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The map label for this gene is pycB [H]

Identifier: 29346606

GI number: 29346606

Start: 1492071

End: 1493846

Strand: Reverse

Name: pycB [H]

Synonym: BT_1196

Alternate gene names: 29346606

Gene position: 1493846-1492071 (Counterclockwise)

Preceding gene: 29346607

Following gene: 29346602

Centisome position: 23.86

GC content: 48.42

Gene sequence:

>1776_bases
ATGAAAAAGGAAATCAAATTCAGTCTCGTTTATCGGGATATGTGGCAGTCGTCCGGCAAGTATCAACCGCGTGCCGATCA
GTTAGTAAGAATTGCTCCGTTAATTATCGAAATGGGTTGTTTCTCCCGTGTAGAAACCAACGGAGGCGCGTTCGAACAAG
TCAACCTACTGTATGGCGAGAACCCGAACAAAGCCGTCCGGGCTTTCACGAAACCTTTCCACGAAGCCAGCATACAAACT
CATATGCTCGACCGTGGTCTGAATGGTCTTCGAATGTATCCCGTTCCGGCAGATGTGCGTCGGTTAATGTATAAAGTGAA
ACATGCGCAGGGAGTGGATATCACCCGTATCTTTTGCGGCTTGAATGAAGTCAGAAATATTATTCCTTCCATTCATTATG
CCCTCGAAGCGGGGATGATTCCGCAGGCAACGTTATGTATTACGTTCTCGCCCGTGCATACCGTAGAATATTATACCGCC
ATCGCCGAGCGTCTGATAGAAGCTGGCGCACCGGAAATCTGTCTGAAAGATATGGCAGGTGTCGGTCGCCCCGAAATGCT
GGGACGTCTGACGAAAGCAATCAAGGAACGGCATCCCGAAATCATCATCCAATATCACGGACATAGCGGACCGGGGCTTT
CGATGGCTTCTATCCTCGAAGTTTGTGAGAATGGCGCGGATATTATTGATGTCGCAATGGAGCCGATTTCGTGGGGGAAA
GTTCATCCGGACGTTATCTCTGTGCAGGCTATGCTGAAAGACGCGGGATTTCAGGTACCGGAGATTAATATGAAAGCCTA
CATGAAAGCCCGTGCCATGACACAGGAGTTCATTGACGATTTTCTGGGTTATTTTATGGACCCTACCAACAAGCATATGT
CTTCTTTGCTCTTGAAATGCGGACTTCCCGGAGGTATGATGGGTTCTATGATGGCCGATCTGAAAGGAGTACATTCCGGC
ATCAATCTGATATTGAGAGGTAAGAACGAACCGGAACTGAGTATCGACGATTTGCTCGTGATGCTGTTTGACGAGGTGGA
ATATGTGTGGCCGAAACTCGGCTATCCTCCATTGGTGACTCCGTTCAGCCAATATGTGAAGAATGTGGCATTGATGAATG
TGATGTCTCTGATCAAGGGAGAGGAGCGTTGGACGATGATCGACAACCATACCTGGGATATGATTCTGGGTAAGAGTGGT
CGTTTGCCCGGTGCACTTGCTCCGGAGATTATCGCACTGGCAAAAGAAAAAGGATACGAATTTACGGACGAAGACCCGCA
GAAGAACTATCCGGATCAGCTGGACGAGTATCGTAAGGAAATGACAGAAAACGGCTGGGACTTCGGACAGGATGATGAAG
AACTCTTCGAACTGGCAATGCACGACCGCCAATATCGGGATTATAAATCGGGTATTGCCAAGAAACGTTTCGAAGATGAT
TTGCAGCGTGCCAAAGATGCCGCATTGGCAAAACAGGGATTCTCCGAAGAAGAAGTGAAACGGATGAAACGTGCCAAGGC
CGAACCGGTGACGGCAATGGAGAAAGGTCAGATCATTTGGGAGATTGATGTGGAATCGCCTTCTATGCCGCCCGAAGTGG
GACATAAATATGACCCGGACGATGTGTTCTGCTACATTGCTACTCCTTGGCATACATATGATAAGGTGTTGGCGAATTTT
AGCGGTCGTGTGATCGAAGTATGTGCCAAGCAGGGAGCATTGGTAGATAAAGGCGAACCATTGGCGTATATCGAGAGGTG
TGAGGAACCGGCATAG

Upstream 100 bases:

>100_bases
CCCAACTTATTCTCTCTATATTTGTTATTATGTCAGAAAACTCAATATATTTGTATCAACTAGTTAACCTAAGTAATATA
AATCTCATCTATTTACGGCA

Downstream 100 bases:

>100_bases
TCTGTATCTTAAATAACGGAAAAAAGAAATCCCTTTATCCTTGTCTTGTTTCTTGATGAAATAGACTGTGATAAAGGGAT
TGCTTTATTTCTTTTTCTTC

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MKKEIKFSLVYRDMWQSSGKYQPRADQLVRIAPLIIEMGCFSRVETNGGAFEQVNLLYGENPNKAVRAFTKPFHEASIQT
HMLDRGLNGLRMYPVPADVRRLMYKVKHAQGVDITRIFCGLNEVRNIIPSIHYALEAGMIPQATLCITFSPVHTVEYYTA
IAERLIEAGAPEICLKDMAGVGRPEMLGRLTKAIKERHPEIIIQYHGHSGPGLSMASILEVCENGADIIDVAMEPISWGK
VHPDVISVQAMLKDAGFQVPEINMKAYMKARAMTQEFIDDFLGYFMDPTNKHMSSLLLKCGLPGGMMGSMMADLKGVHSG
INLILRGKNEPELSIDDLLVMLFDEVEYVWPKLGYPPLVTPFSQYVKNVALMNVMSLIKGEERWTMIDNHTWDMILGKSG
RLPGALAPEIIALAKEKGYEFTDEDPQKNYPDQLDEYRKEMTENGWDFGQDDEELFELAMHDRQYRDYKSGIAKKRFEDD
LQRAKDAALAKQGFSEEEVKRMKRAKAEPVTAMEKGQIIWEIDVESPSMPPEVGHKYDPDDVFCYIATPWHTYDKVLANF
SGRVIEVCAKQGALVDKGEPLAYIERCEEPA

Sequences:

>Translated_591_residues
MKKEIKFSLVYRDMWQSSGKYQPRADQLVRIAPLIIEMGCFSRVETNGGAFEQVNLLYGENPNKAVRAFTKPFHEASIQT
HMLDRGLNGLRMYPVPADVRRLMYKVKHAQGVDITRIFCGLNEVRNIIPSIHYALEAGMIPQATLCITFSPVHTVEYYTA
IAERLIEAGAPEICLKDMAGVGRPEMLGRLTKAIKERHPEIIIQYHGHSGPGLSMASILEVCENGADIIDVAMEPISWGK
VHPDVISVQAMLKDAGFQVPEINMKAYMKARAMTQEFIDDFLGYFMDPTNKHMSSLLLKCGLPGGMMGSMMADLKGVHSG
INLILRGKNEPELSIDDLLVMLFDEVEYVWPKLGYPPLVTPFSQYVKNVALMNVMSLIKGEERWTMIDNHTWDMILGKSG
RLPGALAPEIIALAKEKGYEFTDEDPQKNYPDQLDEYRKEMTENGWDFGQDDEELFELAMHDRQYRDYKSGIAKKRFEDD
LQRAKDAALAKQGFSEEEVKRMKRAKAEPVTAMEKGQIIWEIDVESPSMPPEVGHKYDPDDVFCYIATPWHTYDKVLANF
SGRVIEVCAKQGALVDKGEPLAYIERCEEPA
>Mature_591_residues
MKKEIKFSLVYRDMWQSSGKYQPRADQLVRIAPLIIEMGCFSRVETNGGAFEQVNLLYGENPNKAVRAFTKPFHEASIQT
HMLDRGLNGLRMYPVPADVRRLMYKVKHAQGVDITRIFCGLNEVRNIIPSIHYALEAGMIPQATLCITFSPVHTVEYYTA
IAERLIEAGAPEICLKDMAGVGRPEMLGRLTKAIKERHPEIIIQYHGHSGPGLSMASILEVCENGADIIDVAMEPISWGK
VHPDVISVQAMLKDAGFQVPEINMKAYMKARAMTQEFIDDFLGYFMDPTNKHMSSLLLKCGLPGGMMGSMMADLKGVHSG
INLILRGKNEPELSIDDLLVMLFDEVEYVWPKLGYPPLVTPFSQYVKNVALMNVMSLIKGEERWTMIDNHTWDMILGKSG
RLPGALAPEIIALAKEKGYEFTDEDPQKNYPDQLDEYRKEMTENGWDFGQDDEELFELAMHDRQYRDYKSGIAKKRFEDD
LQRAKDAALAKQGFSEEEVKRMKRAKAEPVTAMEKGQIIWEIDVESPSMPPEVGHKYDPDDVFCYIATPWHTYDKVLANF
SGRVIEVCAKQGALVDKGEPLAYIERCEEPA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=252, Percent_Identity=26.5873015873016, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI106049295, Length=252, Percent_Identity=26.5873015873016, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI106049292, Length=252, Percent_Identity=26.5873015873016, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17562816, Length=465, Percent_Identity=24.0860215053763, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319695, Length=333, Percent_Identity=25.5255255255255, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321376, Length=608, Percent_Identity=22.8618421052632, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652212, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652210, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652214, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19921944, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652216, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281363050, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652224, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652222, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652220, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652218, Length=252, Percent_Identity=31.7460317460317, Blast_Score=111, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 66835; Mature: 66835

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEIKFSLVYRDMWQSSGKYQPRADQLVRIAPLIIEMGCFSRVETNGGAFEQVNLLYGE
CCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
NPNKAVRAFTKPFHEASIQTHMLDRGLNGLRMYPVPADVRRLMYKVKHAQGVDITRIFCG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHCCCCCHHHHHHH
LNEVRNIIPSIHYALEAGMIPQATLCITFSPVHTVEYYTAIAERLIEAGAPEICLKDMAG
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
VGRPEMLGRLTKAIKERHPEIIIQYHGHSGPGLSMASILEVCENGADIIDVAMEPISWGK
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEHHHHCCCCCCC
VHPDVISVQAMLKDAGFQVPEINMKAYMKARAMTQEFIDDFLGYFMDPTNKHMSSLLLKC
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GLPGGMMGSMMADLKGVHSGINLILRGKNEPELSIDDLLVMLFDEVEYVWPKLGYPPLVT
CCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PFSQYVKNVALMNVMSLIKGEERWTMIDNHTWDMILGKSGRLPGALAPEIIALAKEKGYE
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCC
FTDEDPQKNYPDQLDEYRKEMTENGWDFGQDDEELFELAMHDRQYRDYKSGIAKKRFEDD
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRAKDAALAKQGFSEEEVKRMKRAKAEPVTAMEKGQIIWEIDVESPSMPPEVGHKYDPD
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHCCCEEEEEECCCCCCCHHCCCCCCCC
DVFCYIATPWHTYDKVLANFSGRVIEVCAKQGALVDKGEPLAYIERCEEPA
CEEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MKKEIKFSLVYRDMWQSSGKYQPRADQLVRIAPLIIEMGCFSRVETNGGAFEQVNLLYGE
CCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
NPNKAVRAFTKPFHEASIQTHMLDRGLNGLRMYPVPADVRRLMYKVKHAQGVDITRIFCG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHCCCCCHHHHHHH
LNEVRNIIPSIHYALEAGMIPQATLCITFSPVHTVEYYTAIAERLIEAGAPEICLKDMAG
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
VGRPEMLGRLTKAIKERHPEIIIQYHGHSGPGLSMASILEVCENGADIIDVAMEPISWGK
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEHHHHCCCCCCC
VHPDVISVQAMLKDAGFQVPEINMKAYMKARAMTQEFIDDFLGYFMDPTNKHMSSLLLKC
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GLPGGMMGSMMADLKGVHSGINLILRGKNEPELSIDDLLVMLFDEVEYVWPKLGYPPLVT
CCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PFSQYVKNVALMNVMSLIKGEERWTMIDNHTWDMILGKSGRLPGALAPEIIALAKEKGYE
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCC
FTDEDPQKNYPDQLDEYRKEMTENGWDFGQDDEELFELAMHDRQYRDYKSGIAKKRFEDD
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRAKDAALAKQGFSEEEVKRMKRAKAEPVTAMEKGQIIWEIDVESPSMPPEVGHKYDPD
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHCCCEEEEEECCCCCCCHHCCCCCCCC
DVFCYIATPWHTYDKVLANFSGRVIEVCAKQGALVDKGEPLAYIERCEEPA
CEEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]