Click here to switch to the map view.

The map label for this gene is mltD

Identifier: 30061763

GI number: 30061763

Start: 224640

End: 225986

Strand: Reverse

Name: mltD

Synonym: S0205

Alternate gene names: 30061763

Gene position: 225986-224640 (Counterclockwise)

Preceding gene: 30061764

Following gene: 30061760

Centisome position: 4.91

GC content: 51.82

Gene sequence:

>1347_bases
ATGAAGGCAAAAGCGATATTACTCGCCTCTGTCCTGCTCGTGGGTTGCCAGAGTACCGGCAACGTTCAACAGCACGCACA
GAGCCTTTCTGCAGCTGGTCAAGGGGAAGCAGCAAAGTTTACAAGTCAGGCACGATGGATGGACGATGGGACGTCTATCG
CGCCAGATGGTGACTTGTGGGCTTTCATTGGCGACGAGCTAAAGATGGGAATTCCGGAAAATGACCGGATTCGCGAACAG
AAACAGAAATATTTACGCAATAAGAGCTATCTCCACGATGTAACTTTACGGGCAGAGCCGTATATGTACTGGATAGCAGG
GCAAGTTAAAAAACGTAACATGCCTATGGAACTGGTACTACTACCCATAGTGGAGAGCGCTTTTGATCCTCACGCAACGT
CTGGCGCCAATGCCGCAGGCATCTGGCAGATCATTCCGAGCACGGGGCGCAATTATGGTTTGAAACAGACCCGCAATTAT
GACGCGCGTCGCGATGTTGTTGCTTCAACAACTGCCGCGCTGAATATGATGCAGCGTCTGAACAAAATGTTTGACGACGA
CTGGCTTCTGACCGTAGCGGCTTATAACAGCGGCGAAGGTCGGGTCATGAAGGCAATTAAAACGAACAAAGCGCGTGGGA
AATCCACGGACTTCTGGTCGTTACCGTTGCCGCAGGAAACGAAGCAGTACGTGCCTAAAATGCTGGCATTGAGTGATATT
CTCAAAAACAGCAAGCGTTATGGCGTACGTCTGCCAACGACCGATGAAAGCCGTGCTCTGGCGCGTGTGCACCTGAGTAG
CCCGGTTGAAATGGCGAAGGTTGCAGATATGGCGGGGATTTCCGTCAGCAAGCTGAAGACATTCAACGCTGGCGTGAAAG
GCTCCACGCTGGGCGCAAGTGGGCCGCAGTACGTGATGGTGCCAAAGAAGCATGCAGATCAACTGCGTGAATCTCTCGCT
TCAGGCGAAATTGCTGCTGTACAGTCGACGCTGGTTGCCAACAATACGCCGCTTAACAGCCGTGTTTACACCGTACGCTC
TGGCGACACGCTTTCAAGCATCGCTTCACGTCTCGGCGTAAGCACCAAAGATTTGCAGCAGTGGAACAAACTGCGCGGAT
CTAAGCTGAAGCCAGGCCAAAGTTTGACGATTGGTGCACAGCGACTGGCAAACAACAGCGATAGCATTACGTATCGTGTG
CGCAAAGGCGATTCGCTTTCAAGCATTGCTAAACGCCACGGCGTGAACATCAAAGATGTGATGCGCTGGAACAGCGACAC
TGCGAATCTGCAACCAGGCGACAAGCTGACGTTGTTTGTGAAAAACAACAACATGCCAGATTCCTGA

Upstream 100 bases:

>100_bases
GGTTAAGGTCAAAGAAAGATAGGTTCTGATAAAACTTTCTTGTCATCGGCTCCGTTCGCCGTTATGATCGGTCGTCTTTT
AAGCAACTATTGACACACAC

Downstream 100 bases:

>100_bases
CAAACCAGATAATAAAAAGGTACCGATTCCCCCGGTGCCTTTTTTATTTATGCCGCTTTATGTGCTTCTACCATGATGAT
ATCACTGGTGAAAGAGCCGT

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQ
KQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLA
SGEIAAVQSTLVANNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANNSDSITYRV
RKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS

Sequences:

>Translated_448_residues
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQ
KQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLA
SGEIAAVQSTLVANNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANNSDSITYRV
RKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS
>Mature_448_residues
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQ
KQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLA
SGEIAAVQSTLVANNTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANNSDSITYRV
RKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=452, Percent_Identity=98.6725663716814, Blast_Score=911, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 49173; Mature: 49173

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLW
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVL
EEECCHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEEECCCEEEEEECHHHCCCCCCEEEE
LPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDVVASTTAALNMMQRL
HHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
HHHCCCCEEEEEEEEECCCCCEEEEEHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGAS
HHCCCCCCEECCCCCCCHHEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
GPQYVMVPKKHADQLRESLASGEIAAVQSTLVANNTPLNSRVYTVRSGDTLSSIASRLGV
CCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCC
STKDLQQWNKLRGSKLKPGQSLTIGAQRLANNSDSITYRVRKGDSLSSIAKRHGVNIKDV
CHHHHHHHHHHCCCCCCCCCCEEHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCHHHH
MRWNSDTANLQPGDKLTLFVKNNNMPDS
HHCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQARWMDDGTSIAPDGDLW
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEE
AFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYMYWIAGQVKKRNMPMELVL
EEECCHHCCCCCCCHHHHHHHHHHHHCCCHHEEEEEECCCEEEEEECHHHCCCCCCEEEE
LPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNYDARRDVVASTTAALNMMQRL
HHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDFWSLPLPQETKQYVPKMLALSDI
HHHCCCCEEEEEEEEECCCCCEEEEEHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHH
LKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVADMAGISVSKLKTFNAGVKGSTLGAS
HHCCCCCCEECCCCCCCHHEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
GPQYVMVPKKHADQLRESLASGEIAAVQSTLVANNTPLNSRVYTVRSGDTLSSIASRLGV
CCCEEEECHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHCC
STKDLQQWNKLRGSKLKPGQSLTIGAQRLANNSDSITYRVRKGDSLSSIAKRHGVNIKDV
CHHHHHHHHHHCCCCCCCCCCEEHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCHHHH
MRWNSDTANLQPGDKLTLFVKNNNMPDS
HHCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]