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The map label for this gene is insF1 [C]

Identifier: 30062025

GI number: 30062025

Start: 492349

End: 492969

Strand: Reverse

Name: insF1 [C]

Synonym: S0485

Alternate gene names: 30062025

Gene position: 492969-492349 (Counterclockwise)

Preceding gene: 30062026

Following gene: 30062021

Centisome position: 10.72

GC content: 54.43

Gene sequence:

>621_bases
ATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGACCATCAGCCGGAAAGCCGTTGCCGCAGG
CGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGGTGGCTGATTTTACTTACGTCAGCACATGGC
GGGGCTTCGTCTATGTGGCGTTCATCATTGATGTGTTTGCCGGATACATCGTGGGGTGGCGGGTCTCATCGTCCATGGAA
ACGACATTCGTGCTGGATGCACTGGAGCAGGCGTTATGGGCCCGTCGACCGTCCGGCACGGTCCATCACAGTGATAAAGG
TTCTCAGTATGTATCGCTGGCCTACACACAGCGGCTTAAGGAAGCCGGATTACTGGCATCAACAGGAAGTACAGGCGACT
CGTATGACAACGCGATGGCGGAGAGCATCAATGGTCTTTACAAAGCGGAGGTAATACACCGTAAGAGCTGGAAAAACCGT
GCAGAAGTGGAACTGGCCACACTAACGTGGGTGGACTGGTATAACAATCGACGATTGCTGGAAAGGCTGGGCCACATCCC
TCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGA

Upstream 100 bases:

>100_bases
AGCGCGTATACGATGAAAATCACAAGGTATACGGTGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGTATCAGAGTGGCCA
GATGCACTGTGGCACGTCTC

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCCAGGAAACCCGGGGCGGTTCAGTTGTCGAACCAGTAGTCGAGACACTGGGCGACCGTCA
TGGAGTTCTCCACCTTCTCT

Product: IS629 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSME
TTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNR
AEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA

Sequences:

>Translated_206_residues
MAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSME
TTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNR
AEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA
>Mature_205_residues
AVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMET
TFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRA
EVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=170, Percent_Identity=54.1176470588235, Blast_Score=176, Evalue=9e-45,
Organism=Escherichia coli, GI1788405, Length=202, Percent_Identity=32.1782178217822, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787263, Length=202, Percent_Identity=32.1782178217822, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786753, Length=202, Percent_Identity=32.1782178217822, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786570, Length=202, Percent_Identity=32.1782178217822, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786490, Length=202, Percent_Identity=32.1782178217822, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789981, Length=197, Percent_Identity=28.4263959390863, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 23012; Mature: 22881

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVA
CCCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEHHHHHHHHH
EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLL
HCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEEEHHHCCHHHH
ERLGHIPPAEAEKAYYASIGNDDLAA
HHHCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
AVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVA
CCHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHH
FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEHHHHHHHHH
EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLL
HCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEEEHHHCCHHHH
ERLGHIPPAEAEKAYYASIGNDDLAA
HHHCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]