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The map label for this gene is yjfR [H]

Identifier: 29144678

GI number: 29144678

Start: 4582252

End: 4583316

Strand: Reverse

Name: yjfR [H]

Synonym: t4433

Alternate gene names: 29144678

Gene position: 4583316-4582252 (Counterclockwise)

Preceding gene: 29144685

Following gene: 29144677

Centisome position: 95.65

GC content: 52.02

Gene sequence:

>1065_bases
ATGAGTAAAGTACAAAGCATCACGCGTGAATCATGGATTCTGAGCACGTTTCCGGAGTGGGGAAGCTGGCTGAACGAAGA
GATTGAACAAGAGCAGGTCGCGCCGGGTACGTTCGCTATGTGGTGGCTGGGCTGTACCGGGATCTGGCTAAAATCTGAAG
GCGGCACTAACGTATGCGTCGATTTCTGGTGCGGTACCGGTAAACAGAGCCACGGTAATCCGTTGATGAAAACCGGTCAC
CAGATGCAGCGTATGGCTGGCGTCAAAAAATTACAGCCTAACCTGCGCACCACGCCGTTTGTTCTCGATCCTTTCGCTAT
CCGTCAGATCGACGCCGTCCTGGCGACCCACGACCATAACGATCACATTGACGTCAATGTCGCGGCTGCCGTAATGCAGA
ATTGCGCCGATGATGTTCCGTTTATCGGCCCGCAGACCTGCGTGGATTTATGGGTTGGCTGGGGCGTGCCGAAAGAACGC
TGCATCGTGGTAAAACCGGGCGATGTCGTAAAAGTGAAAGATATTGAAATTCACGCGCTTGATGCCTTTGACCGCACCGC
ATTAATCACCCTGCCCGCCGATCAGAAAGCCGCTGGCGTACTGCCGGACGGCATGGATGTACGCGCGGTTAACTATCTGT
TTAAAACGCCGGGCGGCAATCTCTATCACAGCGGCGATTCTCACTATTCCAACTATTACGCGAAACACGGCAACGAACAT
CAAATTGATGTCGCGCTTGGTTCCTATGGCGAAAACCCGCGCGGTATTACGGACAAAATGACCAGCGCGGATATTCTACG
CATGGCGGAGTCGCTGAACACCAAAGTGGTGATCCCGTTCCATCACGATATCTGGTCGAACTTCCAGGCCGATCCACAGG
AGATCCGCGTGTTATGGGAAATGAAGAAAGATCGCCTGAAGTATGGTTTTAAACCGTTTATCTGGCAGGTTGGCGGCAAG
TTTACCTGGCCGTTGGATAAAGACAATTTCGAATACCACTATCCGCGTGGTTTTGACGATTGCTTTACCATTGAACCCGA
TCTACCATTCAAATCCTTCCTGTGA

Upstream 100 bases:

>100_bases
GTCAAAAAATAGCCCATTGCCTTCTTGAGAAAATAGTGTCAGGATTAATCAAAACAAATCACGTTTTGAACCATTTTGAA
CAAACATGAAGGTAAATGCG

Downstream 100 bases:

>100_bases
GTCGATATCATGCCGGGATTACCCGGCATTTTTTCCATTTACTTTAGTATTTCCAGGCTATTTCAAATATCATCTTTTCA
AATCAATTTGTATCGGAATA

Product: L-ascorbate 6-phosphate lactonase

Products: NA

Alternate protein names: L-ascorbate utilization protein G [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSKVQSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKTGH
QMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPQTCVDLWVGWGVPKER
CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDVRAVNYLFKTPGGNLYHSGDSHYSNYYAKHGNEH
QIDVALGSYGENPRGITDKMTSADILRMAESLNTKVVIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGK
FTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL

Sequences:

>Translated_354_residues
MSKVQSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKTGH
QMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPQTCVDLWVGWGVPKER
CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDVRAVNYLFKTPGGNLYHSGDSHYSNYYAKHGNEH
QIDVALGSYGENPRGITDKMTSADILRMAESLNTKVVIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGK
FTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
>Mature_353_residues
SKVQSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKTGHQ
MQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPQTCVDLWVGWGVPKERC
IVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDVRAVNYLFKTPGGNLYHSGDSHYSNYYAKHGNEHQ
IDVALGSYGENPRGITDKMTSADILRMAESLNTKVVIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKF
TWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL

Specific function: Probably catalyzes the hydrolysis of L-ascorbate-6-P into 3-keto-L-gulonate-6-P. Is essential for L-ascorbate utilization under anaerobic conditions [H]

COG id: COG2220

COG function: function code R; Predicted Zn-dependent hydrolases of the beta-lactamase fold

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ulaG family [H]

Homologues:

Organism=Escherichia coli, GI87082387, Length=354, Percent_Identity=97.1751412429379, Blast_Score=724, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40073; Mature: 39942

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVQSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCV
CCHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCEEEE
DFWCGTGKQSHGNPLMKTGHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHN
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHEECCCC
DHIDVNVAAAVMQNCADDVPFIGPQTCVDLWVGWGVPKERCIVVKPGDVVKVKDIEIHAL
CCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEEEEEE
DAFDRTALITLPADQKAAGVLPDGMDVRAVNYLFKTPGGNLYHSGDSHYSNYYAKHGNEH
CCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCCE
QIDVALGSYGENPRGITDKMTSADILRMAESLNTKVVIPFHHDIWSNFQADPQEIRVLWE
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHH
MKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
HHHHHHHCCCCCEEEECCCEEECCCCCCCCCEECCCCCCCCEEECCCCCHHHCC
>Mature Secondary Structure 
SKVQSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCV
CHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCEEEE
DFWCGTGKQSHGNPLMKTGHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHN
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHEECCCC
DHIDVNVAAAVMQNCADDVPFIGPQTCVDLWVGWGVPKERCIVVKPGDVVKVKDIEIHAL
CCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEEEEEE
DAFDRTALITLPADQKAAGVLPDGMDVRAVNYLFKTPGGNLYHSGDSHYSNYYAKHGNEH
CCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCCE
QIDVALGSYGENPRGITDKMTSADILRMAESLNTKVVIPFHHDIWSNFQADPQEIRVLWE
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHH
MKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
HHHHHHHCCCCCEEEECCCEEECCCCCCCCCEECCCCCCCCEEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA