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The map label for this gene is dhaK

Identifier: 30023982

GI number: 30023982

Start: 244069

End: 245055

Strand: Direct

Name: dhaK

Synonym: L44063

Alternate gene names: 30023982

Gene position: 244069-245055 (Clockwise)

Preceding gene: 15672227

Following gene: 30023984

Centisome position: 10.32

GC content: 34.85

Gene sequence:

>987_bases
ATGAAATTCTATAATTCAACAAATGAAATTCCAGAGGAGATGTTAAAAGGGATTGATTTGACTTACCCTCAGTTGACTTA
TCTACCAGAAACTGGAATTCTCTATGATAATACATATAATGAAAAAACAGTTCCAATTATTTCAGGTGGAGGAAGTGGTC
ATGAACCGGCCCATGTAGGTTACGTTGGTTCTGGAATGCTTGCGGCAGCTGTGACTGGTCCTCTATTTATACCACCAAAG
TCTAAAAATATTTTAAAAGCTATTCGCCAAGTTAACTCAGGCAAAGGCGTTTTTGTGATTATCAAAAATTTTGAAGCTGA
CTTAAAGGAATTTAATGAGGCAATAAAAGAAGCGAGAACTGAAGGAATTGATGTAAGATATATTGTCAGTCATGATGATA
TTTCGGTAAATGCTTATAATTTTCATAAAAGACATCGAGGAGTTGCTGGAACAATTTTACTTCATAAAATTCTCGGTGCT
TTTGCTAAAGAAGGCGGAAGTATTGATGAAATTGAACAATTAGCCCTGTCACTTTCCCCAGAAATTTATACTTTGGGTGT
TGCCTTAGCACCTGTTCATTTTCCTCATCAAAAGACTTCATTTGTTCTAGCTGAAGATGAAGTTTCTTTTGGAATTGGAA
TACACGGAGAACCCGGTTATCGTGTTGAAAAATTTGAAGGCTCAGAGCGTATTGCCATAGAGTTAGTAAATAAGTTAAAA
GCAGAAATTAACTGGCAAAAAAAAGCAAATAAAAATTATATTTTGCTTGTAAATGGTTTGGGTTCAACAACATTAATGGA
ACTTTACAGTTTTCAATATGATGTGATGCGCCTTTTAGAACTTGAAGGCTTATCTGTTAAGTTTTGCAAAGTTGGAAACT
TAATGACAAGTTGTGATATGTCAGGAATTTCATTAACGCTTTGTTCTGTTAAAGATCCTAAATGGTTGGACTATCTGAAT
GTTCCAACGGGAGCTTTTGCATGGTAA

Upstream 100 bases:

>100_bases
CTTGCAGCTTTGACTGAAAAATAATCTAAAAGCTAATAAATAAAAAGAGTTTGATTTTTGAAATCAAGCTCTTTTTATAT
CTGTGCTATAATTTGGTTAT

Downstream 100 bases:

>100_bases
TCAGCTTTCAAAAATTTTAATTTGAGCAAGTAAAAGATTTACAATCTGAGAATAAAGCTTTTCTAAAGAATTTCCTCTAA
TGATTGATTCTCTAGTTATT

Product: DhaKLM operon coactivator DhaQ

Products: NA

Alternate protein names: DhaS coactivator dhaQ

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKFYNSTNEIPEEMLKGIDLTYPQLTYLPETGILYDNTYNEKTVPIISGGGSGHEPAHVGYVGSGMLAAAVTGPLFIPPK
SKNILKAIRQVNSGKGVFVIIKNFEADLKEFNEAIKEARTEGIDVRYIVSHDDISVNAYNFHKRHRGVAGTILLHKILGA
FAKEGGSIDEIEQLALSLSPEIYTLGVALAPVHFPHQKTSFVLAEDEVSFGIGIHGEPGYRVEKFEGSERIAIELVNKLK
AEINWQKKANKNYILLVNGLGSTTLMELYSFQYDVMRLLELEGLSVKFCKVGNLMTSCDMSGISLTLCSVKDPKWLDYLN
VPTGAFAW

Sequences:

>Translated_328_residues
MKFYNSTNEIPEEMLKGIDLTYPQLTYLPETGILYDNTYNEKTVPIISGGGSGHEPAHVGYVGSGMLAAAVTGPLFIPPK
SKNILKAIRQVNSGKGVFVIIKNFEADLKEFNEAIKEARTEGIDVRYIVSHDDISVNAYNFHKRHRGVAGTILLHKILGA
FAKEGGSIDEIEQLALSLSPEIYTLGVALAPVHFPHQKTSFVLAEDEVSFGIGIHGEPGYRVEKFEGSERIAIELVNKLK
AEINWQKKANKNYILLVNGLGSTTLMELYSFQYDVMRLLELEGLSVKFCKVGNLMTSCDMSGISLTLCSVKDPKWLDYLN
VPTGAFAW
>Mature_328_residues
MKFYNSTNEIPEEMLKGIDLTYPQLTYLPETGILYDNTYNEKTVPIISGGGSGHEPAHVGYVGSGMLAAAVTGPLFIPPK
SKNILKAIRQVNSGKGVFVIIKNFEADLKEFNEAIKEARTEGIDVRYIVSHDDISVNAYNFHKRHRGVAGTILLHKILGA
FAKEGGSIDEIEQLALSLSPEIYTLGVALAPVHFPHQKTSFVLAEDEVSFGIGIHGEPGYRVEKFEGSERIAIELVNKLK
AEINWQKKANKNYILLVNGLGSTTLMELYSFQYDVMRLLELEGLSVKFCKVGNLMTSCDMSGISLTLCSVKDPKWLDYLN
VPTGAFAW

Specific function: Coactivator for the transcription factor dhaS. The heterotetramer formed by dhaQ and dhaS functions as transcriptional regulator. Activated by covalent binding of dihydroxyacetone to dhaQ. The complex activates the dhaKLM operon

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain

Homologues:

Organism=Homo sapiens, GI20149621, Length=336, Percent_Identity=34.5238095238095, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI87081857, Length=341, Percent_Identity=33.1378299120235, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17565018, Length=285, Percent_Identity=33.6842105263158, Blast_Score=170, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6323570, Length=340, Percent_Identity=34.1176470588235, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321055, Length=343, Percent_Identity=29.4460641399417, Blast_Score=148, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHAQ_LACLA (Q9CIW0)

Other databases:

- EMBL:   AE005176
- PIR:   F86655
- RefSeq:   NP_266402.2
- PDB:   2IU4
- PDB:   2IU6
- PDBsum:   2IU4
- PDBsum:   2IU6
- ProteinModelPortal:   Q9CIW0
- SMR:   Q9CIW0
- GeneID:   1113856
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L44063
- NMPDR:   fig|272623.1.peg.254
- HOGENOM:   HBG473357
- OMA:   FEGSERI
- ProtClustDB:   PRK14483
- BioCyc:   LLAC272623:L44063-MONOMER
- GO:   GO:0006350
- InterPro:   IPR004006
- InterPro:   IPR012735
- TIGRFAMs:   TIGR02362

Pfam domain/function: PF02733 Dak1

EC number: NA

Molecular weight: Translated: 36236; Mature: 36236

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS51481 DHAK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYNSTNEIPEEMLKGIDLTYPQLTYLPETGILYDNTYNEKTVPIISGGGSGHEPAHVG
CCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCEEEECCCCCEEEEEEECCCCCCCCCEEE
YVGSGMLAAAVTGPLFIPPKSKNILKAIRQVNSGKGVFVIIKNFEADLKEFNEAIKEART
EECCCCEEHHHCCCEEECCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHH
EGIDVRYIVSHDDISVNAYNFHKRHRGVAGTILLHKILGAFAKEGGSIDEIEQLALSLSP
CCCCEEEEEECCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EIYTLGVALAPVHFPHQKTSFVLAEDEVSFGIGIHGEPGYRVEKFEGSERIAIELVNKLK
CEEEEEEEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
AEINWQKKANKNYILLVNGLGSTTLMELYSFQYDVMRLLELEGLSVKFCKVGNLMTSCDM
HHCCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCC
SGISLTLCSVKDPKWLDYLNVPTGAFAW
CCCEEEEEECCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKFYNSTNEIPEEMLKGIDLTYPQLTYLPETGILYDNTYNEKTVPIISGGGSGHEPAHVG
CCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCEEEECCCCCEEEEEEECCCCCCCCCEEE
YVGSGMLAAAVTGPLFIPPKSKNILKAIRQVNSGKGVFVIIKNFEADLKEFNEAIKEART
EECCCCEEHHHCCCEEECCCCHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHH
EGIDVRYIVSHDDISVNAYNFHKRHRGVAGTILLHKILGAFAKEGGSIDEIEQLALSLSP
CCCCEEEEEECCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EIYTLGVALAPVHFPHQKTSFVLAEDEVSFGIGIHGEPGYRVEKFEGSERIAIELVNKLK
CEEEEEEEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
AEINWQKKANKNYILLVNGLGSTTLMELYSFQYDVMRLLELEGLSVKFCKVGNLMTSCDM
HHCCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCC
SGISLTLCSVKDPKWLDYLNVPTGAFAW
CCCEEEEEECCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471