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The map label for this gene is rumB

Identifier: 30062341

GI number: 30062341

Start: 840092

End: 841219

Strand: Direct

Name: rumB

Synonym: S0855

Alternate gene names: 30062341

Gene position: 840092-841219 (Clockwise)

Preceding gene: 30062340

Following gene: 30062349

Centisome position: 18.27

GC content: 54.7

Gene sequence:

>1128_bases
ATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAACGCAGCCGATTCCAGAGCAACTCTCCGC
TAAAACCGCCGATCTTAAAAATCTGCTCGCCGATTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAG
GGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGTAGCGTTGAAAAAACACTGCTCGGTATGCTGCATCGCGATGGCACG
CCGGAAGACCTTTGTGACTGCCCGCTTTATCCAGCCTCATTTGCGCCCGTTTTTGCGGCGCTAAAACCGTTTATCGCCCG
AGCGGGGTTAACGCCCTACAACGTGGCGCGTAAGCGTGGCGAACTGAAATACATTCTGCTGACTGAAAGCCAGAGTGATG
GCGGCATGATGCTGCGCTTTGTACTGCGTTCTGATACCAAGCTGGCGCAACTGCGTAAGGCGCTGCCGTGGTTACACGAA
CAACTACCGCAGCTGAAAGTTATTACCGTCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGACGGAGATCTA
CCTGACCGAACAACAGGCACTGGCGGAGCGTTTTAATGACGTACCGCTGTGGATCCGTCCGCAAAGTTTCTTCCAGACTA
ATCCGGCGGTCGCCAGCCAGTTGTACGCCACCGCGCGCGACTGGGTACGACAGCTGCCGGTTAAACATATGTGGGATCTG
TTCTGCGGTGTGGGGGGCTTTGGTTTACACTGCGCGACGCCTGACATGCAGTTAACCGGGATCGAAATTGCGCCAGAGGC
CATTGCCTGTGCAAAGCAGTCAGCCGCTGAACTGGGCTTAACTCGTTTACAATTTCAGGCGCTGGACTCCACTCAGTTTG
CCACTGCTCAGGGGGAGGTGCCGGAGCTGGTGCTGGTTAACCCGCCGCGCCGCGGCATTGGTAAACCGCTGTGTGATTAT
CTTTCAACGATGGCACCGCGTTTTATTATCTACTCCAGCTGTAACGCCCAAACCATGGCGAAAGATATCCGCGAACTGCC
AGGTTACCGCATCGAACGGGTACAGCTTTTTGATATGTTCCCGCACACCGCGCACTATGAAGTGTTGACGCTGCTGGTGA
AGCAATAA

Upstream 100 bases:

>100_bases
CTCATCCTGATGCTGCTGTTCGTTCCCTCGACCAGTCGGCGGTTCTTCCAGTTGCAATAATGTGTATAATCGTCGCCCCT
GATGATGTGAAGGTCAATGT

Downstream 100 bases:

>100_bases
AAAAGCCACAGGTGCGGCTTCAGATTGCTGATCAGGTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGC
CTACAAAATCATGCTAAATC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ

Sequences:

>Translated_375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ
>Mature_375_residues
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGT
PEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDY
LSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=369, Percent_Identity=23.8482384823848, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI51173878, Length=369, Percent_Identity=23.8482384823848, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1787083, Length=375, Percent_Identity=98.6666666666667, Blast_Score=769, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=376, Percent_Identity=27.9255319148936, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=29.6703296703297, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24666579, Length=361, Percent_Identity=24.0997229916898, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 41970; Mature: 41970

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK
CCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCE
MVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRG
EEEECCHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHEQLPQLKVITVNIQPVHMAIM
CEEEEEEECCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECEEEEEEE
EGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
CCCEEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV
HHCCCCCCEEECCCCCEEECEEECHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCC
PELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMF
CEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
PHTAHYEVLTLLVKQ
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK
CCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCE
MVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRG
EEEECCHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHEQLPQLKVITVNIQPVHMAIM
CEEEEEEECCCCCCCCEEEEEHHCCCHHHHHHHHHHHHHHHCCCEEEEEEEECEEEEEEE
EGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL
CCCEEEEEEHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHH
FCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV
HHCCCCCCEEECCCCCEEECEEECHHHHHHHHHHHHHHCHHHHHHHHCCCCCEEECCCCC
PELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDIRELPGYRIERVQLFDMF
CEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
PHTAHYEVLTLLVKQ
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA