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The map label for this gene is rhgT [H]

Identifier: 29349558

GI number: 29349558

Start: 5447743

End: 5448981

Strand: Reverse

Name: rhgT [H]

Synonym: BT_4150

Alternate gene names: 29349558

Gene position: 5448981-5447743 (Counterclockwise)

Preceding gene: 29349559

Following gene: 29349557

Centisome position: 87.04

GC content: 51.41

Gene sequence:

>1239_bases
ATGAAAACAACATTTCTCGGACTTCTGCTCCTGACAGCAACAGCTATCAGCGCTCAGGAACAAGCCCGCACATTCCAACT
GGCAGACGCCCCCCGCTACAGCGAGGAAACAGGATACGGATATGACCTTGCACCCACTCCCGAAAAAGGGAGCAAAGCAC
CTTTCTTCTTTTCTGTCCGTGTGCCGGACGGTAACTACAAAGTAACCGTACGCCTCGGAAGCAAGAAGCAGGCAGGTGTA
ACTACCGTACGAGGCGAATCACGCCGTCTCTTTGTCGATAATCTGCCCACCAGAAAAGGGCAGTTCACAGAGGAGACATT
TATCATCAACAAACGGAATCCACGCATATCCGACAAAGAATCGGTACGCATCAAACCCCGTGAAAAGACGAAACTGAACT
GGGACGACAAACTGACACTGGAATTCAACGGTGACGCTCCCCTATGTCAGAGCATCAGTATCGAGCCTGCCGACCCGTCT
GTAATTACCGTATTCCTGTGCGGCAACAGTACGGTAGTCGATCAGGACAACGAGCCTTGGGCCAGCTGGGGACAAATGAT
TCCTCACTTCTTCGGCACCGATGTCTGTATCGCCAATTATGCCGAATCCGGCGAATCGGCAAATACCTTTATCGGAGCCA
AACGACTGGCGAAGGCTTTAAGCCAGATCAAAAAAGGAGACTATCTCTTTATGGAGTTCGGTCACAACGACCAAAAGCAG
AAAGGGCCGGGCAAAGGAGCTTACTACTCCTTCATGACCAGTCTGAAAACATTCATTGACGAAGCGCGTGCACGCGGAGC
GCATCCGGTGCTTGTCACTCCTACCCAACGTCGTAGTTTCGACGCCAACGGACATATCCGCGACACACACGAAGACTATC
CGGAAGCGATGCGCTGGCTGGCAGCCAAAGAGAATGTTCCCCTGATCGATCTCAATGAAATGACACGTACCCTGTACGAG
GCGCTGGGACCGGACACTTCCAAACGTGCTTTCGTCCACTATCCGGCAGGAACGTATCCGGGACAGACCAGGAATTTTGC
AGATAATACGCACTTTAATCCTTACGGTGCCTATCAGATCGCCCAATGCGTCATCGAAGGGATGAAGAAAGCCGTTCCCG
AACTGGCGAAGCATCTGAAAATAGACCCGGCATACAATCCCGCACATCCGGACGATGTGAATACTTTCCACTGGAACGAT
TCACCGTTCACGGAAATAGAGAAACCGGATGGAAATTAA

Upstream 100 bases:

>100_bases
AGGAACTATTCACCAAAGTGGCGGGAGACGTGAAATCCTATACCAAAGATTCACCCGACGAACTAAAACCGAAAATTAAT
TAAACTTATTAACGACTGAT

Downstream 100 bases:

>100_bases
AAGAATCCTATACTAAAAGACTATATGAGAATTAAAAACCTCCTTCCCCTACTTCTGGCAGGCTCGTTGATGATAGCGTG
CACTCCTGCCAAACAAGCCG

Product: putative rhamnogalacturonan acetylesterase

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKTTFLGLLLLTATAISAQEQARTFQLADAPRYSEETGYGYDLAPTPEKGSKAPFFFSVRVPDGNYKVTVRLGSKKQAGV
TTVRGESRRLFVDNLPTRKGQFTEETFIINKRNPRISDKESVRIKPREKTKLNWDDKLTLEFNGDAPLCQSISIEPADPS
VITVFLCGNSTVVDQDNEPWASWGQMIPHFFGTDVCIANYAESGESANTFIGAKRLAKALSQIKKGDYLFMEFGHNDQKQ
KGPGKGAYYSFMTSLKTFIDEARARGAHPVLVTPTQRRSFDANGHIRDTHEDYPEAMRWLAAKENVPLIDLNEMTRTLYE
ALGPDTSKRAFVHYPAGTYPGQTRNFADNTHFNPYGAYQIAQCVIEGMKKAVPELAKHLKIDPAYNPAHPDDVNTFHWND
SPFTEIEKPDGN

Sequences:

>Translated_412_residues
MKTTFLGLLLLTATAISAQEQARTFQLADAPRYSEETGYGYDLAPTPEKGSKAPFFFSVRVPDGNYKVTVRLGSKKQAGV
TTVRGESRRLFVDNLPTRKGQFTEETFIINKRNPRISDKESVRIKPREKTKLNWDDKLTLEFNGDAPLCQSISIEPADPS
VITVFLCGNSTVVDQDNEPWASWGQMIPHFFGTDVCIANYAESGESANTFIGAKRLAKALSQIKKGDYLFMEFGHNDQKQ
KGPGKGAYYSFMTSLKTFIDEARARGAHPVLVTPTQRRSFDANGHIRDTHEDYPEAMRWLAAKENVPLIDLNEMTRTLYE
ALGPDTSKRAFVHYPAGTYPGQTRNFADNTHFNPYGAYQIAQCVIEGMKKAVPELAKHLKIDPAYNPAHPDDVNTFHWND
SPFTEIEKPDGN
>Mature_412_residues
MKTTFLGLLLLTATAISAQEQARTFQLADAPRYSEETGYGYDLAPTPEKGSKAPFFFSVRVPDGNYKVTVRLGSKKQAGV
TTVRGESRRLFVDNLPTRKGQFTEETFIINKRNPRISDKESVRIKPREKTKLNWDDKLTLEFNGDAPLCQSISIEPADPS
VITVFLCGNSTVVDQDNEPWASWGQMIPHFFGTDVCIANYAESGESANTFIGAKRLAKALSQIKKGDYLFMEFGHNDQKQ
KGPGKGAYYSFMTSLKTFIDEARARGAHPVLVTPTQRRSFDANGHIRDTHEDYPEAMRWLAAKENVPLIDLNEMTRTLYE
ALGPDTSKRAFVHYPAGTYPGQTRNFADNTHFNPYGAYQIAQCVIEGMKKAVPELAKHLKIDPAYNPAHPDDVNTFHWND
SPFTEIEKPDGN

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 46175; Mature: 46175

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTFLGLLLLTATAISAQEQARTFQLADAPRYSEETGYGYDLAPTPEKGSKAPFFFSVR
CCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
VPDGNYKVTVRLGSKKQAGVTTVRGESRRLFVDNLPTRKGQFTEETFIINKRNPRISDKE
ECCCCEEEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCC
SVRIKPREKTKLNWDDKLTLEFNGDAPLCQSISIEPADPSVITVFLCGNSTVVDQDNEPW
CEEECCCCCCCCCCCCEEEEEECCCCCCHHCCCCCCCCCCEEEEEEECCCEEECCCCCCH
ASWGQMIPHFFGTDVCIANYAESGESANTFIGAKRLAKALSQIKKGDYLFMEFGHNDQKQ
HHHHHHHHHHHCCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
KGPGKGAYYSFMTSLKTFIDEARARGAHPVLVTPTQRRSFDANGHIRDTHEDYPEAMRWL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
AAKENVPLIDLNEMTRTLYEALGPDTSKRAFVHYPAGTYPGQTRNFADNTHFNPYGAYQI
HHCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHH
AQCVIEGMKKAVPELAKHLKIDPAYNPAHPDDVNTFHWNDSPFTEIEKPDGN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTTFLGLLLLTATAISAQEQARTFQLADAPRYSEETGYGYDLAPTPEKGSKAPFFFSVR
CCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
VPDGNYKVTVRLGSKKQAGVTTVRGESRRLFVDNLPTRKGQFTEETFIINKRNPRISDKE
ECCCCEEEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCC
SVRIKPREKTKLNWDDKLTLEFNGDAPLCQSISIEPADPSVITVFLCGNSTVVDQDNEPW
CEEECCCCCCCCCCCCEEEEEECCCCCCHHCCCCCCCCCCEEEEEEECCCEEECCCCCCH
ASWGQMIPHFFGTDVCIANYAESGESANTFIGAKRLAKALSQIKKGDYLFMEFGHNDQKQ
HHHHHHHHHHHCCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
KGPGKGAYYSFMTSLKTFIDEARARGAHPVLVTPTQRRSFDANGHIRDTHEDYPEAMRWL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
AAKENVPLIDLNEMTRTLYEALGPDTSKRAFVHYPAGTYPGQTRNFADNTHFNPYGAYQI
HHCCCCCEEEHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHH
AQCVIEGMKKAVPELAKHLKIDPAYNPAHPDDVNTFHWNDSPFTEIEKPDGN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]