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The map label for this gene is pfkA

Identifier: 29346512

GI number: 29346512

Start: 1391513

End: 1392496

Strand: Reverse

Name: pfkA

Synonym: BT_1102

Alternate gene names: 29346512

Gene position: 1392496-1391513 (Counterclockwise)

Preceding gene: 29346513

Following gene: 29346511

Centisome position: 22.24

GC content: 49.19

Gene sequence:

>984_bases
ATGGATAACAAGTATATTGGAATTCTGACTTCCGGAGGGGATGCTTCAGGAATGAACGCGGCTATCCGTGCCGTGACCCG
CGCCGCCATTTTTAACGGATTTAAAGTGAAAGGAATTTATAGAGGTTACGAGGGGCTGATTGCCGGTGAAGTGAAAGAAC
TCACGACGGAAGATGTAAGTAGCATTATCCAGAGGGGAGGCACCATACTGAAAACAGCTCGCTCCGAGACTTTCACCACT
CCCGAAGGACGCAAGAAAGCTTATAAGGTCATACAGAAAGAGAACATCAATGCTTTGATAATTATCGGTGGAGACGGTTC
CCTGACGGGAGCCCGTATCTTTGCGGAGGAGTATGATGTGACGTGCATAGGATTGCCCGGCACCATTGACAATGACCTAT
ATGGTACCGATTTTACCATAGGATATGATACGGCATTGAACACCATTGTGGAATGCGTGGATAAAATCAGGGACACGGCA
ACCTCCCATGACCGTATCTTCTTTGTCGAAGTGATGGGGCGTGATGCGGGCTTCCTGGCACAAAACAGCGCAATAGCTTC
CGGTGCTGAAGCGGCCATCATCCCGGAGGACAGGACGGACGTGGACCAGTTGGAAACATTCATCGGGCGCGGATTCAGAA
AGACGAAAAACAGCAGCATCGTAATTGTGACCGAAAGTCCTGAAAACAAGAATGGAGGTGCCATATATTACGCCGACCGG
GTAAAGAAGGAATATCCCGGGTATGATGTCAGGGTTTCTATCCTGGGCCATCTGCAACGGGGTGGTGCTCCGAGTGCCAA
TGACCGTATACTGGCAAGCCGGCTGGGTGAAGCCGCAATCCAGGCCTTAATGGAAGGCCAGCGAAATGTTATGATAGGCA
TACGGAACAACGAGATTGTTTATGTTCCTTTTGTCCAGGCAATCAAAAAGGACAAGCCTATTGACAAAAGCCTGATCCGG
GTCCTTAACGAATTATCGATCTAA

Upstream 100 bases:

>100_bases
TCACTGAGTACGATAAAAAGCCGTATCTTTTATAGCAGAAAAAGGCTAAAACAGTTGCTTTCTAATTTTGCCTGATTAAT
TAAAATATAAAAAACAAATT

Downstream 100 bases:

>100_bases
AATAACCGTTAATATGTCAGAAGTTGCTCCTTTAATATCGGATCTTGCCATAATTCTGATTATTGCCGGTATTGTAACCG
TCATTTTCAAGTGGCTGAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 1; Phosphohexokinase 1

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVSSIIQRGGTILKTARSETFTT
PEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDVTCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTA
TSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
VKKEYPGYDVRVSILGHLQRGGAPSANDRILASRLGEAAIQALMEGQRNVMIGIRNNEIVYVPFVQAIKKDKPIDKSLIR
VLNELSI

Sequences:

>Translated_327_residues
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVSSIIQRGGTILKTARSETFTT
PEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDVTCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTA
TSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
VKKEYPGYDVRVSILGHLQRGGAPSANDRILASRLGEAAIQALMEGQRNVMIGIRNNEIVYVPFVQAIKKDKPIDKSLIR
VLNELSI
>Mature_327_residues
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVSSIIQRGGTILKTARSETFTT
PEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDVTCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTA
TSHDRIFFVEVMGRDAGFLAQNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
VKKEYPGYDVRVSILGHLQRGGAPSANDRILASRLGEAAIQALMEGQRNVMIGIRNNEIVYVPFVQAIKKDKPIDKSLIR
VLNELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI48762920, Length=349, Percent_Identity=43.2664756446991, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI266453619, Length=348, Percent_Identity=41.6666666666667, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI266453768, Length=348, Percent_Identity=41.6666666666667, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI266453748, Length=348, Percent_Identity=41.6666666666667, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI4505749, Length=348, Percent_Identity=41.6666666666667, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI11321601, Length=348, Percent_Identity=41.3793103448276, Blast_Score=246, Evalue=1e-65,
Organism=Escherichia coli, GI1790350, Length=309, Percent_Identity=46.2783171521036, Blast_Score=247, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI25147584, Length=352, Percent_Identity=43.1818181818182, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=38.7096774193548, Blast_Score=200, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=339, Percent_Identity=30.0884955752212, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6321679, Length=344, Percent_Identity=39.8255813953488, Blast_Score=219, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6323861, Length=350, Percent_Identity=36, Blast_Score=200, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28573326, Length=348, Percent_Identity=40.5172413793103, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24652337, Length=348, Percent_Identity=40.2298850574713, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI17647809, Length=348, Percent_Identity=40.2298850574713, Blast_Score=237, Evalue=8e-63,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF1_BACTN (Q8A8R5)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_810015.1
- ProteinModelPortal:   Q8A8R5
- SMR:   Q8A8R5
- GeneID:   1073299
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_1102
- NMPDR:   fig|226186.1.peg.1102
- HOGENOM:   HBG405044
- OMA:   QKENINA
- PhylomeDB:   Q8A8R5
- ProtClustDB:   PRK03202
- BioCyc:   BTHE226186:BT_1102-MONOMER
- BRENDA:   2.7.1.11
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 35512; Mature: 35512

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 130-130 BINDING 165-165 BINDING 251-251 BINDING 257-257 BINDING 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
SIIQRGGTILKTARSETFTTPEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDV
HHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCE
TCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTATSHDRIFFVEVMGRDAGFLA
EEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEE
QNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEHH
VKKEYPGYDVRVSILGHLQRGGAPSANDRILASRLGEAAIQALMEGQRNVMIGIRNNEIV
HHHHCCCCEEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEE
YVPFVQAIKKDKPIDKSLIRVLNELSI
EEEHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDNKYIGILTSGGDASGMNAAIRAVTRAAIFNGFKVKGIYRGYEGLIAGEVKELTTEDVS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
SIIQRGGTILKTARSETFTTPEGRKKAYKVIQKENINALIIIGGDGSLTGARIFAEEYDV
HHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCE
TCIGLPGTIDNDLYGTDFTIGYDTALNTIVECVDKIRDTATSHDRIFFVEVMGRDAGFLA
EEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEE
QNSAIASGAEAAIIPEDRTDVDQLETFIGRGFRKTKNSSIVIVTESPENKNGGAIYYADR
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEEHH
VKKEYPGYDVRVSILGHLQRGGAPSANDRILASRLGEAAIQALMEGQRNVMIGIRNNEIV
HHHHCCCCEEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEE
YVPFVQAIKKDKPIDKSLIRVLNELSI
EEEHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928