Click here to switch to the map view.

The map label for this gene is nrfF [H]

Identifier: 29144450

GI number: 29144450

Start: 4351326

End: 4351838

Strand: Direct

Name: nrfF [H]

Synonym: t4188

Alternate gene names: 29144450

Gene position: 4351326-4351838 (Clockwise)

Preceding gene: 29144449

Following gene: 29144451

Centisome position: 90.8

GC content: 55.56

Gene sequence:

>513_bases
GTGATGCGTAAATTACTCACAGCGTTGCCGGATGGCGGCGTAAGCGCCTTATCCGGCCTACAGGATGCGCAAACCGTAGG
CCGGATTAGCGTAGCGCCATCCGGCAGAAAGTTTTTACTCTTAACGATCGTCCTGTTTTTAATCACATTCGCCACCCACG
CCCAGGTGGTGGATACCTGGCGCTTTGACAATCCACAACAGCAGGAAAAAGCGCTGTCTATTGCCGGTCAACTGCGCTGT
CCGCAGTGTCAGAACCAGAATTTGCTGGAATCCAATGCGCCCGTGGCGGTCAGTATGCGCCATCAGGTTTACAGCATGGT
GGCCGAAGGTAAAAGCGAAGCGGAAATCACCGCCTGGATGACCGACCGTTATGGTGATTTCGTGCGCTACAACCCGCCGT
TGAATGAGCAAACGCTGCTGTTGTGGGCGCTGCCGTGCCTGCTGTTATTGTTGGTGGGCGTGGTGGTCTGGCGGGTGAGA
AAGCGTCAGCACGCAGAAGAGGGGGAACAATGA

Upstream 100 bases:

>100_bases
GCCATGCGCTTATACGTGCAGACCGGCGTGCGCTGGATCTGGGGAGGGGGATTGTTGATGGTGTGTGGCGCGCTGCTGAG
CGGATGGCGGGGGAGGAAGC

Downstream 100 bases:

>100_bases
GCCAGTCTGAGCACTCCACTGTGCCGTTACGTCCCATGCCCGTAAAGCGTCTGACGGCTGCGGCGGTGTTGATGGTGGCT
GTCTGTATCGGCGGCTATAT

Product: formate-dependent nitrite reductase complex subunit NrfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MMRKLLTALPDGGVSALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTWRFDNPQQQEKALSIAGQLRC
PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRVR
KRQHAEEGEQ

Sequences:

>Translated_170_residues
MMRKLLTALPDGGVSALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTWRFDNPQQQEKALSIAGQLRC
PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRVR
KRQHAEEGEQ
>Mature_170_residues
MMRKLLTALPDGGVSALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTWRFDNPQQQEKALSIAGQLRC
PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWMTDRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRVR
KRQHAEEGEQ

Specific function: Not required for the biosynthesis of any of the c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG3088

COG function: function code O; Uncharacterized protein involved in biosynthesis of c-type cytochromes

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmH/CycL/Ccl2/NrfF family [H]

Homologues:

Organism=Escherichia coli, GI1790512, Length=91, Percent_Identity=81.3186813186813, Blast_Score=153, Evalue=7e-39,
Organism=Escherichia coli, GI1788522, Length=119, Percent_Identity=36.9747899159664, Blast_Score=101, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005616
- InterPro:   IPR017565 [H]

Pfam domain/function: PF03918 CcmH [H]

EC number: NA

Molecular weight: Translated: 19030; Mature: 19030

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKLLTALPDGGVSALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTW
CHHHHHHHCCCCCHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RFDNPQQQEKALSIAGQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWM
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCEEEEH
TDRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRVRKRQHAEEGEQ
HHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMRKLLTALPDGGVSALSGLQDAQTVGRISVAPSGRKFLLLTIVLFLITFATHAQVVDTW
CHHHHHHHCCCCCHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RFDNPQQQEKALSIAGQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEAEITAWM
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCCEEEEH
TDRYGDFVRYNPPLNEQTLLLWALPCLLLLLVGVVVWRVRKRQHAEEGEQ
HHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]