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The map label for this gene is yneF [H]

Identifier: 30063075

GI number: 30063075

Start: 1642592

End: 1643539

Strand: Direct

Name: yneF [H]

Synonym: S1698

Alternate gene names: 30063075

Gene position: 1642592-1643539 (Clockwise)

Preceding gene: 30063074

Following gene: 30063076

Centisome position: 35.71

GC content: 50.42

Gene sequence:

>948_bases
TTGCTTGCGGACTGGTTCAGTGAGCAATTCTCAACGGGCGTGTTGATCGTGCCTTGTATGCTGACGTTGGCTATTCCTGG
AGTGCTGCCGCGCTTTAAAGCAGAACAGATGATGCCCGCCATCGCGTTTATTGTGTCGGTTATTGCCTCGGTAGTCATTG
GCGGAGCGGGGAGTCTGGCGTTCCCGCTGCCTGCATTAATCTGGTGTGCAGTGCGCTATACGCCGCAGGTAACATGTCTG
TTGACCTTTGTCACCGGTGCGGTGGAAATCGTACTGGTGGCAAATTCGGTGATTGATATCTCGGTCGGTTCGCCGTTCTC
CATTCCGGAAATGTTCTCCGCACGTCTCGGTATTGCCACGATGGCGATATGCCCAATTATGGTTTCTTTTAGCGTGGCAG
CGATCAATTTGCTAATGAAGCAAGTTGCGCTGCGAGCCGACTTTGATTTTCTGACTCAGGTTTACTCACGGTCCGGTCTT
TATGAGGCGCTGAAAAGTCCATCGCTGAAACAGACACAACATCTGACGGTCATGCTGCTTGATATCGACTATTTCAAAAG
CATTAACGATAACTATGGACATGAATGTGGCGATAAAGTGTTAAGCGTGTTTGCCCGGCATATTCAGAAGATTGTCGGTG
ATAAGGGGCTGGTGGCGCGCATGGGCGGCGAAGAATTTGCTGTTGCAGTGCCGTCGGTGAATCCTGTCGATGGTCTGCTA
ATGGCGGAAAAAATCCGTAAAGGCGTTGAACTGCAACCATTCACCTGGCAACAAAAAACGCTTTATCTCACGGTAAGTAT
TGGCGTCGGTAGTGGTCGCGCATCGTACCGAACGCTGACCGATGATTTTAATAAATTGATGGTCGAAGCCGATACGTGTC
TGTATCGCTCGAAGAAAGATGGGCGCAACCGTACCAGCACTATGCGTTACGGTGAGGAAGTTGTCTGA

Upstream 100 bases:

>100_bases
TGCTTTACGGCTATTTAATTACTGTCTGATTGCCGCATTATTATGCGCTATTGTCGGGGCGATTGGTTCGGTCAGTATTG
ATAGTCTGGATTTCTGGCCT

Downstream 100 bases:

>100_bases
CATCTTTATTCATTCCGCGCGATTTGAATGCAATGTGTTCTACCACTTTATGATCCAGGAAAAGGCGTATTTCCTCGATT
AAACCATGATCCGCGCCACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLADWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCL
LTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIATMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGL
YEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLL
MAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV

Sequences:

>Translated_315_residues
MLADWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCL
LTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIATMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGL
YEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLL
MAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV
>Mature_315_residues
MLADWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCL
LTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIATMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGL
YEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLL
MAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=315, Percent_Identity=98.7301587301587, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI87082007, Length=177, Percent_Identity=36.7231638418079, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=193, Percent_Identity=30.5699481865285, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=36.2068965517241, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=31.0975609756098, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=193, Percent_Identity=28.4974093264249, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=30.939226519337, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787816, Length=181, Percent_Identity=30.939226519337, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693134, Length=133, Percent_Identity=32.3308270676692, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=33.9506172839506, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 34427; Mature: 34427

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLADWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLA
CCCHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
FPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIAT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCEEEEECCCCCCCHHHHHHHHHHHH
MAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTVMLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEE
DIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLL
EHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHH
MAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKD
HHHHHHCCCCCCCCEECCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GRNRTSTMRYGEEVV
CCCHHHHHHCCCCCC
>Mature Secondary Structure
MLADWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLA
CCCHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
FPLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIAT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCEEEEECCCCCCCHHHHHHHHHHHH
MAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTVMLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEE
DIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLL
EHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHH
MAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTCLYRSKKD
HHHHHHCCCCCCCCEECCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GRNRTSTMRYGEEVV
CCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]