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The map label for this gene is ybbB

Identifier: 30061995

GI number: 30061995

Start: 459664

End: 460758

Strand: Reverse

Name: ybbB

Synonym: S0449

Alternate gene names: 30061995

Gene position: 460758-459664 (Counterclockwise)

Preceding gene: 30061996

Following gene: 30061993

Centisome position: 10.02

GC content: 54.61

Gene sequence:

>1095_bases
ATGCAAGAGAGACACACGGAACAGGACTATCGTGCCCTGCTGATTGCTGATACGCCCATTATTGATGTTCGCGCCCCTAT
CGAGTTTGAGCAAGGCGCAATGCCCGCCGCTATCAATCTGCCGTTAATGGATAACGATGAACGCGCCTCCGTTGGCACCT
GCTATAAACAGCAAGGCTCAGACGCAGCGCTGGCGCTGGGGCATAAACTGGTGGCGGGTGAAATTCGTCAGCAGCGTATG
TACGCCTGGCGGGCAGCGTGCCTGCAAAATCCGCAAGGTATTCTCTGCTGCGCCCGTGGCGGTCAACGTTCACATATTGT
GCAACGCTGGCTGCATGAAGCGGGGATTGATTATCCGCTGGTGGAAGGCGGTTATAAGGCACTGCGCCAGACCGCGATTC
AGGCGACCATTGAACTGGCACAAAAACCGATAGTGCTGATTGGCGGTTGTACCGGCAGCGGTAAAACGCTATTAGTGCAA
CAACAGCCAAACGGTGTTGATCTGGAAGGGCTGGCGCGTCATCGCGGTTCGGCGTTTGGTCGCACGTTACAACCACAACT
TAGCCAGGCGAGTTTTGAAAACCTGCTGGCTGCCGAAATGCTAAAAACCGACGCCCGTCAGGATTTGCGCCTGTGGGTGC
TGGAAGATGAAAGCCGGATGATCGGTTCGAATCACCTGCCGGAATGTCTGCGCGAGCGAATGACTCAGGCGGCGATTGCG
GTGGTAGAAGATCCGTTTGAGATCCGTCTTGAACGCCTGAATGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCA
CGCTTATGGCGACGAACAGGGCTGGCAGGAATATTGCGAATACCTGCATCACGGACTTTCGGCGATTAAGCGTCGGCTGG
GGTTACAGCGCTATAACGAACTCGCTGCCCAGCTGGATACGGCGCTGACAACGCAACTCACCACCGGCAGCACCGACGGT
CATCTGGCCTGGCTGGTGCCGTTACTTAAAGAATATTACGACCCAATGTATCGCTATCAGCTTGAGAAAAAAGCGGAAAA
AGTTGTCTTTCGCGGTGAGTGGGCGGAAGTGGCGGTATGGGTTAAGGCGCAGTGA

Upstream 100 bases:

>100_bases
TTTTAGAAATTTTCGGCACCCCACGCAGTTAAAAATCAAGTTTCCTTGCGCGTTGGCGCGTAAAATAGCGCGCAAGCTGA
TTTGAGGTTCACTTTTAACT

Downstream 100 bases:

>100_bases
TGGGCGGTATTCTGAAACGGCGATTGTCGTGAGAACAAAATGGTTGCACCAGAATACAGATATAAAAAAACCGACATGAC
AGTCGGTTTTTTTATTTTTT

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMDNDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRM
YAWRAACLQNPQGILCCARGGQRSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDG
HLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ

Sequences:

>Translated_364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMDNDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRM
YAWRAACLQNPQGILCCARGGQRSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDG
HLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ
>Mature_364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMDNDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRM
YAWRAACLQNPQGILCCARGGQRSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDG
HLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=364, Percent_Identity=96.7032967032967, Blast_Score=727, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 41239; Mature: 41239

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMDNDERASVGTCYKQQGS
CCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCC
DAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQRSHIVQRWLHEAGIDYPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCC
VEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQQQPNGVDLEGLARHRGSAFG
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHCCHHCC
RTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHH
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
HHCCHHHHHHHHHCHHHHEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAVW
HHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEECCCEEEEEE
VKAQ
EECC
>Mature Secondary Structure
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMDNDERASVGTCYKQQGS
CCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCC
DAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQRSHIVQRWLHEAGIDYPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCC
VEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQQQPNGVDLEGLARHRGSAFG
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHCCHHCC
RTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHH
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
HHCCHHHHHHHHHCHHHHEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYDPMYRYQLEKKAEKVVFRGEWAEVAVW
HHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEEECCCEEEEEE
VKAQ
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA