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The map label for this gene is recJ [H]

Identifier: 29349347

GI number: 29349347

Start: 5114434

End: 5116152

Strand: Reverse

Name: recJ [H]

Synonym: BT_3939

Alternate gene names: 29349347

Gene position: 5116152-5114434 (Counterclockwise)

Preceding gene: 29349350

Following gene: 29349346

Centisome position: 81.72

GC content: 48.34

Gene sequence:

>1719_bases
ATGAATCACAAATGGAATTATCAACCCATTACACCCGAACAGGCAGAGACAAGCCAGACATTGGCCCAGGAATTAGGCAT
TAGCCCGATACTTGGACAACTTTTGGTGCAGCGGGGAATAACGAAGGCAGGAGATGCCAAGAAGTTTTTCCGCCCGCAAT
TGCCCGACCTGCACGATCCGTTCCTGATGAAGGACATGGACATTGCAGTAGAACGTCTGAACAAGGCGATGGGAAAGAAA
GAGCGTATTCTGATTTATGGAGATTATGATGTAGACGGTACTACTGCCGTAGCATTGGTCTACAAGTTTATTCAACAGTT
CTATTCGAACATTGACTATTACATACCCGACCGCTACAACGAAGGGTATGGCATTTCTAAAAAAGGAGTTGACTATGCCT
CAGAGACTGGCGTAGGATTAATCATCGTACTCGACTGCGGAATCAAAGCAGTGGAGGAAATAACGTATGCGAAAGAAAAG
GGAATCGACTTTATCATCTGCGACCATCATGTGCCGGACGATATCCTCCCCCCTGCCGTCGCAATCCTGAATGCCAAACG
CCTGGACAACACTTATCCATACACCCACCTTTCCGGTTGCGGAGTTGGTTTTAAGTTTATGCAGGCATTTGCTATCAACA
ATGGTATTGAATTTCATCATCTGATTCCATTGCTCGACCTCGTAGCGGTGAGCATTGCATCGGACATCGTCCCGATCATG
GGCGAAAACCGTATTCTCGCCTATCACGGCCTGAAGCAACTGAACAGCAACCCCAGCGTCGGCATGAAAGCCATCATCGA
TGTATGCGGACTCTCCGAAAAGGAGATCACCGTCAGCGATATCGTGTTCAAAATCGGACCACGTATCAACGCATCGGGAC
GCATCCAGAACGGTAAGGAAGCGGTGGACCTCCTCACAGAGAAGGACTTTTCTGCCGCACTGGAGAAAGCGGGACAGATC
AATCAGTACAACGAAACCCGGAAAGACCTCGACAAGAGCATGACAGAAGAAGCTAACAATATTGTGGCCAATCTGGAAGG
GCTGTCCGAACGCCGTTCCATCGTGCTTTATAATGAGGAATGGCACAAAGGTGTCATCGGCATCGTAGCATCCCGTCTGA
CAGAAGTTTACTACCGCCCGGCAGTAGTACTCACACGGACGGATGATATGGCCACCGGTTCCGCACGTTCCGTCTCCGGA
TTTGATGTGTACAAAGCGATTGAACATTGCCGCGACCTGTTGGAGAACTTCGGCGGACATACCTATGCAGCGGGGTTATC
CATGAAAGTGGAGAATGTGGAAGCCTTTACCCGACGATTCGAAGAATATGTGTCACAACATATTCTGCCGGAACAAACCA
GCGCCGTCATCAATATAGATGCGGAGATCGACTTCAGAGACATCACATCGAAATTCTTCAATGACCTGAAGAAATTCAAC
CCTTTCGGTCCGGACAATATCAAACCGATCTTCTGTACGCATCACGTCTATGACTACGGAACCAGCAAAGTAGTGGGACG
CGATCAGGAACATATCAAGCTGGAACTGGTTGACAACAAGTCGAATAACGTCATGAACGGCATCGCATTCGGACAGAGTT
CTCATGTGCGCTATATCAAGACGAAGCGTTCATTCGACATCTGCTATACGATCGAGGAGAACACGCACAAGCGTGGCGAA
GTCCAACTTCAAATCGAGGATATAAAGCCGATTGAATAA

Upstream 100 bases:

>100_bases
TCTACTATACCCTTCCGCTTTTTTTCGTACTTTTACCCCTTGTTGGAAAGTAGCGCCTTTCTGACCCCTGAGAACAGAAA
AAGAAAAAACAATATATAAA

Downstream 100 bases:

>100_bases
GTATCAGGAGATATTAAAACAATACTGGGGTTATGACTCCTTCCGCGACTTGCAGGAGGAGATCATCACCAGTATCGGCG
AAGGCAAAGACACACTGGGA

Product: single-stranded-DNA-specific exonuclease recJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MNHKWNYQPITPEQAETSQTLAQELGISPILGQLLVQRGITKAGDAKKFFRPQLPDLHDPFLMKDMDIAVERLNKAMGKK
ERILIYGDYDVDGTTAVALVYKFIQQFYSNIDYYIPDRYNEGYGISKKGVDYASETGVGLIIVLDCGIKAVEEITYAKEK
GIDFIICDHHVPDDILPPAVAILNAKRLDNTYPYTHLSGCGVGFKFMQAFAINNGIEFHHLIPLLDLVAVSIASDIVPIM
GENRILAYHGLKQLNSNPSVGMKAIIDVCGLSEKEITVSDIVFKIGPRINASGRIQNGKEAVDLLTEKDFSAALEKAGQI
NQYNETRKDLDKSMTEEANNIVANLEGLSERRSIVLYNEEWHKGVIGIVASRLTEVYYRPAVVLTRTDDMATGSARSVSG
FDVYKAIEHCRDLLENFGGHTYAAGLSMKVENVEAFTRRFEEYVSQHILPEQTSAVINIDAEIDFRDITSKFFNDLKKFN
PFGPDNIKPIFCTHHVYDYGTSKVVGRDQEHIKLELVDNKSNNVMNGIAFGQSSHVRYIKTKRSFDICYTIEENTHKRGE
VQLQIEDIKPIE

Sequences:

>Translated_572_residues
MNHKWNYQPITPEQAETSQTLAQELGISPILGQLLVQRGITKAGDAKKFFRPQLPDLHDPFLMKDMDIAVERLNKAMGKK
ERILIYGDYDVDGTTAVALVYKFIQQFYSNIDYYIPDRYNEGYGISKKGVDYASETGVGLIIVLDCGIKAVEEITYAKEK
GIDFIICDHHVPDDILPPAVAILNAKRLDNTYPYTHLSGCGVGFKFMQAFAINNGIEFHHLIPLLDLVAVSIASDIVPIM
GENRILAYHGLKQLNSNPSVGMKAIIDVCGLSEKEITVSDIVFKIGPRINASGRIQNGKEAVDLLTEKDFSAALEKAGQI
NQYNETRKDLDKSMTEEANNIVANLEGLSERRSIVLYNEEWHKGVIGIVASRLTEVYYRPAVVLTRTDDMATGSARSVSG
FDVYKAIEHCRDLLENFGGHTYAAGLSMKVENVEAFTRRFEEYVSQHILPEQTSAVINIDAEIDFRDITSKFFNDLKKFN
PFGPDNIKPIFCTHHVYDYGTSKVVGRDQEHIKLELVDNKSNNVMNGIAFGQSSHVRYIKTKRSFDICYTIEENTHKRGE
VQLQIEDIKPIE
>Mature_572_residues
MNHKWNYQPITPEQAETSQTLAQELGISPILGQLLVQRGITKAGDAKKFFRPQLPDLHDPFLMKDMDIAVERLNKAMGKK
ERILIYGDYDVDGTTAVALVYKFIQQFYSNIDYYIPDRYNEGYGISKKGVDYASETGVGLIIVLDCGIKAVEEITYAKEK
GIDFIICDHHVPDDILPPAVAILNAKRLDNTYPYTHLSGCGVGFKFMQAFAINNGIEFHHLIPLLDLVAVSIASDIVPIM
GENRILAYHGLKQLNSNPSVGMKAIIDVCGLSEKEITVSDIVFKIGPRINASGRIQNGKEAVDLLTEKDFSAALEKAGQI
NQYNETRKDLDKSMTEEANNIVANLEGLSERRSIVLYNEEWHKGVIGIVASRLTEVYYRPAVVLTRTDDMATGSARSVSG
FDVYKAIEHCRDLLENFGGHTYAAGLSMKVENVEAFTRRFEEYVSQHILPEQTSAVINIDAEIDFRDITSKFFNDLKKFN
PFGPDNIKPIFCTHHVYDYGTSKVVGRDQEHIKLELVDNKSNNVMNGIAFGQSSHVRYIKTKRSFDICYTIEENTHKRGE
VQLQIEDIKPIE

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=584, Percent_Identity=31.1643835616438, Blast_Score=285, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 64306; Mature: 64306

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKWNYQPITPEQAETSQTLAQELGISPILGQLLVQRGITKAGDAKKFFRPQLPDLHDP
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCC
FLMKDMDIAVERLNKAMGKKERILIYGDYDVDGTTAVALVYKFIQQFYSNIDYYIPDRYN
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCC
EGYGISKKGVDYASETGVGLIIVLDCGIKAVEEITYAKEKGIDFIICDHHVPDDILPPAV
CCCCCCCCCCCCHHCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHH
AILNAKRLDNTYPYTHLSGCGVGFKFMQAFAINNGIEFHHLIPLLDLVAVSIASDIVPIM
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GENRILAYHGLKQLNSNPSVGMKAIIDVCGLSEKEITVSDIVFKIGPRINASGRIQNGKE
CCCEEEEECCHHHHCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCCCCCCCCHH
AVDLLTEKDFSAALEKAGQINQYNETRKDLDKSMTEEANNIVANLEGLSERRSIVLYNEE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
WHKGVIGIVASRLTEVYYRPAVVLTRTDDMATGSARSVSGFDVYKAIEHCRDLLENFGGH
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TYAAGLSMKVENVEAFTRRFEEYVSQHILPEQTSAVINIDAEIDFRDITSKFFNDLKKFN
EEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
PFGPDNIKPIFCTHHVYDYGTSKVVGRDQEHIKLELVDNKSNNVMNGIAFGQSSHVRYIK
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEHEECCCCCCEEEEE
TKRSFDICYTIEENTHKRGEVQLQIEDIKPIE
ECCCEEEEEEEECCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MNHKWNYQPITPEQAETSQTLAQELGISPILGQLLVQRGITKAGDAKKFFRPQLPDLHDP
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCC
FLMKDMDIAVERLNKAMGKKERILIYGDYDVDGTTAVALVYKFIQQFYSNIDYYIPDRYN
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCC
EGYGISKKGVDYASETGVGLIIVLDCGIKAVEEITYAKEKGIDFIICDHHVPDDILPPAV
CCCCCCCCCCCCHHCCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHH
AILNAKRLDNTYPYTHLSGCGVGFKFMQAFAINNGIEFHHLIPLLDLVAVSIASDIVPIM
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GENRILAYHGLKQLNSNPSVGMKAIIDVCGLSEKEITVSDIVFKIGPRINASGRIQNGKE
CCCEEEEECCHHHHCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCCCCCCCCHH
AVDLLTEKDFSAALEKAGQINQYNETRKDLDKSMTEEANNIVANLEGLSERRSIVLYNEE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
WHKGVIGIVASRLTEVYYRPAVVLTRTDDMATGSARSVSGFDVYKAIEHCRDLLENFGGH
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
TYAAGLSMKVENVEAFTRRFEEYVSQHILPEQTSAVINIDAEIDFRDITSKFFNDLKKFN
EEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
PFGPDNIKPIFCTHHVYDYGTSKVVGRDQEHIKLELVDNKSNNVMNGIAFGQSSHVRYIK
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEHEECCCCCCEEEEE
TKRSFDICYTIEENTHKRGEVQLQIEDIKPIE
ECCCEEEEEEEECCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]