGAAATGGACGAACATTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATG
ACAGGATACGGAGGACGGTT

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCTATTGAGTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGA
TAAGCGAATTGTTAAAAAGA

Product: IS1 orfB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 97; Mature: 96

Protein sequence:

>97_residues
MAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSV
ELHDKVIGHYLNIKHYQ

Sequences:

>Translated_97_residues
MAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSV
ELHDKVIGHYLNIKHYQ
>Mature_96_residues
AHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVE
LHDKVIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=97, Percent_Identity=91.7525773195876, Blast_Score=186, Evalue=4e-49,
Organism=Escherichia coli, GI1788203, Length=97, Percent_Identity=91.7525773195876, Blast_Score=186, Evalue=4e-49,
Organism=Escherichia coli, GI1786203, Length=97, Percent_Identity=91.7525773195876, Blast_Score=186, Evalue=4e-49,
Organism=Escherichia coli, GI1787223, Length=97, Percent_Identity=90.7216494845361, Blast_Score=184, Evalue=1e-48,
Organism=Escherichia coli, GI1786470, Length=97, Percent_Identity=90.7216494845361, Blast_Score=183, Evalue=2e-48,
Organism=Escherichia coli, GI1786459, Length=97, Percent_Identity=90.7216494845361, Blast_Score=183, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 11509; Mature: 11377

Theoretical pI: Translated: 10.80; Mature: 10.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCC
NLRQHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ
CHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCC
NLRQHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ
CHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]