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The map label for this gene is pgk

Identifier: 15672227

GI number: 15672227

Start: 242796

End: 243992

Strand: Direct

Name: pgk

Synonym: L0010

Alternate gene names: 15672227

Gene position: 242796-243992 (Clockwise)

Preceding gene: 15672226

Following gene: 30023982

Centisome position: 10.26

GC content: 41.1

Gene sequence:

>1197_bases
ATGGCAAAATTGACTGTAAAAGACGTTGAACTTAAAGGCAAAAAAGTCCTCGTACGTGTTGACTTTAATGTTCCAATTAA
AGATGGCGTAATCACAAATGATAACCGTATCACTGCTGCGCTTCCAACAATTAAATACATCCTTGAACAAGGTGGACGTG
CAGTCCTCTTCTCTCACTTGGGACGTGTTAAAGAAGAAGCTGACAAAGCTGGTAAATCACTTGCACCAGTAGCTAAAGCT
CTTTCTGAAAAACTTGGTCAAGATGTTGTTTTCCCAGGAACTACTCGTGGCGCAGAACTTGAAGCAGCAATCAACGAACT
TAAAGATGGAGAAATCCTTCTCGTTGAAAACACTCGTTTTGAAGACATCGACGGTAAAAAAGAATCTAAAAACGACCCAG
AACTTGGTAAATACTGGGCTTCACTTGGTGACGGAATCTTCGTTAATGACGCATTTGGTACAGCTCACCGTGCTCATGCA
TCAAACGTTGGTATCTCAGCAAACGTTGATAAAGCCGTTGCTGGTTTCCTTCTTGAAAACGAAATTGCTTATATCCAAGA
AGCAGTTGAAGCTCCAGAACGTCCATTCGTAGCAATCCTTGGTGGTTCAAAAGTTTCAGATAAAATCGGTGTTATCGAAA
ACCTTCTTTCAAAAGCTGATAAAGTTATCATCGGTGGTGGGATGGCTTACACATTCTTGAAAGCTCAAGGTTACGAAATC
GGAACTTCACTCGTTGAAGATGACAAACTTGATCTTGCTAAAGAATTGCTTGAAAAAGCAGCTGGAAAATTGATTTTGCC
ACTTGACCACAAAGTTGCTAACGCATTTGCTGGTTACACAGAAGTTAAAGAAACTGCTGACCAAAATATTCCTGCAGGCT
TCATGGGACTTGACGTAGCAAGCAAAACAATTGCTGACTACAACACACAACTTGAAGGTGCTAAAACAGTTGTTTGGAAT
GGTCCAGTTGGTGTATTTGAAAACACAGACTTCCAAGCAGGTACTGTTGGATTGATGGAAGCTATCGTTAAACAACCAGG
TGTTAAATCAATCATCGGTGGTGGTGACTCAGCAGCAGCAGCAATCAACCTTGGTTATGCAGATAAATTCTCATGGATTT
CAACTGGTGGTGGAGCTTCAATGGAACTCCTCGAAGGTAAAGTACTTCCAGGACTTGCAGCTTTGACTGAAAAATAA

Upstream 100 bases:

>100_bases
TATTCGAATTTGTGATATAATTAACCTATCGATTTGAATTGAATCAGCATGGTGCTTTTTCAATCTCAACAAAAATATCT
TATAAGGAGAATTTTTCCAA

Downstream 100 bases:

>100_bases
TCTAAAAGCTAATAAATAAAAAGAGTTTGATTTTTGAAATCAAGCTCTTTTTATATCTGTGCTATAATTTGGTTATATGA
AATTCTATAATTCAACAAAT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAKLTVKDVELKGKKVLVRVDFNVPIKDGVITNDNRITAALPTIKYILEQGGRAVLFSHLGRVKEEADKAGKSLAPVAKA
LSEKLGQDVVFPGTTRGAELEAAINELKDGEILLVENTRFEDIDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
SNVGISANVDKAVAGFLLENEIAYIQEAVEAPERPFVAILGGSKVSDKIGVIENLLSKADKVIIGGGMAYTFLKAQGYEI
GTSLVEDDKLDLAKELLEKAAGKLILPLDHKVANAFAGYTEVKETADQNIPAGFMGLDVASKTIADYNTQLEGAKTVVWN
GPVGVFENTDFQAGTVGLMEAIVKQPGVKSIIGGGDSAAAAINLGYADKFSWISTGGGASMELLEGKVLPGLAALTEK

Sequences:

>Translated_398_residues
MAKLTVKDVELKGKKVLVRVDFNVPIKDGVITNDNRITAALPTIKYILEQGGRAVLFSHLGRVKEEADKAGKSLAPVAKA
LSEKLGQDVVFPGTTRGAELEAAINELKDGEILLVENTRFEDIDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
SNVGISANVDKAVAGFLLENEIAYIQEAVEAPERPFVAILGGSKVSDKIGVIENLLSKADKVIIGGGMAYTFLKAQGYEI
GTSLVEDDKLDLAKELLEKAAGKLILPLDHKVANAFAGYTEVKETADQNIPAGFMGLDVASKTIADYNTQLEGAKTVVWN
GPVGVFENTDFQAGTVGLMEAIVKQPGVKSIIGGGDSAAAAINLGYADKFSWISTGGGASMELLEGKVLPGLAALTEK
>Mature_397_residues
AKLTVKDVELKGKKVLVRVDFNVPIKDGVITNDNRITAALPTIKYILEQGGRAVLFSHLGRVKEEADKAGKSLAPVAKAL
SEKLGQDVVFPGTTRGAELEAAINELKDGEILLVENTRFEDIDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHAS
NVGISANVDKAVAGFLLENEIAYIQEAVEAPERPFVAILGGSKVSDKIGVIENLLSKADKVIIGGGMAYTFLKAQGYEIG
TSLVEDDKLDLAKELLEKAAGKLILPLDHKVANAFAGYTEVKETADQNIPAGFMGLDVASKTIADYNTQLEGAKTVVWNG
PVGVFENTDFQAGTVGLMEAIVKQPGVKSIIGGGDSAAAAINLGYADKFSWISTGGGASMELLEGKVLPGLAALTEK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=413, Percent_Identity=46.2469733656174, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=44.7941888619855, Blast_Score=319, Evalue=3e-87,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=43.21608040201, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17508823, Length=416, Percent_Identity=48.0769230769231, Blast_Score=340, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=47.4698795180723, Blast_Score=337, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=45.3012048192771, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17136394, Length=415, Percent_Identity=46.7469879518072, Blast_Score=313, Evalue=2e-85,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_LACLA (Q9CIW1)

Other databases:

- EMBL:   AE005176
- PIR:   E86655
- RefSeq:   NP_266401.1
- ProteinModelPortal:   Q9CIW1
- PhosSite:   Q9CIW1
- GeneID:   1113855
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0010
- NMPDR:   fig|272623.1.peg.253
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   LLAC272623:L0010-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42070; Mature: 41939

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 119-119 BINDING 157-157 BINDING 208-208 BINDING 296-296 BINDING 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTVKDVELKGKKVLVRVDFNVPIKDGVITNDNRITAALPTIKYILEQGGRAVLFSHL
CCCEEEEEEEECCCEEEEEEECCCCCCCCEEECCCEEEEHHHHHHHHHHCCCCEEHHHHH
GRVKEEADKAGKSLAPVAKALSEKLGQDVVFPGTTRGAELEAAINELKDGEILLVENTRF
HHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHCCCCCEEEEECCCC
EDIDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHASNVGISANVDKAVAGFLLEN
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
EIAYIQEAVEAPERPFVAILGGSKVSDKIGVIENLLSKADKVIIGGGMAYTFLKAQGYEI
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCC
GTSLVEDDKLDLAKELLEKAAGKLILPLDHKVANAFAGYTEVKETADQNIPAGFMGLDVA
CCHHHCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHH
SKTIADYNTQLEGAKTVVWNGPVGVFENTDFQAGTVGLMEAIVKQPGVKSIIGGGDSAAA
HHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCEE
AINLGYADKFSWISTGGGASMELLEGKVLPGLAALTEK
EEECCCCCCCCEEECCCCCCHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure 
AKLTVKDVELKGKKVLVRVDFNVPIKDGVITNDNRITAALPTIKYILEQGGRAVLFSHL
CCEEEEEEEECCCEEEEEEECCCCCCCCEEECCCEEEEHHHHHHHHHHCCCCEEHHHHH
GRVKEEADKAGKSLAPVAKALSEKLGQDVVFPGTTRGAELEAAINELKDGEILLVENTRF
HHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHCCCCCEEEEECCCC
EDIDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHASNVGISANVDKAVAGFLLEN
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
EIAYIQEAVEAPERPFVAILGGSKVSDKIGVIENLLSKADKVIIGGGMAYTFLKAQGYEI
HHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCC
GTSLVEDDKLDLAKELLEKAAGKLILPLDHKVANAFAGYTEVKETADQNIPAGFMGLDVA
CCHHHCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHH
SKTIADYNTQLEGAKTVVWNGPVGVFENTDFQAGTVGLMEAIVKQPGVKSIIGGGDSAAA
HHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCEE
AINLGYADKFSWISTGGGASMELLEGKVLPGLAALTEK
EEECCCCCCCCEEECCCCCCHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471