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The map label for this gene is nrfE [H]

Identifier: 29144449

GI number: 29144449

Start: 4349600

End: 4351336

Strand: Direct

Name: nrfE [H]

Synonym: t4187

Alternate gene names: 29144449

Gene position: 4349600-4351336 (Clockwise)

Preceding gene: 29144448

Following gene: 29144450

Centisome position: 90.77

GC content: 59.41

Gene sequence:

>1737_bases
TTGCATAATCTGGACATCTTCCTTCCTGAAGCCGGATTTCTGGCGCTGTTGTTGAGTCTCGGGGTCAACGTGTTAACCCC
GCTGGCGACGTGGTGCGGGATGGCGCAGCATTGGCGTGGCGTCATGCGCCTGACGACCTGCGGCGCGTGGACGGCGTTCA
CGCTGCTGCTACTGGCGTTTGCGATTCTGTTTAGCTGTTTTCTGACCAGCGATTTTTCGGTCGTTTATGTTGCGCAGCAT
AGCCATAGTCAGTTGTCATGGGGACTGAAACTGGCGGCGGTATGGGGCGGTCATGAAGGCTCGCTCCTGCTGTGGGCGCT
GCTTCTTTCGGGGTGGACGGTGCTATTCGCCTGGCGCTCCCGGCATGAATCTGACGCGCTTTTTCCGCTGACCCTGAGCA
TCTTGTCCCTCATTATGGCTTCGCTGCTGCTATTTATTGTGCTGTGGTCCGATCCGTTCCTGCGTATTTTCCCTCCCGCC
ATGGAAGGGCGCGACCTTAATCCCATGCTGCAACATCTGGGGCTGATCTTACATCCGCCGCTGCTCTATCTGGGTTATGG
CGGCCTGATGACCGCGGCGAGCGTAGCGCTGGCCTCGCTACTGTGCGGCGGCTTTAACGCGGCAACCGCCTGGGTGTGCT
GGCGCTGGGCGTTACTCGGCTGGTGCGCTCTGACGCTGGGGATCATTCTCGGATCGTGGTGGGCGTATTGCGAACTGGGC
TGGGGCGGCTGGTGGTTCTGGGACCCGGTGGAAAATGCCTCATTGTTGCCGTGGCTTGCCGCCAGCGCGTTGCTGCACAG
TCTGTATGTCAGCCGCCAGCGCGGGAGCTTCCGCCACTGGTCGTTGCTGCTGGCGATACTGACGCTGATCCTCTCGTTGC
TGGGAACGCTGATTGTGCGTTCCGGCATTCTGCTCTCCGTCCATGCTTTTGCGTTAGATAATGTGCGCGCGGTGCCGCTG
TTTATCCTTTTTGCCGCGTTAAGTCTGGCATCGCTTGCTCTGTACGGCTGGCGGGCACGGGAGCCGTATCCGGCGACGCG
TTTGGGCGGCGGAAAGTGCGAAACGCTCATTCTGGCGACGCTGCTGCTCTTTAGCGCCGTCTTGTTGATCGTGCTGGTCG
GGACGCTCTACCCCATGATTTATGGTTTGATGGGCTGGGGAAGGCTTTCGGTGGGCGCGCCCTATTTTAATCGCGTCACG
CTGCCGTTTGGCCTGCTGATGCTGGTGGTTATCGTGCTTGCGACAATCAGAAGCCGCAAAGTCTCTGTGCATCGTCAGCT
TCCGGCGTTGCTGGCGCATACGGGCGTATTTATCTTCGCCGCCGGGATCATGGTTTCCAGCGGCAGTCGGCATGAAATCA
GCCTTAACCTCAGCCCGGGGCAGCAGGTGGTACTGGCGGGCTATACCTTTCGTTTCGAGCGTCTGGATCTGGAAGCCAAA
GGCAACTACACCAGTGAAAAAGCGCGGATCACGTTGTGGCGGAATGAAAAACGCATTGGCAGCCTGCAGCCTGAAAGGCG
CTTCTATGCCGCGCGTCGTCAACAGATGATGGAGCCGGGGATTCACTGGAACCTGCTACATGACTGGTACGCGGTGATGG
GCGAAAAAACCGGAGCGGACCGTTACGCCATGCGCTTATACGTGCAGACCGGCGTGCGCTGGATCTGGGGAGGGGGATTG
TTGATGGTGTGTGGCGCGCTGCTGAGCGGATGGCGGGGGAGGAAGCGTGATGCGTAA

Upstream 100 bases:

>100_bases
CGCAGCGTCACGTTTCACGGCGTGCTGGCCGTTTGCGGCGCCAGTTTGACCGGCGTGCTGCTGCTGCGCTTTTTCATTCT
TTATGCGGGACAGCTAACGG

Downstream 100 bases:

>100_bases
ATTACTCACAGCGTTGCCGGATGGCGGCGTAAGCGCCTTATCCGGCCTACAGGATGCGCAAACCGTAGGCCGGATTAGCG
TAGCGCCATCCGGCAGAAAG

Product: heme lyase subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMAQHWRGVMRLTTCGAWTAFTLLLLAFAILFSCFLTSDFSVVYVAQH
SHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTVLFAWRSRHESDALFPLTLSILSLIMASLLLFIVLWSDPFLRIFPPA
MEGRDLNPMLQHLGLILHPPLLYLGYGGLMTAASVALASLLCGGFNAATAWVCWRWALLGWCALTLGIILGSWWAYCELG
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVRSGILLSVHAFALDNVRAVPL
FILFAALSLASLALYGWRAREPYPATRLGGGKCETLILATLLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVT
LPFGLLMLVVIVLATIRSRKVSVHRQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGADRYAMRLYVQTGVRWIWGGGL
LMVCGALLSGWRGRKRDA

Sequences:

>Translated_578_residues
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMAQHWRGVMRLTTCGAWTAFTLLLLAFAILFSCFLTSDFSVVYVAQH
SHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTVLFAWRSRHESDALFPLTLSILSLIMASLLLFIVLWSDPFLRIFPPA
MEGRDLNPMLQHLGLILHPPLLYLGYGGLMTAASVALASLLCGGFNAATAWVCWRWALLGWCALTLGIILGSWWAYCELG
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVRSGILLSVHAFALDNVRAVPL
FILFAALSLASLALYGWRAREPYPATRLGGGKCETLILATLLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVT
LPFGLLMLVVIVLATIRSRKVSVHRQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGADRYAMRLYVQTGVRWIWGGGL
LMVCGALLSGWRGRKRDA
>Mature_578_residues
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMAQHWRGVMRLTTCGAWTAFTLLLLAFAILFSCFLTSDFSVVYVAQH
SHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTVLFAWRSRHESDALFPLTLSILSLIMASLLLFIVLWSDPFLRIFPPA
MEGRDLNPMLQHLGLILHPPLLYLGYGGLMTAASVALASLLCGGFNAATAWVCWRWALLGWCALTLGIILGSWWAYCELG
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVRSGILLSVHAFALDNVRAVPL
FILFAALSLASLALYGWRAREPYPATRLGGGKCETLILATLLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVT
LPFGLLMLVVIVLATIRSRKVSVHRQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGADRYAMRLYVQTGVRWIWGGGL
LMVCGALLSGWRGRKRDA

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=554, Percent_Identity=77.0758122743682, Blast_Score=779, Evalue=0.0,
Organism=Escherichia coli, GI1788524, Length=634, Percent_Identity=37.3817034700315, Blast_Score=343, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 64321; Mature: 64321

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMAQHWRGVMRLTTCGAWTAFTLLLLAF
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHH
AILFSCFLTSDFSVVYVAQHSHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTVLFAWRS
HHHHHHHHCCCCCEEEEEECCCCHHHHCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHH
RHESDALFPLTLSILSLIMASLLLFIVLWSDPFLRIFPPAMEGRDLNPMLQHLGLILHPP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHCHHHHCCH
LLYLGYGGLMTAASVALASLLCGGFNAATAWVCWRWALLGWCALTLGIILGSWWAYCELG
HHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEC
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVR
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SGILLSVHAFALDNVRAVPLFILFAALSLASLALYGWRAREPYPATRLGGGKCETLILAT
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
LLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVTLPFGLLMLVVIVLATIRSRK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VSVHRQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEEEEEEEEEEC
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGAD
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
RYAMRLYVQTGVRWIWGGGLLMVCGALLSGWRGRKRDA
HHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHNLDIFLPEAGFLALLLSLGVNVLTPLATWCGMAQHWRGVMRLTTCGAWTAFTLLLLAF
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHH
AILFSCFLTSDFSVVYVAQHSHSQLSWGLKLAAVWGGHEGSLLLWALLLSGWTVLFAWRS
HHHHHHHHCCCCCEEEEEECCCCHHHHCEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHH
RHESDALFPLTLSILSLIMASLLLFIVLWSDPFLRIFPPAMEGRDLNPMLQHLGLILHPP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHCHHHHCCH
LLYLGYGGLMTAASVALASLLCGGFNAATAWVCWRWALLGWCALTLGIILGSWWAYCELG
HHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEC
WGGWWFWDPVENASLLPWLAASALLHSLYVSRQRGSFRHWSLLLAILTLILSLLGTLIVR
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SGILLSVHAFALDNVRAVPLFILFAALSLASLALYGWRAREPYPATRLGGGKCETLILAT
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
LLLFSAVLLIVLVGTLYPMIYGLMGWGRLSVGAPYFNRVTLPFGLLMLVVIVLATIRSRK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VSVHRQLPALLAHTGVFIFAAGIMVSSGSRHEISLNLSPGQQVVLAGYTFRFERLDLEAK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEEEEEEEEEEEC
GNYTSEKARITLWRNEKRIGSLQPERRFYAARRQQMMEPGIHWNLLHDWYAVMGEKTGAD
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
RYAMRLYVQTGVRWIWGGGLLMVCGALLSGWRGRKRDA
HHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]