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The map label for this gene is nac

Identifier: 30063441

GI number: 30063441

Start: 2053693

End: 2054610

Strand: Reverse

Name: nac

Synonym: S2166

Alternate gene names: 30063441

Gene position: 2054610-2053693 (Counterclockwise)

Preceding gene: 30063442

Following gene: 30063440

Centisome position: 44.67

GC content: 48.37

Gene sequence:

>918_bases
ATGAACTTCAGACGCCTGAAATACTTCGTAAAAATTGTAGATATTGGTAGCCTGACCCAGGCTGCTGAAGTATTGCATAT
CGCACAACCAGCGCTCAGCCAACAGGTTGCCATACTGGAAGGTGAGTTAAATCAACAGCTTTTGATTCGCACAAAACGGG
GCGTTACGCCAACAGACGCCGGAAAAATTCTCTATACCCATGCACGGGCCATTTTACGTCAGTGTGAGCAGGCCCAACTG
GCGGTGCATAACGTTGGTCAGTCATTATCGGGGCAAGTCTCGATTGGCTTTGCGCCAGGAACCGCTGCGTCATCCATCAC
CATGCCCTTATTACAGGCGGTTCGCGCTGAATTCCCGGAGGTCGTTATCTATCTTCATGAAAATAGTGGTGCAGTGCTTA
ACGAAAAACTGATAAATCACCAACTCGATATGGCGGTGATTTATGAGCATTCCCCTGTGGCTGGGGTATCCAGTCAGGCT
TTGCTGAAAGAAGATCTTTTCCTGGTAGGAACTCAAGATTGCCCGGGGCAAAACGTTGATGTTAATGCTATTGCGCAAAT
GAACCTTTTTCTCCCCAGTGATTACAGCGCTGTTAGACTTCGTGTTGATGAGGCTTTTTCCCTACGGCGGCTCACGGCAA
AAGTCATTGGTGAAATTGAGTCTATTGCCACGCTTACCGCAGCGATTGCCAGCGGCATGGGCGTTGCAGTATTACCTGAA
TCGGCTGCGCGTTCGTTATGTGGCGCAGTAAATGGGTGGATGTCACGCATTACCACTCCTTCAATGAGTCTCTCTTTGTC
CTTAAATTTACCCGCCAGAGCAAACTTGTCACCACAGGCACAGGCAGTGAAAGAGTTGTTAATGTCAGTGATTAGTTCTC
CAGTGATGGAAAAAAGGCAGTGGCAATTGGTGAGCTAA

Upstream 100 bases:

>100_bases
TAGCTTGCGCATCAACGCCAAAATAGAACTGGCAGGCATCTTGCATACTGGTTGTAAGTAATGGCGGCACTTGGGCCGAT
TCTTAAAAACCGGAGGCAAC

Downstream 100 bases:

>100_bases
GCGTTATATCGGGATGGAATAAGATGCGGGTTTTTATTATTTGTTATGCCGGGCATTAGACTTTAACAATAACGGGATAT
CTGAACTGCCCGGAGTTTAC

Product: nitrogen assimilation transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQSLSGQVSIGFAPGTAASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS

Sequences:

>Translated_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQSLSGQVSIGFAPGTAASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS
>Mature_305_residues
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQL
AVHNVGQSLSGQVSIGFAPGTAASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=305, Percent_Identity=98.3606557377049, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=30.2040816326531, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=31.3008130081301, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=27.8688524590164, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=28.968253968254, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=27.8884462151394, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=175, Percent_Identity=33.1428571428571, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787601, Length=257, Percent_Identity=26.4591439688716, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=238, Percent_Identity=22.6890756302521, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789204, Length=278, Percent_Identity=24.8201438848921, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367136, Length=254, Percent_Identity=26.3779527559055, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786202, Length=248, Percent_Identity=24.1935483870968, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787806, Length=261, Percent_Identity=24.5210727969349, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=250, Percent_Identity=26.4, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=30.0813008130081, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32863; Mature: 32863

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELNQQLLIRTKRGVTPTDA
CCHHHEEHHHHEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCC
GKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTAASSITMPLLQAVRAEFPE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCE
VVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQNVD
EEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCCC
VNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
CEEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
WQLVS
CCCCC
>Mature Secondary Structure
MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELNQQLLIRTKRGVTPTDA
CCHHHEEHHHHEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCCCC
GKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTAASSITMPLLQAVRAEFPE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCE
VVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQNVD
EEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCEEEEECCCCCCCCCC
VNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPE
CEEEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
SAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQ
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
WQLVS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]