Click here to switch to the map view.

The map label for this gene is ksgA [H]

Identifier: 29349451

GI number: 29349451

Start: 5259406

End: 5260209

Strand: Reverse

Name: ksgA [H]

Synonym: BT_4043

Alternate gene names: 29349451

Gene position: 5260209-5259406 (Counterclockwise)

Preceding gene: 29349454

Following gene: 29349450

Centisome position: 84.02

GC content: 47.51

Gene sequence:

>804_bases
ATGAAATTAGTAAAGCCTAAAAAGTTTCTCGGACAGCACTTTCTGAAAGATCTGAAAGTAGCGCAGGATATTGCCGATAC
AGTCGACACCTTCCCCGAACTGCCTGTATTGGAGGTAGGACCGGGTATGGGAGTATTGACCCAGTTTCTGGTAAAAAAAG
ACCGGTTGGTAAAAGTCGTTGAAGTAGACTACGAATCGGTTGCCTATCTCCGTGAAGCCTATCCGTCACTGGAGGATCAC
ATTATCGAAGACGATTTTCTGAAAATGAACCTTCACCGGTTGTTCGACGGAAAGCCTTTCGTACTGACCGGTAATTACCC
GTACAACATCTCCAGCCAGATATTTTTCAAGATGCTGGAAAACAAGGACATCATCCCTTGCTGTACCGGAATGATACAGA
AAGAAGTAGCCGAACGTATCGCTGCCGGACCGGGCAGCAAAACCTACGGCATCCTGAGCGTACTGATACAGGCATGGTAT
AAAGTAGAATATCTCTTTACCGTCAGCGAACATGTTTTCAACCCGCCCCCAAAAGTGAAAAGCGCCGTCATCCGCATGAC
CCGCAATGATACCAAGGAACTGGGATGCGACGAAAAACTGTTCAAACAAGTGGTGAAGACCACCTTCAACCAACGACGCA
AAACATTACGCAACTCTATCAAGCCGATTTTAGGCAAAGACTGCCCGCTGACGGAGGATGCATTATTTAACAAGCGCCCG
GAACAACTGTCGGTGGAAGAATTCATCAGTCTGACCAACCAGGTGGAAGAAGCGCTGAAAACAGCCACCGCATCCGGTAA
CTGA

Upstream 100 bases:

>100_bases
CTTCAGCGTCTTTTTTAGTAGTTTCTTCATCCTTAATTCTTCATTCTTCATTTAAAAGAATTACCTTTGTCGGCTGCAAA
AGTAATTATTTAGTTGGTAA

Downstream 100 bases:

>100_bases
CCGATAGTGAGCGAAACCCGTAATTTGTAAAACGTTTAATCGTAAACAAAAAGATGAACGAGGAATACATTGACAACGTA
AAACACCTTATCGAGCAAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKLVKPKKFLGQHFLKDLKVAQDIADTVDTFPELPVLEVGPGMGVLTQFLVKKDRLVKVVEVDYESVAYLREAYPSLEDH
IIEDDFLKMNLHRLFDGKPFVLTGNYPYNISSQIFFKMLENKDIIPCCTGMIQKEVAERIAAGPGSKTYGILSVLIQAWY
KVEYLFTVSEHVFNPPPKVKSAVIRMTRNDTKELGCDEKLFKQVVKTTFNQRRKTLRNSIKPILGKDCPLTEDALFNKRP
EQLSVEEFISLTNQVEEALKTATASGN

Sequences:

>Translated_267_residues
MKLVKPKKFLGQHFLKDLKVAQDIADTVDTFPELPVLEVGPGMGVLTQFLVKKDRLVKVVEVDYESVAYLREAYPSLEDH
IIEDDFLKMNLHRLFDGKPFVLTGNYPYNISSQIFFKMLENKDIIPCCTGMIQKEVAERIAAGPGSKTYGILSVLIQAWY
KVEYLFTVSEHVFNPPPKVKSAVIRMTRNDTKELGCDEKLFKQVVKTTFNQRRKTLRNSIKPILGKDCPLTEDALFNKRP
EQLSVEEFISLTNQVEEALKTATASGN
>Mature_267_residues
MKLVKPKKFLGQHFLKDLKVAQDIADTVDTFPELPVLEVGPGMGVLTQFLVKKDRLVKVVEVDYESVAYLREAYPSLEDH
IIEDDFLKMNLHRLFDGKPFVLTGNYPYNISSQIFFKMLENKDIIPCCTGMIQKEVAERIAAGPGSKTYGILSVLIQAWY
KVEYLFTVSEHVFNPPPKVKSAVIRMTRNDTKELGCDEKLFKQVVKTTFNQRRKTLRNSIKPILGKDCPLTEDALFNKRP
EQLSVEEFISLTNQVEEALKTATASGN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=217, Percent_Identity=35.0230414746544, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI156415992, Length=279, Percent_Identity=30.1075268817204, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1786236, Length=256, Percent_Identity=32.8125, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25146882, Length=181, Percent_Identity=36.4640883977901, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25141369, Length=292, Percent_Identity=22.6027397260274, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324989, Length=217, Percent_Identity=33.1797235023041, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358017, Length=185, Percent_Identity=35.1351351351351, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21357273, Length=284, Percent_Identity=29.5774647887324, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 30364; Mature: 30364

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA