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The map label for this gene is rlmI [H]

Identifier: 29349739

GI number: 29349739

Start: 5704334

End: 5705512

Strand: Reverse

Name: rlmI [H]

Synonym: BT_4331

Alternate gene names: 29349739

Gene position: 5705512-5704334 (Counterclockwise)

Preceding gene: 29349740

Following gene: 29349738

Centisome position: 91.14

GC content: 48.01

Gene sequence:

>1179_bases
ATGCATAAAGTATACCTCAAACCCGGCAAAGAAGAGTCTTTAAAAAGATTTCATCCCTGGATTTTCTCCGGAGCAATCGC
TCGTTTCGATGGAGAACCCGAAGAAGGTGAAGTTGTAGAAGTATACACTTCGAAGAAGGAATTTATAGCCGAAGGACATT
TCCAGATCGGAAGTATCGCCGTACGTGTGCTCTCTTTCCGTCAGGAACCCATCGACCACGATTTCTGGAAACGTAAACTG
CAAATTGCCTATGATATGCGTTGTGGAATCGGTATCGCAGTCAATCCCACCAATGACACCTACCGTCTTGTACACGGTGA
GGGAGACAATCTGCCTGGGCTGGTCATTGACATTTATGCCCGGACTGCCGTCATGCAGGCGCACTCCGCAGGAATGCATG
TAGACCGCATGACTATCGCCGAAGCCCTGTCGGAAGTAATGGGTGACAAGATCGAAAATATCTATTATAAATCGGAGACT
ACACTGCCTTTTAAGGCAGATCTTTTTCCCGAAAACGGTTTCCTTAAAGGAGGAAGCAGCGATAATATCGCACGGGAATA
CGGCCTGAAGTTCCATGTAGACTGGCTGAAAGGCCAAAAGACAGGATTCTTCGTAGACCAACGCGAAAACCGTTCACTGC
TGGAGCATTATTCTAAAGACCGTTCGGTACTGAATATGTTCTGCTACACCGGCGGTTTCTCCTTCTACGCTATGCGTGGA
GGTGCAAAGCTGGTACATTCCGTTGACAGTTCTGCCAAAGCAATCGATCTGACTAACAAAAACGTAGAACTGAATTTCCC
CGGCGATGCGCGTCACGAAGCTTTCGCCGAAGATGCTTTCAAATACCTTGACCGCATGGGCGACCAATACGATTTGATAA
TCCTCGACCCGCCTGCATTTGCCAAGCATAAGGATGCTTTGCGGAATGCCTTGCAAGGTTACCGGAAACTGAATGCCAAA
GCATTCGAAAAGATCAAACCGGGCGGCATCCTGTTTACTTTCTCCTGCTCTCAGGTGGTAACCAAGGATAATTTCCGCAC
AGCTGTGTTTACCGCTGCTGCTATGTCAGGACGTAGTGTACGTATCCTGCACCAACTAACCCAGCCTGCCGACCACCCGG
TAAATATATACCATCCCGAAGGAGAATACCTGAAAGGGCTGGTACTCTATGTAGAGTAA

Upstream 100 bases:

>100_bases
CCCTGTAGAAGAAGAGGTTGACGCAAATGCTCCCGCCAATCGCTGACAAATGACCGATAAAAGACTAATTAATAACTGAT
AAAATAGCATTTTAAACAAC

Downstream 100 bases:

>100_bases
TATATGTCATAAACACCATGAACTTGCATAAACAAGGGGTGAATAAAAGAAAAATAATAGTTTCAAAGTTAATTAGAGTT
AACAAATAGAAGTTTTTCGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MHKVYLKPGKEESLKRFHPWIFSGAIARFDGEPEEGEVVEVYTSKKEFIAEGHFQIGSIAVRVLSFRQEPIDHDFWKRKL
QIAYDMRCGIGIAVNPTNDTYRLVHGEGDNLPGLVIDIYARTAVMQAHSAGMHVDRMTIAEALSEVMGDKIENIYYKSET
TLPFKADLFPENGFLKGGSSDNIAREYGLKFHVDWLKGQKTGFFVDQRENRSLLEHYSKDRSVLNMFCYTGGFSFYAMRG
GAKLVHSVDSSAKAIDLTNKNVELNFPGDARHEAFAEDAFKYLDRMGDQYDLIILDPPAFAKHKDALRNALQGYRKLNAK
AFEKIKPGGILFTFSCSQVVTKDNFRTAVFTAAAMSGRSVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE

Sequences:

>Translated_392_residues
MHKVYLKPGKEESLKRFHPWIFSGAIARFDGEPEEGEVVEVYTSKKEFIAEGHFQIGSIAVRVLSFRQEPIDHDFWKRKL
QIAYDMRCGIGIAVNPTNDTYRLVHGEGDNLPGLVIDIYARTAVMQAHSAGMHVDRMTIAEALSEVMGDKIENIYYKSET
TLPFKADLFPENGFLKGGSSDNIAREYGLKFHVDWLKGQKTGFFVDQRENRSLLEHYSKDRSVLNMFCYTGGFSFYAMRG
GAKLVHSVDSSAKAIDLTNKNVELNFPGDARHEAFAEDAFKYLDRMGDQYDLIILDPPAFAKHKDALRNALQGYRKLNAK
AFEKIKPGGILFTFSCSQVVTKDNFRTAVFTAAAMSGRSVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE
>Mature_392_residues
MHKVYLKPGKEESLKRFHPWIFSGAIARFDGEPEEGEVVEVYTSKKEFIAEGHFQIGSIAVRVLSFRQEPIDHDFWKRKL
QIAYDMRCGIGIAVNPTNDTYRLVHGEGDNLPGLVIDIYARTAVMQAHSAGMHVDRMTIAEALSEVMGDKIENIYYKSET
TLPFKADLFPENGFLKGGSSDNIAREYGLKFHVDWLKGQKTGFFVDQRENRSLLEHYSKDRSVLNMFCYTGGFSFYAMRG
GAKLVHSVDSSAKAIDLTNKNVELNFPGDARHEAFAEDAFKYLDRMGDQYDLIILDPPAFAKHKDALRNALQGYRKLNAK
AFEKIKPGGILFTFSCSQVVTKDNFRTAVFTAAAMSGRSVRILHQLTQPADHPVNIYHPEGEYLKGLVLYVE

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=397, Percent_Identity=38.2871536523929, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1787181, Length=211, Percent_Identity=27.0142180094787, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44325; Mature: 44325

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKVYLKPGKEESLKRFHPWIFSGAIARFDGEPEEGEVVEVYTSKKEFIAEGHFQIGSIA
CCEEEECCCCHHHHHHHCCHHHHCCCEECCCCCCCCCEEEEEECCHHHHHCCCEEHHHHH
VRVLSFRQEPIDHDFWKRKLQIAYDMRCGIGIAVNPTNDTYRLVHGEGDNLPGLVIDIYA
HHHHHHHCCCCCHHHHHHHEEEEEEEECCEEEEECCCCCEEEEEECCCCCCCCEEEEHHH
RTAVMQAHSAGMHVDRMTIAEALSEVMGDKIENIYYKSETTLPFKADLFPENGFLKGGSS
HHHHHHHHHCCCEEHHHHHHHHHHHHHCCHHHHEEEECCCCCCEEEECCCCCCEECCCCC
DNIAREYGLKFHVDWLKGQKTGFFVDQRENRSLLEHYSKDRSVLNMFCYTGGFSFYAMRG
CCHHHHCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECC
GAKLVHSVDSSAKAIDLTNKNVELNFPGDARHEAFAEDAFKYLDRMGDQYDLIILDPPAF
CHHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
AKHKDALRNALQGYRKLNAKAFEKIKPGGILFTFSCSQVVTKDNFRTAVFTAAAMSGRSV
HHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEECCHHHCCCCCCEEEEEHHHHCCCEE
RILHQLTQPADHPVNIYHPEGEYLKGLVLYVE
HHHHHHCCCCCCCEEEECCCCCCEEEEEEEEC
>Mature Secondary Structure
MHKVYLKPGKEESLKRFHPWIFSGAIARFDGEPEEGEVVEVYTSKKEFIAEGHFQIGSIA
CCEEEECCCCHHHHHHHCCHHHHCCCEECCCCCCCCCEEEEEECCHHHHHCCCEEHHHHH
VRVLSFRQEPIDHDFWKRKLQIAYDMRCGIGIAVNPTNDTYRLVHGEGDNLPGLVIDIYA
HHHHHHHCCCCCHHHHHHHEEEEEEEECCEEEEECCCCCEEEEEECCCCCCCCEEEEHHH
RTAVMQAHSAGMHVDRMTIAEALSEVMGDKIENIYYKSETTLPFKADLFPENGFLKGGSS
HHHHHHHHHCCCEEHHHHHHHHHHHHHCCHHHHEEEECCCCCCEEEECCCCCCEECCCCC
DNIAREYGLKFHVDWLKGQKTGFFVDQRENRSLLEHYSKDRSVLNMFCYTGGFSFYAMRG
CCHHHHCCCEEEEEEECCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECC
GAKLVHSVDSSAKAIDLTNKNVELNFPGDARHEAFAEDAFKYLDRMGDQYDLIILDPPAF
CHHHHHHCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
AKHKDALRNALQGYRKLNAKAFEKIKPGGILFTFSCSQVVTKDNFRTAVFTAAAMSGRSV
HHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEECCHHHCCCCCCEEEEEHHHHCCCEE
RILHQLTQPADHPVNIYHPEGEYLKGLVLYVE
HHHHHHCCCCCCCEEEECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA