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The map label for this gene is htrB

Identifier: 30062592

GI number: 30062592

Start: 1106312

End: 1107232

Strand: Reverse

Name: htrB

Synonym: S1138

Alternate gene names: 30062592

Gene position: 1107232-1106312 (Counterclockwise)

Preceding gene: 30062594

Following gene: 30062591

Centisome position: 24.07

GC content: 52.88

Gene sequence:

>921_bases
ATGACGAATCTACCCAAGTTCTCCACCGCACTGCTTCATCCGCGTTATTGGTTAACCTGGTTGGGTATTGGCGTACTTTG
GTTGGTCGTGCAATTGCCCTACCCGATTATCTACCGCCTCGGTTGTGGATTAGGAAAACTGGCGTTACGTTTTATGAAAC
GACGCGCAAAAATTGTGCATCGCAACCTGGAACTGTGCTTCCCGGAAATGAGCGAACAAGAACGCCGTAAAATGGTGGTG
AAGAATTTCGAATCCGTTGGCATGGGCCTGATGGAAACCGGTATGGCGTGGTTCTGGCCGGACCGCCGAATCGCCCGCTG
GACGGAAGTGATCGGCATGGAACACATTCGTGACGTGCAGGCGCAAAAACGCGGCATCCTGTTAGTTGGCATCCATTTTC
TGACACTGGAGCTGGGCGCGCGGCAGTTTGGTATGCAGGAACCGGGGATTGGCGTTTACCGCCCGAACGATAATCCACTG
ATTGACTGGCTACAAACCTGGGGCCGTTTGCGCTCAAATAAATCGATGCTCGACCGCAAAGATTTAAAAGGCATGATTAA
AGCCCTGAAAAAAGGCGAAGTGGTCTGGTACGCACCAGATCATGATTACGGCCCGCGCTCAAGCGTTTTCGTCCCGTTAT
TTGCCGTTGAGCAGGCTGCGACCACGACCGGAACCTGGATGCTGGCACGGATGTCCGGCGCATGTCTGGTGCCCTTCGTT
CCACGCCGTAAGCCAGATGGCAAAGGGTATCAATTGATTATGCTGCCACCAGAGTGTTCTCCGCCACTGGATGATGCCGA
AACTACCGCCGCGTGGATGAACAAAGTGGTCGAAAAATGCATCATGATGGCACCAGAGCAGTATATGTGGTTACACCGTC
GCTTTAAAACACGCCCGGAAGGCGTTCCTTCACGCTATTAA

Upstream 100 bases:

>100_bases
GCGGCAATTTCGCCCAGTCTTCAGCCACAAATTTTGGTTGCGGGCGAAAAAATGCGACAATACATACAATTGCCCGTATA
GGTTGAAAAACAGGATTGAT

Downstream 100 bases:

>100_bases
ATCTCCCATGCCGGATGCTTCAGAATGGCATCCGGCATTACCACAGCAAATCCCCCTGATTTAGCGATAAAAGCTCTCTG
GATTGCGCCCCCTGGAAGTC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGLGKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVV
KNFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFV
PRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPEGVPSRY

Sequences:

>Translated_306_residues
MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGLGKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVV
KNFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFV
PRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPEGVPSRY
>Mature_305_residues
TNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGLGKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVK
NFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLI
DWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFVP
RRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPEGVPSRY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=99.6732026143791, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=54.485049833887, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1788161, Length=310, Percent_Identity=30.9677419354839, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35421; Mature: 35290

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGLGKLALRFMKRRAKIVH
CCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
RNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQ
HHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHH
AQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLIDWLQTWGRLRSNKSMLDRK
HHHCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
DLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHCCCEEECCC
PRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVPSRY
CCCCCC
>Mature Secondary Structure 
TNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGLGKLALRFMKRRAKIVH
CCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
RNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDRRIARWTEVIGMEHIRDVQ
HHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHH
AQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLIDWLQTWGRLRSNKSMLDRK
HHHCCEEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
DLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQAATTTGTWMLARMSGACLVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHCCCEEECCC
PRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKVVEKCIMMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVPSRY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]