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The map label for this gene is pehX [H]

Identifier: 29349557

GI number: 29349557

Start: 5446660

End: 5447718

Strand: Reverse

Name: pehX [H]

Synonym: BT_4149

Alternate gene names: 29349557

Gene position: 5447718-5446660 (Counterclockwise)

Preceding gene: 29349558

Following gene: 29349556

Centisome position: 87.02

GC content: 52.69

Gene sequence:

>1059_bases
ATGAGAATTAAAAACCTCCTTCCCCTACTTCTGGCAGGCTCGTTGATGATAGCGTGCACTCCTGCCAAACAAGCCGGCAA
CAGCTTCGAATGGGGGCAGGTACCTCAGCAACCGGATTTATCGTGGGCAGACAGCGTAGGCAGCCGCCAACTTCCCGGAG
ACAAGATGATTATATCCGCCAATTCCTTTGGCGCAGTAGCAGACAGTACCGTACTCAGCACAGAGGCCATTCAGAAAGCA
ATCGACAGTTGCGCTGTCTCCGGCGGAGGAACGGTGGTCCTGCAACCGGGGTATTACCAGACAGGAGCCTTATTTGTCAA
AAGCGGTGTAAACCTGCAGATTGGCAAAGGGGTAACTCTGCTTGCCAGCCCCGACATCCACCATTATCCCGAATTTCGTT
CGCGTATAGCAGGGATAGAGATGACATGGCCGGCAGCAGTGATCAACATTGTCGACGAGAAGAACGCCGCCATCAGCGGA
GAAGGTACACTGGACTGCCGTGGAAAAGTCTTCTGGGATAAATACTGGGAAATGAGGAAAGAATACGAAGCCAGAGGGTT
ACGCTGGATAGTGGATTACGACTGCAAACGTGTACGGGGAATCCTCGTCGAACGCAGTTCGGACATCACACTGAGCGGTT
TCACCCTGATGCGCACGGGATTCTGGGGATGTCAGATTCTCTATTCCGACTATTGCACCATAGACGGACTGACTATCAAC
AATAACATCGGCGGCCACGGTCCAAGCACGGACGGCATCGACATCGACTCCTCCTGCAACATACTGATCGAGAACTGTGA
CGTAGACTGCAACGACGACAATATTTGTATCAAATCGGGACGGGACGCGGACGGACTACGTGTGAACCGTCCGACAGAGA
ATGTGGTAGTGCGCAACTGCACAGCCCGCAAAGGAGCAGGACTGATTACCTGCGGCAGCGAAACTTCCGGCAGCATCCGC
AATGTATTAGGCTATGATCTTAAAGCTGTCGGCACGTATACCGTGCTGCGACTGAAAAGTGCCATGAACGTGGGGGACCC
ATTGAGAATATCTACATGA

Upstream 100 bases:

>100_bases
ACATCCGGACGATGTGAATACTTTCCACTGGAACGATTCACCGTTCACGGAAATAGAGAAACCGGATGGAAATTAAAAGA
ATCCTATACTAAAAGACTAT

Downstream 100 bases:

>100_bases
CCCGTGTCAGCGCAGAAAATGTCCGTCAGGTATTGGCGGCAGACTTAAACTGGAATCCAAGTTACAGCTACAGCACTTTG
CCCAAAGAATATGAAGGCAA

Product: exo-poly-alpha-D-galacturonosidase

Products: NA

Alternate protein names: Exo-PG [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MRIKNLLPLLLAGSLMIACTPAKQAGNSFEWGQVPQQPDLSWADSVGSRQLPGDKMIISANSFGAVADSTVLSTEAIQKA
IDSCAVSGGGTVVLQPGYYQTGALFVKSGVNLQIGKGVTLLASPDIHHYPEFRSRIAGIEMTWPAAVINIVDEKNAAISG
EGTLDCRGKVFWDKYWEMRKEYEARGLRWIVDYDCKRVRGILVERSSDITLSGFTLMRTGFWGCQILYSDYCTIDGLTIN
NNIGGHGPSTDGIDIDSSCNILIENCDVDCNDDNICIKSGRDADGLRVNRPTENVVVRNCTARKGAGLITCGSETSGSIR
NVLGYDLKAVGTYTVLRLKSAMNVGDPLRIST

Sequences:

>Translated_352_residues
MRIKNLLPLLLAGSLMIACTPAKQAGNSFEWGQVPQQPDLSWADSVGSRQLPGDKMIISANSFGAVADSTVLSTEAIQKA
IDSCAVSGGGTVVLQPGYYQTGALFVKSGVNLQIGKGVTLLASPDIHHYPEFRSRIAGIEMTWPAAVINIVDEKNAAISG
EGTLDCRGKVFWDKYWEMRKEYEARGLRWIVDYDCKRVRGILVERSSDITLSGFTLMRTGFWGCQILYSDYCTIDGLTIN
NNIGGHGPSTDGIDIDSSCNILIENCDVDCNDDNICIKSGRDADGLRVNRPTENVVVRNCTARKGAGLITCGSETSGSIR
NVLGYDLKAVGTYTVLRLKSAMNVGDPLRIST
>Mature_352_residues
MRIKNLLPLLLAGSLMIACTPAKQAGNSFEWGQVPQQPDLSWADSVGSRQLPGDKMIISANSFGAVADSTVLSTEAIQKA
IDSCAVSGGGTVVLQPGYYQTGALFVKSGVNLQIGKGVTLLASPDIHHYPEFRSRIAGIEMTWPAAVINIVDEKNAAISG
EGTLDCRGKVFWDKYWEMRKEYEARGLRWIVDYDCKRVRGILVERSSDITLSGFTLMRTGFWGCQILYSDYCTIDGLTIN
NNIGGHGPSTDGIDIDSSCNILIENCDVDCNDDNICIKSGRDADGLRVNRPTENVVVRNCTARKGAGLITCGSETSGSIR
NVLGYDLKAVGTYTVLRLKSAMNVGDPLRIST

Specific function: Contributes significantly to bacterial utilization of polygalacturonate and the induction of pectate lyase in the presence of extracellular pectic polymers [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR000743
- InterPro:   IPR013783
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00041 fn3; PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.82 [H]

Molecular weight: Translated: 38074; Mature: 38074

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKNLLPLLLAGSLMIACTPAKQAGNSFEWGQVPQQPDLSWADSVGSRQLPGDKMIISA
CCHHHHHHHHHCCCEEEEEECHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEE
NSFGAVADSTVLSTEAIQKAIDSCAVSGGGTVVLQPGYYQTGALFVKSGVNLQIGKGVTL
CCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEEEECCCEEEECCCEEE
LASPDIHHYPEFRSRIAGIEMTWPAAVINIVDEKNAAISGEGTLDCRGKVFWDKYWEMRK
EECCCCCCCHHHHHHHCCEEEECCEEEEEEECCCCCEECCCCCEEECCEEEHHHHHHHHH
EYEARGLRWIVDYDCKRVRGILVERSSDITLSGFTLMRTGFWGCQILYSDYCTIDGLTIN
HHHHCCEEEEECCCHHHHEEEEEECCCCEEEECEEEECCCCCEEEEEECCCEEECCEEEC
NNIGGHGPSTDGIDIDSSCNILIENCDVDCNDDNICIKSGRDADGLRVNRPTENVVVRNC
CCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCEEEECCCCCCCEEECCCCCCEEEEEC
TARKGAGLITCGSETSGSIRNVLGYDLKAVGTYTVLRLKSAMNVGDPLRIST
CCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHEEEEEEHHHCCCCCCEEECC
>Mature Secondary Structure
MRIKNLLPLLLAGSLMIACTPAKQAGNSFEWGQVPQQPDLSWADSVGSRQLPGDKMIISA
CCHHHHHHHHHCCCEEEEEECHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEEEE
NSFGAVADSTVLSTEAIQKAIDSCAVSGGGTVVLQPGYYQTGALFVKSGVNLQIGKGVTL
CCCCCCCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCEEEEECCCEEEECCCEEE
LASPDIHHYPEFRSRIAGIEMTWPAAVINIVDEKNAAISGEGTLDCRGKVFWDKYWEMRK
EECCCCCCCHHHHHHHCCEEEECCEEEEEEECCCCCEECCCCCEEECCEEEHHHHHHHHH
EYEARGLRWIVDYDCKRVRGILVERSSDITLSGFTLMRTGFWGCQILYSDYCTIDGLTIN
HHHHCCEEEEECCCHHHHEEEEEECCCCEEEECEEEECCCCCEEEEEECCCEEECCEEEC
NNIGGHGPSTDGIDIDSSCNILIENCDVDCNDDNICIKSGRDADGLRVNRPTENVVVRNC
CCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCEEEECCCCCCCEEECCCCCCEEEEEC
TARKGAGLITCGSETSGSIRNVLGYDLKAVGTYTVLRLKSAMNVGDPLRIST
CCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHEEEEEEHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2168372; 1409594 [H]