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The map label for this gene is tnpA [H]

Identifier: 29349552

GI number: 29349552

Start: 5439737

End: 5440846

Strand: Reverse

Name: tnpA [H]

Synonym: BT_4144

Alternate gene names: 29349552

Gene position: 5440846-5439737 (Counterclockwise)

Preceding gene: 29349553

Following gene: 29349544

Centisome position: 86.91

GC content: 42.16

Gene sequence:

>1110_bases
ATGAAGAAAAAAACAGCCAGTCCCGGCATTGTCGGTCAAAAGCAATGTGGTACTTTTTTGCATCCTGTCTTGGTAGTGGA
TGCCTCCAGTCATATTCCTATAGGCTTTTCTTCGGTTAAGCAGTGGAATCGCTCACCGGCTGCTTTAAGTCGTGAAGAAC
GTAATTACAGATATCAGCCTATAGAAGAAAAAGAGTCATATCGTTGGATAGAAAGTGGAATGGCTGCCAGTGAGCAAATG
CCCCGGGATGCAGTTAAAACGATCATCGGTGACCGTGAAGCGGACATTTTCGAGCTTTTCAGCCGTATCCCTACTGATAA
TGTTCACTTGCTGATACGTTCTGTTCATGAAAGGAATTGCCGGTTGGATGATCCAGACTGTTCTGTCCATCTGAATACAT
TAATGGAGCAGGCTGTTCTACGGGCAGAGTATAGCTTTGAAGTGCTCCCGGGAAGCGGACGTAAGAAACGGGTAGCGTGC
ATGGAACTTCGCTTTGAAAGAGTCACCTTGTGCGCTCCTGTTAACGGTCCGGCAAAGGGCAGTCCCCCTGTTAGTCTTTA
TTGCATACATGTTAAAGAAAAATCTTCCAGTACACCGGTAAATGAGAGCCCTATTGAATGGAGACTGCTAACTACACATG
TGGTGGAGACTGTAGAACAAGCAATTGAATGTATCGGTTGGTATCGTTGTAGATGGCTGATTGAAGAGTTGTTCAGAGTG
CTCAAAAGAAAGGGATTCATGATTGAGGACGCACAGTTGGAAACAGTTTCGGCATTACAAAAACTAATCTTAATTTCCTT
GCAGGCAGCCCTGCAGGTGATGGTACTCAAACTTTCTTTTGATAAAGAAGATGAAAAACTCTCTTCAGAAATCTACTTTA
CAAGCAAAGAAATAGCATTATTACATATAGTAGGAAAAAAGAGTGAGGGAAATACAAAAATACAGCAAAATCCATATAAA
AAAGAATCAATGGCATGGGCAGCATGGATTATTGCAAGGTTAGGAGCATGGAGTGCATACAAGAGCCAGTCCATTCCAGG
ATATATTACCTTTAAGAATGGACTGGATAGATTTTATACACAGTTTGAACTGTATGAGTTAATCAGCTAA

Upstream 100 bases:

>100_bases
AGGTCTGATACACACTTGCTGTAAGAATGCTTCCGGTCGTCAGCATTTACTGTGTATTCAAGATACCTCGGAGATAAACT
ATGAGGCTCATGTTGAGCGA

Downstream 100 bases:

>100_bases
GACTTGTGTATAAAGGGTAGCTTCCCGAAGGAGGAGAGTTGTGATAGAACCGACTTTTTAGTCAGCCTCTTTTCAGCCTG
TTATTTTAATCTATACTTGT

Product: transposase

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKKKTASPGIVGQKQCGTFLHPVLVVDASSHIPIGFSSVKQWNRSPAALSREERNYRYQPIEEKESYRWIESGMAASEQM
PRDAVKTIIGDREADIFELFSRIPTDNVHLLIRSVHERNCRLDDPDCSVHLNTLMEQAVLRAEYSFEVLPGSGRKKRVAC
MELRFERVTLCAPVNGPAKGSPPVSLYCIHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRV
LKRKGFMIEDAQLETVSALQKLILISLQAALQVMVLKLSFDKEDEKLSSEIYFTSKEIALLHIVGKKSEGNTKIQQNPYK
KESMAWAAWIIARLGAWSAYKSQSIPGYITFKNGLDRFYTQFELYELIS

Sequences:

>Translated_369_residues
MKKKTASPGIVGQKQCGTFLHPVLVVDASSHIPIGFSSVKQWNRSPAALSREERNYRYQPIEEKESYRWIESGMAASEQM
PRDAVKTIIGDREADIFELFSRIPTDNVHLLIRSVHERNCRLDDPDCSVHLNTLMEQAVLRAEYSFEVLPGSGRKKRVAC
MELRFERVTLCAPVNGPAKGSPPVSLYCIHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRV
LKRKGFMIEDAQLETVSALQKLILISLQAALQVMVLKLSFDKEDEKLSSEIYFTSKEIALLHIVGKKSEGNTKIQQNPYK
KESMAWAAWIIARLGAWSAYKSQSIPGYITFKNGLDRFYTQFELYELIS
>Mature_369_residues
MKKKTASPGIVGQKQCGTFLHPVLVVDASSHIPIGFSSVKQWNRSPAALSREERNYRYQPIEEKESYRWIESGMAASEQM
PRDAVKTIIGDREADIFELFSRIPTDNVHLLIRSVHERNCRLDDPDCSVHLNTLMEQAVLRAEYSFEVLPGSGRKKRVAC
MELRFERVTLCAPVNGPAKGSPPVSLYCIHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRV
LKRKGFMIEDAQLETVSALQKLILISLQAALQVMVLKLSFDKEDEKLSSEIYFTSKEIALLHIVGKKSEGNTKIQQNPYK
KESMAWAAWIIARLGAWSAYKSQSIPGYITFKNGLDRFYTQFELYELIS

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 42150; Mature: 42150

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKTASPGIVGQKQCGTFLHPVLVVDASSHIPIGFSSVKQWNRSPAALSREERNYRYQP
CCCCCCCCCCCCHHHCCHHHCEEEEEECCCCCCCCHHHHHHCCCCCHHHCHHHCCCCCCC
IEEKESYRWIESGMAASEQMPRDAVKTIIGDREADIFELFSRIPTDNVHLLIRSVHERNC
CCHHHHHHHHHHCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
RLDDPDCSVHLNTLMEQAVLRAEYSFEVLPGSGRKKRVACMELRFERVTLCAPVNGPAKG
CCCCCCCCEEHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCEEEEEECCCCCCCC
SPPVSLYCIHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRV
CCCEEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKRKGFMIEDAQLETVSALQKLILISLQAALQVMVLKLSFDKEDEKLSSEIYFTSKEIAL
HHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEECCCEEE
LHIVGKKSEGNTKIQQNPYKKESMAWAAWIIARLGAWSAYKSQSIPGYITFKNGLDRFYT
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCHHHHHH
QFELYELIS
HHHHHHHCC
>Mature Secondary Structure
MKKKTASPGIVGQKQCGTFLHPVLVVDASSHIPIGFSSVKQWNRSPAALSREERNYRYQP
CCCCCCCCCCCCHHHCCHHHCEEEEEECCCCCCCCHHHHHHCCCCCHHHCHHHCCCCCCC
IEEKESYRWIESGMAASEQMPRDAVKTIIGDREADIFELFSRIPTDNVHLLIRSVHERNC
CCHHHHHHHHHHCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
RLDDPDCSVHLNTLMEQAVLRAEYSFEVLPGSGRKKRVACMELRFERVTLCAPVNGPAKG
CCCCCCCCEEHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCEEEEEECCCCCCCC
SPPVSLYCIHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRV
CCCEEEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKRKGFMIEDAQLETVSALQKLILISLQAALQVMVLKLSFDKEDEKLSSEIYFTSKEIAL
HHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEECCCEEE
LHIVGKKSEGNTKIQQNPYKKESMAWAAWIIARLGAWSAYKSQSIPGYITFKNGLDRFYT
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCHHHHHH
QFELYELIS
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]