Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 30024050

GI number: 30024050

Start: 1606379

End: 1607122

Strand: Reverse

Name: trmD

Synonym: L6128

Alternate gene names: 30024050

Gene position: 1607122-1606379 (Counterclockwise)

Preceding gene: 15673547

Following gene: 15673545

Centisome position: 67.94

GC content: 37.1

Gene sequence:

>744_bases
ATGAGAATTGATATTTTAAGTATCTTTCCGGAAATGTTTGGCCCACTTAATCAGTCAATCGTAGGTAAAGCTCAAGATAA
AGGTATTTTGGAGCTACATACACATGATTTTCGAGAAAATGCAACGAATAAACAACGTCATGTTGATGATATGCCTTATG
GTGGTGGTCAAGGGATGTTATTAATGCCACAGCCAATCTTTGACACAATGGATAAAATACCTCAGAAGCCTGAAAAACCT
GCGCGTGTTATTCTTTTAGACCCAGCTGGGAAAAAATTTGACCAAAAAATGGCCGAAGAATTATCTCAAGAAGAGCAATT
AATTTTTATTTGCGGACATTATGAAGGCTACGATGAACGCATTAAAACATTGGTGACGGATGAAATTTCATTGGGAGATT
TTGTTTTAACTGGTGGAGAAGTTGCAGCAACGGTGATGGTTGATGCGGTTGTGCGTTTAATTCCAGGTGTTTTAGGAAAA
GTGGCTAGCCATGAAGATGATTCTTTTTCTAGTGGACTTTTAGAATATCCGCAATATACTCGCCCAGAAGATTTTAGAGG
GATGAAGGTTCCTGAGGTATTAATGAGTGGTCATCATGAAAATATTCGTAAATGGCGGTTAAAAGAATCTTTGAGAAAAA
CCTTAGAACGCCGTCCAGATTTATTGGATAAATATGAGCCTAATGAAGAAGAGTTAAAAATGCTACAATTATTGCGTGAA
AATGTTCAAGATGTGGTAGAATGA

Upstream 100 bases:

>100_bases
GATTTATTATTGCCTTATATTCCGCCAGTTATTTTAAACGTAGATGTGAATCAACACCGAGTGGACGTATCTATTATGGA
AGGATTGGATGACTAGAAAT

Downstream 100 bases:

>100_bases
AGTAGTGTTAAGATTTTAGTTGAATATTGGGGAATATCCTCTGAATTTGGATGATGAAATCTTTGTTCTTATTGTTCATA
TCTGGATATAACTAAATTTA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRIDILSIFPEMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGGQGMLLMPQPIFDTMDKIPQKPEKP
ARVILLDPAGKKFDQKMAEELSQEEQLIFICGHYEGYDERIKTLVTDEISLGDFVLTGGEVAATVMVDAVVRLIPGVLGK
VASHEDDSFSSGLLEYPQYTRPEDFRGMKVPEVLMSGHHENIRKWRLKESLRKTLERRPDLLDKYEPNEEELKMLQLLRE
NVQDVVE

Sequences:

>Translated_247_residues
MRIDILSIFPEMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGGQGMLLMPQPIFDTMDKIPQKPEKP
ARVILLDPAGKKFDQKMAEELSQEEQLIFICGHYEGYDERIKTLVTDEISLGDFVLTGGEVAATVMVDAVVRLIPGVLGK
VASHEDDSFSSGLLEYPQYTRPEDFRGMKVPEVLMSGHHENIRKWRLKESLRKTLERRPDLLDKYEPNEEELKMLQLLRE
NVQDVVE
>Mature_247_residues
MRIDILSIFPEMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGGQGMLLMPQPIFDTMDKIPQKPEKP
ARVILLDPAGKKFDQKMAEELSQEEQLIFICGHYEGYDERIKTLVTDEISLGDFVLTGGEVAATVMVDAVVRLIPGVLGK
VASHEDDSFSSGLLEYPQYTRPEDFRGMKVPEVLMSGHHENIRKWRLKESLRKTLERRPDLLDKYEPNEEELKMLQLLRE
NVQDVVE

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=237, Percent_Identity=47.2573839662447, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_LACLA (Q9CFB7)

Other databases:

- EMBL:   AE005176
- PIR:   D86820
- RefSeq:   NP_267720.2
- ProteinModelPortal:   Q9CFB7
- SMR:   Q9CFB7
- GeneID:   1115224
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L6128
- NMPDR:   fig|272623.1.peg.1606
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   LLAC272623:L6128-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28246; Mature: 28246

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDILSIFPEMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGGQGML
CCCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEHHHHHHHCCCCHHHCCCCCCCCCCCCEE
LMPQPIFDTMDKIPQKPEKPARVILLDPAGKKFDQKMAEELSQEEQLIFICGHYEGYDER
ECCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHH
IKTLVTDEISLGDFVLTGGEVAATVMVDAVVRLIPGVLGKVASHEDDSFSSGLLEYPQYT
HHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCC
RPEDFRGMKVPEVLMSGHHENIRKWRLKESLRKTLERRPDLLDKYEPNEEELKMLQLLRE
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHH
NVQDVVE
HHHHHHC
>Mature Secondary Structure
MRIDILSIFPEMFGPLNQSIVGKAQDKGILELHTHDFRENATNKQRHVDDMPYGGGQGML
CCCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEHHHHHHHCCCCHHHCCCCCCCCCCCCEE
LMPQPIFDTMDKIPQKPEKPARVILLDPAGKKFDQKMAEELSQEEQLIFICGHYEGYDER
ECCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHH
IKTLVTDEISLGDFVLTGGEVAATVMVDAVVRLIPGVLGKVASHEDDSFSSGLLEYPQYT
HHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCC
RPEDFRGMKVPEVLMSGHHENIRKWRLKESLRKTLERRPDLLDKYEPNEEELKMLQLLRE
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHH
NVQDVVE
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471