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The map label for this gene is arcB [H]

Identifier: 29144744

GI number: 29144744

Start: 4646234

End: 4647238

Strand: Reverse

Name: arcB [H]

Synonym: t4499

Alternate gene names: 29144744

Gene position: 4647238-4646234 (Counterclockwise)

Preceding gene: 29144745

Following gene: 29144743

Centisome position: 96.98

GC content: 55.72

Gene sequence:

>1005_bases
ATGGTTATTTCACTGAAAAATCGTAACTTTTTGAAGCTGCTTGACTACACCCCGGCGGAAATCCAACACCTCATCGATCT
GGCTATTGAGCTGAAAGCCGCAAAAAAAGCCGGATGCGAAAAGCAAACACTGATCGGCAAAAACATCGCGCTGATTTTTG
AAAAAACCTCTACTCGCACCCGATGCGCGTTTGAAGTCGCCGCGTTCGACCAGGGCGCGCAGGTGACTTATCTCGGCCCA
AGCGGCTCGCAGATCGGCCATAAAGAGTCCATGAAAGATACTGCTCGCGTCCTGGGGCGGATGTATGACGGCATCGAATA
TCGCGGATTTGGTCAACACATTGTGGAAGAGCTGGGGGAATACGCGGGCGTTCCCGTCTGGAACGGCCTGACCGATGAAT
TTCACCCCACACAGATCCTGGCCGATCTGATGACCATGCTGGAACATGCGCCGGGCAAAACGCTGCCGGAACTGAGCTTC
GCCTACCTGGGCGACGCGCGCAACAACATGGGCAATTCGCTGATGGTGGGCGCGGCGAAGATGGGCATGGATATCCGTCT
GATAGCGCCAAAGTCCTTCTGGCCTGATGCCGCGCTGGTCGCGCAGTGCCGGGAAATCGCCAGCGTTACCGGCGCTCGTA
TCACCTTAACGGAGAGCGTTGAAGATGGCGTTCACGGTGTGGATTTCCTCTATACCGACGTCTGGGTCTCGATGGGAGAA
CCCAAAGAAGCCTGGGCGGAGCGCGTCAGTCTGATGACGCCATATCAGGTCAACCAGCAGGTGGTAAACGCTACCGGTAA
TCCTGATGTGAAGTTTATGCACTGCCTGCCCGCCTTCCATAACGAGCACACCAAAGTGGGACGCGAAATCGAAATGGCTT
ACGGCCTGAAAGGACTGGAGGTCACGGAAGAGGTGTTCGAGTCCGCAGGCTCGATGGTCTTCGATGAAGCGGAAAACCGG
ATGCACACCATCAAAGCGGTCATGGTGGCGACGCTCGGCGACTAA

Upstream 100 bases:

>100_bases
GGATGGCGGCTTCCGCCTTATCCGGCCTACAGAGCGCGCATAACCGTGGGCCGGATAAGCGCAGCGCCATCAGGCAATTC
ATTTTAACAAGGATATTGTA

Downstream 100 bases:

>100_bases
TCCCACAGGATATGCGCCCTCGCGGCGCATATCCGTGATATCAGGAGAGAACATCATGGGTAAATTTAAATTTCCCACCG
CTTATACCATCCTGTTTATT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MVISLKNRNFLKLLDYTPAEIQHLIDLAIELKAAKKAGCEKQTLIGKNIALIFEKTSTRTRCAFEVAAFDQGAQVTYLGP
SGSQIGHKESMKDTARVLGRMYDGIEYRGFGQHIVEELGEYAGVPVWNGLTDEFHPTQILADLMTMLEHAPGKTLPELSF
AYLGDARNNMGNSLMVGAAKMGMDIRLIAPKSFWPDAALVAQCREIASVTGARITLTESVEDGVHGVDFLYTDVWVSMGE
PKEAWAERVSLMTPYQVNQQVVNATGNPDVKFMHCLPAFHNEHTKVGREIEMAYGLKGLEVTEEVFESAGSMVFDEAENR
MHTIKAVMVATLGD

Sequences:

>Translated_334_residues
MVISLKNRNFLKLLDYTPAEIQHLIDLAIELKAAKKAGCEKQTLIGKNIALIFEKTSTRTRCAFEVAAFDQGAQVTYLGP
SGSQIGHKESMKDTARVLGRMYDGIEYRGFGQHIVEELGEYAGVPVWNGLTDEFHPTQILADLMTMLEHAPGKTLPELSF
AYLGDARNNMGNSLMVGAAKMGMDIRLIAPKSFWPDAALVAQCREIASVTGARITLTESVEDGVHGVDFLYTDVWVSMGE
PKEAWAERVSLMTPYQVNQQVVNATGNPDVKFMHCLPAFHNEHTKVGREIEMAYGLKGLEVTEEVFESAGSMVFDEAENR
MHTIKAVMVATLGD
>Mature_334_residues
MVISLKNRNFLKLLDYTPAEIQHLIDLAIELKAAKKAGCEKQTLIGKNIALIFEKTSTRTRCAFEVAAFDQGAQVTYLGP
SGSQIGHKESMKDTARVLGRMYDGIEYRGFGQHIVEELGEYAGVPVWNGLTDEFHPTQILADLMTMLEHAPGKTLPELSF
AYLGDARNNMGNSLMVGAAKMGMDIRLIAPKSFWPDAALVAQCREIASVTGARITLTESVEDGVHGVDFLYTDVWVSMGE
PKEAWAERVSLMTPYQVNQQVVNATGNPDVKFMHCLPAFHNEHTKVGREIEMAYGLKGLEVTEEVFESAGSMVFDEAENR
MHTIKAVMVATLGD

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=333, Percent_Identity=37.2372372372372, Blast_Score=201, Evalue=5e-52,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=68.1957186544342, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1786469, Length=331, Percent_Identity=67.3716012084592, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI48994908, Length=378, Percent_Identity=24.8677248677249, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI2367364, Length=297, Percent_Identity=28.6195286195286, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322373, Length=329, Percent_Identity=32.5227963525836, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322331, Length=293, Percent_Identity=25.2559726962457, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 36835; Mature: 36835

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVISLKNRNFLKLLDYTPAEIQHLIDLAIELKAAKKAGCEKQTLIGKNIALIFEKTSTRT
CEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCC
RCAFEVAAFDQGAQVTYLGPSGSQIGHKESMKDTARVLGRMYDGIEYRGFGQHIVEELGE
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
YAGVPVWNGLTDEFHPTQILADLMTMLEHAPGKTLPELSFAYLGDARNNMGNSLMVGAAK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHCCCCCEEEEEHHH
MGMDIRLIAPKSFWPDAALVAQCREIASVTGARITLTESVEDGVHGVDFLYTDVWVSMGE
HCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHCCCCHHHHHHHHHHHCCCC
PKEAWAERVSLMTPYQVNQQVVNATGNPDVKFMHCLPAFHNEHTKVGREIEMAYGLKGLE
CHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEHHHHCCCHHHHCCEEHHHHCCCCHH
VTEEVFESAGSMVFDEAENRMHTIKAVMVATLGD
HHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVISLKNRNFLKLLDYTPAEIQHLIDLAIELKAAKKAGCEKQTLIGKNIALIFEKTSTRT
CEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCC
RCAFEVAAFDQGAQVTYLGPSGSQIGHKESMKDTARVLGRMYDGIEYRGFGQHIVEELGE
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
YAGVPVWNGLTDEFHPTQILADLMTMLEHAPGKTLPELSFAYLGDARNNMGNSLMVGAAK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHCCCCCEEEEEHHH
MGMDIRLIAPKSFWPDAALVAQCREIASVTGARITLTESVEDGVHGVDFLYTDVWVSMGE
HCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHCCCCHHHHHHHHHHHCCCC
PKEAWAERVSLMTPYQVNQQVVNATGNPDVKFMHCLPAFHNEHTKVGREIEMAYGLKGLE
CHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEHHHHCCCHHHHCCEEHHHHCCCCHH
VTEEVFESAGSMVFDEAENRMHTIKAVMVATLGD
HHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]