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The map label for this gene is yjeA [H]

Identifier: 29144645

GI number: 29144645

Start: 4545490

End: 4546467

Strand: Direct

Name: yjeA [H]

Synonym: t4396

Alternate gene names: 29144645

Gene position: 4545490-4546467 (Clockwise)

Preceding gene: 29144639

Following gene: 29144646

Centisome position: 94.86

GC content: 54.19

Gene sequence:

>978_bases
ATGAGCGAAACGGCAACCTGGCAGCCGAGCGCGTCCATCCCCAATTTATTAAAACGTGCGGCGATTATGGCGGAAATCCG
TCGTTTCTTTGCCGATCGTGGAGTGCTTGAGGTTGAGACGCCCTGCATGAGTCAGGCGACGGTCACAGACATTCATCTGT
TCCCGTTCGAAACGCGTTTCGTCGGACCTGGCCATTCCCAGGGGATCAACCTCTATTTAATGACCAGTCCGGAATACCAT
ATGAAACGCCTGCTGGCGGCAGGGTGCGGTCCGGTTTTCCAGCTATGCCGCAGTTTCCGTAATGAAGAGATGGGACGACA
TCATAATCCGGAATTCACCATGCTGGAGTGGTATCGCCCGCATTACGATATGTACCGCCTGATGAATGAAGTGGATGATT
TGCTTCAGCAAGTGCTGGATTGTCAACCTGCGGAAAGTCTCTCCTATCAACAGGCGTTTCTGCGCCATCTGGAGATTGAC
CCGTTATCAGCAGATAAAACGCAGCTGCGTGAGGCGGCGGCAAAGCTTGATTTAAGCAATATCGCCGATACGGAAGAAGA
CCGTGATACGTTGCTGCAACTGCTGTTCACGATGGGGGTTGAGCCGCATATAGGTAAAGAAAAGCCGACCTTTATTTATC
ACTTTCCGGCCAGTCAGGCATCGCTGGCACAAATCAGTACCGAGGATCATCGCGTCGCCGAGCGCTTTGAGGTGTACTAC
AAAGGTATTGAGCTGGCGAATGGTTTCCACGAACTGACGGACGCACGTGAGCAACAACAGCGCTTTGAACAGGACAATCG
TAAGCGCGCCGCGCGCGGTCTGCCGCAGCAGCCGATTGACCAAAATCTACTGGATGCGCTGGCCGCCGGTCTACCGGATT
GTTCCGGCGTGGCGCTGGGTGTTGATCGTCTGGTGATGCTGGCGCTGGGAGCAGAAAGCCTGGCGGACGTGATTGCTTTT
ACGGTCGATCGGGCGTAA

Upstream 100 bases:

>100_bases
TTTACCCTCCAGAAGGCACCCGATACGCCTGCGCAAAATTTGTTTCGCCCGCGCGTTGCGAGTAGACTTCGTGACCTTGT
CTTAAACTGGAGAAAGAATC

Downstream 100 bases:

>100_bases
ATCTGAAATTCACTCTTTCGCGAGATAAAATGGCGAAATAAGCGCCATTTTGTAGCATATTTTTTCAATTATCCTCTGTT
TGCCACAACATAAGGCTGGA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSETATWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLFPFETRFVGPGHSQGINLYLMTSPEYH
MKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCQPAESLSYQQAFLRHLEID
PLSADKTQLREAAAKLDLSNIADTEEDRDTLLQLLFTMGVEPHIGKEKPTFIYHFPASQASLAQISTEDHRVAERFEVYY
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQQPIDQNLLDALAAGLPDCSGVALGVDRLVMLALGAESLADVIAF
TVDRA

Sequences:

>Translated_325_residues
MSETATWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLFPFETRFVGPGHSQGINLYLMTSPEYH
MKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCQPAESLSYQQAFLRHLEID
PLSADKTQLREAAAKLDLSNIADTEEDRDTLLQLLFTMGVEPHIGKEKPTFIYHFPASQASLAQISTEDHRVAERFEVYY
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQQPIDQNLLDALAAGLPDCSGVALGVDRLVMLALGAESLADVIAF
TVDRA
>Mature_324_residues
SETATWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLFPFETRFVGPGHSQGINLYLMTSPEYHM
KRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCQPAESLSYQQAFLRHLEIDP
LSADKTQLREAAAKLDLSNIADTEEDRDTLLQLLFTMGVEPHIGKEKPTFIYHFPASQASLAQISTEDHRVAERFEVYYK
GIELANGFHELTDAREQQQRFEQDNRKRAARGLPQQPIDQNLLDALAAGLPDCSGVALGVDRLVMLALGAESLADVIAFT
VDRA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=334, Percent_Identity=27.5449101796407, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI5031815, Length=334, Percent_Identity=27.5449101796407, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=92.9230769230769, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=323, Percent_Identity=32.8173374613003, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789256, Length=320, Percent_Identity=31.25, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71994340, Length=334, Percent_Identity=29.0419161676647, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17535925, Length=334, Percent_Identity=29.0419161676647, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17535927, Length=334, Percent_Identity=29.0419161676647, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320242, Length=332, Percent_Identity=27.710843373494, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324256, Length=347, Percent_Identity=26.5129682997118, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640849, Length=335, Percent_Identity=27.4626865671642, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640851, Length=335, Percent_Identity=27.4626865671642, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 36841; Mature: 36710

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETATWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLFPFETRF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCEE
VGPGHSQGINLYLMTSPEYHMKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRP
CCCCCCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHCC
HYDMYRLMNEVDDLLQQVLDCQPAESLSYQQAFLRHLEIDPLSADKTQLREAAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHH
IADTEEDRDTLLQLLFTMGVEPHIGKEKPTFIYHFPASQASLAQISTEDHRVAERFEVYY
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQQPIDQNLLDALAAGLPDCSGVALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
VDRLVMLALGAESLADVIAFTVDRA
HHHHHHHHHCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETATWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLFPFETRF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEEEEEECCCCEE
VGPGHSQGINLYLMTSPEYHMKRLLAAGCGPVFQLCRSFRNEEMGRHHNPEFTMLEWYRP
CCCCCCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHCC
HYDMYRLMNEVDDLLQQVLDCQPAESLSYQQAFLRHLEIDPLSADKTQLREAAAKLDLSN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHH
IADTEEDRDTLLQLLFTMGVEPHIGKEKPTFIYHFPASQASLAQISTEDHRVAERFEVYY
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHH
KGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQQPIDQNLLDALAAGLPDCSGVALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
VDRLVMLALGAESLADVIAFTVDRA
HHHHHHHHHCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA