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The map label for this gene is agaA [H]

Identifier: 29349565

GI number: 29349565

Start: 5461870

End: 5463690

Strand: Reverse

Name: agaA [H]

Synonym: BT_4157

Alternate gene names: 29349565

Gene position: 5463690-5461870 (Counterclockwise)

Preceding gene: 29349569

Following gene: 29349564

Centisome position: 87.27

GC content: 43.11

Gene sequence:

>1821_bases
ATGTATTTTTCATTTGCTAGCAAAGATAAGGGCAGTTGTAATGTCAGTACATGTAAATTCATACGTTTTCCTTTAAATTC
ATACACTTTTCATGCTTTAATCCGGTTTTTTACGCCCCAATTCTCCAAGAAACGGACGATTTTCCATCTTTCTATACTTA
TTTGCCATTGGAGCAAGTTACGACTTTGCATATCTTTGCCAAGAAAGTTATCCATTTCTACAAAAGACTACAAACGTATG
AAAAAACATCTGATTGCCTGGGGCATTTTATCTACCATGTTTATGGCAAATACTTTTGCCCAGAAAGACATCGACCGTCC
TATTATGGGATGGAGTTCATGGAACACCTATCATGTAAATATCAGTGAAGAGTTGATAAAACAGCAAGCTGACGCATTAA
TCAAACACGGACTGAAAGAGGCAGGCTACAACTATATCAATATAGATGACGGCTTCTTCGGGCATCGGGACGAAACGGGG
AAAATGCATCCGCATCCCGACCGTTTTCCCAACGGAATGAAAGTGGTTTCAGACTATATCCATTCGCTGGGATTAAAAGC
CGGAATTTACTCGGATGCAGGAGACAACACCTGCGGCTCTATCTACGACAACGACGCAAACGGAGTGGGCTCCGGACTAT
ACGGCCATGAACAGCAGGATATGGATTTATATCTTAAAGAATGGAACTATGATTTTATCAAGATCGATTATTGTGGCGGA
CGCGAACTGGGGCTGGATGAAGAAAAGCGCTACAGCACGATTTGCCAGGCTATTGCCAATACAGGAAGAACGGATGTATC
TATCAATATCTGCCGATGGGCTTTTCCCGGCACCTGGGCAAAACGACTGGCACGTTCCTGGCGTATCAGTCCTGATATCC
GTCCCAGATGGAATTCAGTAAAAGGTATTATAGAAAAGAACTTATATCTTTCCGCCTATGCTACCGACGGACATTACAAC
GATATGGATATGCTAGAAATCGGGCGTGGACTGAAACCCAACGAAGAAGAAGTCCATTTTGGAATGTGGTGCATCATGAG
TTCTCCCCTGCTGATCGGATGTGACATGAATACGATTCCCGACTTCTCATTGAAGCTACTGAAAAACAAAGAACTGATCG
CACTGAATCAGGATGTATTAGGACTTCAGGCACACGTCGTTCAACATGAAAATGAAAGCTATGTACTGGTAAAGGACATC
GAACGGAAACGCGGACTGACAAGAGCGGTAGCATTGTATAACCCTTCCGACCAGCCATGTGACTTTATCGTTCCTTTTGA
AACCCTTGAACTGGGTGGTAACGTAAAAGTACGAGACTTAATCAAACAGAAGGATCTTGGAAAAATGAAAGAAGAAATAC
GGCAGACTGTTCAGCCACACAGCGTAATGATATGCAAAATGGAAGCTGAAAAACGACTGGAACCCGTATCTTATGAGGCT
GAATGGGCATATCTTCCCTGCTACGACGATCTGGGAAAGAAATCCAAACCAATTGTTTATGTACCTGCCTCCGATTGTTC
CGGCAGAATGAAAATATCCCGACTCGGTGGTCGGGAAGAGAACTTTGCCGAATGGAGCGAAGTATACAGCGAAAAGGGAG
GTAACTATGAAATGACTATTTTCTACTCTTGCGACAAAAACCGCAAACTGGAAGTTTCTGTCAATGGGACGAAAACGGTC
CTTAAAGATTTGAACAGCAACAATGAAGTGAAATCTGTGACGATCCCTGTCAGCTTAAAACAAGGTTACAATACGGTGCG
AATGGGAAATAACTTCGGATGGGCACCGGACATCGACCGTTTTACAGTATCCAGACAATAA

Upstream 100 bases:

>100_bases
GTTCGTCAGTAAGCGCTATACCAGTCTGTGCTTTCCCATCCAAAACGGACAACGCACAAACGATTAACAATAAAAAGATT
TTTAAATGTGTCATCACTTT

Downstream 100 bases:

>100_bases
ATTCCTCATAATATCAATTATAGAATGAACAAGAAATTTAGATGCTTACTGACATCTCTGATTTTCACAGCTATGTGGAA
TGGGGCTACCATACAAGCAC

Product: alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MYFSFASKDKGSCNVSTCKFIRFPLNSYTFHALIRFFTPQFSKKRTIFHLSILICHWSKLRLCISLPRKLSISTKDYKRM
KKHLIAWGILSTMFMANTFAQKDIDRPIMGWSSWNTYHVNISEELIKQQADALIKHGLKEAGYNYINIDDGFFGHRDETG
KMHPHPDRFPNGMKVVSDYIHSLGLKAGIYSDAGDNTCGSIYDNDANGVGSGLYGHEQQDMDLYLKEWNYDFIKIDYCGG
RELGLDEEKRYSTICQAIANTGRTDVSINICRWAFPGTWAKRLARSWRISPDIRPRWNSVKGIIEKNLYLSAYATDGHYN
DMDMLEIGRGLKPNEEEVHFGMWCIMSSPLLIGCDMNTIPDFSLKLLKNKELIALNQDVLGLQAHVVQHENESYVLVKDI
ERKRGLTRAVALYNPSDQPCDFIVPFETLELGGNVKVRDLIKQKDLGKMKEEIRQTVQPHSVMICKMEAEKRLEPVSYEA
EWAYLPCYDDLGKKSKPIVYVPASDCSGRMKISRLGGREENFAEWSEVYSEKGGNYEMTIFYSCDKNRKLEVSVNGTKTV
LKDLNSNNEVKSVTIPVSLKQGYNTVRMGNNFGWAPDIDRFTVSRQ

Sequences:

>Translated_606_residues
MYFSFASKDKGSCNVSTCKFIRFPLNSYTFHALIRFFTPQFSKKRTIFHLSILICHWSKLRLCISLPRKLSISTKDYKRM
KKHLIAWGILSTMFMANTFAQKDIDRPIMGWSSWNTYHVNISEELIKQQADALIKHGLKEAGYNYINIDDGFFGHRDETG
KMHPHPDRFPNGMKVVSDYIHSLGLKAGIYSDAGDNTCGSIYDNDANGVGSGLYGHEQQDMDLYLKEWNYDFIKIDYCGG
RELGLDEEKRYSTICQAIANTGRTDVSINICRWAFPGTWAKRLARSWRISPDIRPRWNSVKGIIEKNLYLSAYATDGHYN
DMDMLEIGRGLKPNEEEVHFGMWCIMSSPLLIGCDMNTIPDFSLKLLKNKELIALNQDVLGLQAHVVQHENESYVLVKDI
ERKRGLTRAVALYNPSDQPCDFIVPFETLELGGNVKVRDLIKQKDLGKMKEEIRQTVQPHSVMICKMEAEKRLEPVSYEA
EWAYLPCYDDLGKKSKPIVYVPASDCSGRMKISRLGGREENFAEWSEVYSEKGGNYEMTIFYSCDKNRKLEVSVNGTKTV
LKDLNSNNEVKSVTIPVSLKQGYNTVRMGNNFGWAPDIDRFTVSRQ
>Mature_606_residues
MYFSFASKDKGSCNVSTCKFIRFPLNSYTFHALIRFFTPQFSKKRTIFHLSILICHWSKLRLCISLPRKLSISTKDYKRM
KKHLIAWGILSTMFMANTFAQKDIDRPIMGWSSWNTYHVNISEELIKQQADALIKHGLKEAGYNYINIDDGFFGHRDETG
KMHPHPDRFPNGMKVVSDYIHSLGLKAGIYSDAGDNTCGSIYDNDANGVGSGLYGHEQQDMDLYLKEWNYDFIKIDYCGG
RELGLDEEKRYSTICQAIANTGRTDVSINICRWAFPGTWAKRLARSWRISPDIRPRWNSVKGIIEKNLYLSAYATDGHYN
DMDMLEIGRGLKPNEEEVHFGMWCIMSSPLLIGCDMNTIPDFSLKLLKNKELIALNQDVLGLQAHVVQHENESYVLVKDI
ERKRGLTRAVALYNPSDQPCDFIVPFETLELGGNVKVRDLIKQKDLGKMKEEIRQTVQPHSVMICKMEAEKRLEPVSYEA
EWAYLPCYDDLGKKSKPIVYVPASDCSGRMKISRLGGREENFAEWSEVYSEKGGNYEMTIFYSCDKNRKLEVSVNGTKTV
LKDLNSNNEVKSVTIPVSLKQGYNTVRMGNNFGWAPDIDRFTVSRQ

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4557781, Length=425, Percent_Identity=29.8823529411765, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI4504009, Length=420, Percent_Identity=29.2857142857143, Blast_Score=161, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17562946, Length=332, Percent_Identity=30.1204819277108, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24583253, Length=418, Percent_Identity=31.8181818181818, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI19922382, Length=400, Percent_Identity=29.75, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24654142, Length=400, Percent_Identity=29.75, Blast_Score=145, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 69419; Mature: 69419

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFSFASKDKGSCNVSTCKFIRFPLNSYTFHALIRFFTPQFSKKRTIFHLSILICHWSKL
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHH
RLCISLPRKLSISTKDYKRMKKHLIAWGILSTMFMANTFAQKDIDRPIMGWSSWNTYHVN
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
ISEELIKQQADALIKHGLKEAGYNYINIDDGFFGHRDETGKMHPHPDRFPNGMKVVSDYI
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HSLGLKAGIYSDAGDNTCGSIYDNDANGVGSGLYGHEQQDMDLYLKEWNYDFIKIDYCGG
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCC
RELGLDEEKRYSTICQAIANTGRTDVSINICRWAFPGTWAKRLARSWRISPDIRPRWNSV
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHH
KGIIEKNLYLSAYATDGHYNDMDMLEIGRGLKPNEEEVHFGMWCIMSSPLLIGCDMNTIP
HHHHHCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCEEEECCCCCCC
DFSLKLLKNKELIALNQDVLGLQAHVVQHENESYVLVKDIERKRGLTRAVALYNPSDQPC
CCHHHHHCCCCEEEECCCHHCEEEEEEEECCCCEEEEEECHHHCCCEEEEEEECCCCCCC
DFIVPFETLELGGNVKVRDLIKQKDLGKMKEEIRQTVQPHSVMICKMEAEKRLEPVSYEA
EEEECHHHEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCCCC
EWAYLPCYDDLGKKSKPIVYVPASDCSGRMKISRLGGREENFAEWSEVYSEKGGNYEMTI
CEEEECCHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEE
FYSCDKNRKLEVSVNGTKTVLKDLNSNNEVKSVTIPVSLKQGYNTVRMGNNFGWAPDIDR
EEECCCCCEEEEEECCHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEECCCCCCCCCCCC
FTVSRQ
EEECCC
>Mature Secondary Structure
MYFSFASKDKGSCNVSTCKFIRFPLNSYTFHALIRFFTPQFSKKRTIFHLSILICHWSKL
CCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHH
RLCISLPRKLSISTKDYKRMKKHLIAWGILSTMFMANTFAQKDIDRPIMGWSSWNTYHVN
HHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
ISEELIKQQADALIKHGLKEAGYNYINIDDGFFGHRDETGKMHPHPDRFPNGMKVVSDYI
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HSLGLKAGIYSDAGDNTCGSIYDNDANGVGSGLYGHEQQDMDLYLKEWNYDFIKIDYCGG
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCC
RELGLDEEKRYSTICQAIANTGRTDVSINICRWAFPGTWAKRLARSWRISPDIRPRWNSV
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHH
KGIIEKNLYLSAYATDGHYNDMDMLEIGRGLKPNEEEVHFGMWCIMSSPLLIGCDMNTIP
HHHHHCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCEEEECCCCCCC
DFSLKLLKNKELIALNQDVLGLQAHVVQHENESYVLVKDIERKRGLTRAVALYNPSDQPC
CCHHHHHCCCCEEEECCCHHCEEEEEEEECCCCEEEEEECHHHCCCEEEEEEECCCCCCC
DFIVPFETLELGGNVKVRDLIKQKDLGKMKEEIRQTVQPHSVMICKMEAEKRLEPVSYEA
EEEECHHHEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHCCCCCCCCC
EWAYLPCYDDLGKKSKPIVYVPASDCSGRMKISRLGGREENFAEWSEVYSEKGGNYEMTI
CEEEECCHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEE
FYSCDKNRKLEVSVNGTKTVLKDLNSNNEVKSVTIPVSLKQGYNTVRMGNNFGWAPDIDR
EEECCCCCEEEEEECCHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEECCCCCCCCCCCC
FTVSRQ
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA