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The map label for this gene is kefB [H]

Identifier: 29144313

GI number: 29144313

Start: 4205136

End: 4206941

Strand: Direct

Name: kefB [H]

Synonym: t4048

Alternate gene names: 29144313

Gene position: 4205136-4206941 (Clockwise)

Preceding gene: 29144312

Following gene: 29144314

Centisome position: 87.75

GC content: 54.98

Gene sequence:

>1806_bases
ATGGAAGGTGCTGATTTATTGACCGCGGGAGTGTTGTTCCTCTTCGCGGCGGTAGCGGCGGTGCCGCTGGCCGCGAGGCT
GGGCATCGGCGCGGTGCTAGGGTATTTGCTGGCGGGAATCGCCATCGGTCCGTGGGGGCTGGGATTTATCAGCGATGTTG
ACGAGATCCTGCACTTTTCCGAACTGGGCGTCGTGTTTCTGATGTTTATCATTGGTCTCGAACTCAATCCGTCACGGCTT
TGGCAACTGCGGCGCTCTATCTTCGGCGTCGGCGCTGCTCAGGTGCTGTTAAGCGCGGCGGTGCTGGCGGGATTATTGAT
GCTTGCTGATTTCTTATGGCAGGCGGCGGTCGTCGGCGGGATTGGCCTGGCGATGTCTTCGACGGCGATGGCGCTACAAC
TGATGCGCGAGAAAGGCATGAATCGTAGCGAGTCCGGGCAGTTAGGGTTTTCCGTCTTACTGTTTCAGGATTTAGCGGTG
ATCCCCGCGCTGGCGTTGGTGCCTTTATTGGCGGGATCGGCCGACGAGCATTTCGACTGGTTTAAAGTGGCAATGAAGGT
ACTGGCTTTCGCGGTAATGCTGATTGGCGGTCGTTACCTGCTGCGCCCGGTATTTCGTTTTATTGCTGCGTCTGGCGTGC
GGGAGGTCTTCACTGCCGCGACGCTGTTGCTGGTGTTGAGCGCCGCCTTATTTATGGATGCGCTGGGACTGTCGATGGCC
CTGGGAACCTTCATTGCCGGCGTTTTACTGGCAGAGAGCGAATACCGGCATGAGCTGGAAAATGCTATCGATCCCTTTAA
AGGTTTACTGCTTGGGCTGTTCTTTATCTCCGTCGGTATGTCGCTAAATCTCGGCGTACTTTATACCCATTTACTGTGGG
TGGCGGCGAGCGTTGTTATTCTGGTCGTCATAAAAATGCTGACGCTGTATCTGCTGGCGCGGCTATATGGAATAAGAAGT
TCTGAACGGATGCAGTTTGCCAGCGTGCTGAGTCAGGGGGGCGAGTTCGCTTTTGTGCTCTTTTCTACCGCCTCTTCTCA
GCGCCTGTTTCAGGGCGATCAGATGGCGTTATTATTGGTGACTGTCACATTATCAATGATGACCACGCCGCTACTGATGA
AAGGGATTGATAAGTGGTTATCGCGCCGTCTTAATGGCCCGGAAGAGAATGATGAAAAGCCGTGGGTTGAGGATGATAAG
CCCCAGGTGGTTGTCGTCGGCTTTGGCCGTTTTGGTCAGGTGATCGCTCGCTTATTGATGGCGAATAAGATGCGTATCAC
CGTGCTGGAACGCGATATCGGCGCCGTTAATCTGATGCGCAAATATGGTTATAAAGTGTATTACGGCGACGCTACACAAG
TCGAGCTGCTGCGTTCTGCGGGCGCGGAGGCGGCGGAGTCGATAGTCATCACGTGTAATGAACCGGAAGACACGATGAAG
CTGGTGGCGCTGTGTCAGCAGCATTTTCCTCATCTGCATATTCTTGCGCGAGCGCGTGGACGTGTGGAGGCCCATGAGTT
ATTACAGGCTGGCGTCACGCAGTTTTCCCGTGAAACGTTTTCCAGCGCGCTGGAACTGGGACGTAAAACTCTGGTGTCGT
TAGGTATGCATCCGCATCAGGCGCAACGCGCGCAGTTGCATTTTCGTCGGCTGGATATGCGAATGTTGCGGGAGCTGATC
CCGGAGCATAGCGACATGGTACAGATTTCGCGCGCCAGAGAGGCCCGACGCGAGCTGGAGGAGATTTTTCAGCGCGAAAT
GCAACAGGAACGGCGCCAGTTGGATGGCTGGGATGAATTTGAGTAG

Upstream 100 bases:

>100_bases
TTGTGTACTGGGCGCGCCGCCAGTCGCCGCAGACGCTGGCCAGTCATGCGAAAGCCTATGGCGAGTGGCTGGCGAATCCT
GTGAGCGCGGGAGGCTATTG

Downstream 100 bases:

>100_bases
AGGTGAACAATGGCAATCCGTAAACGTTTTATTGCAGGCGCGAAATGTCCGGCCTGTCAGGCGCAGGATACAATGGCGAT
GTGGCGTGAAAATAATGTCG

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MEGADLLTAGVLFLFAAVAAVPLAARLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSRL
WQLRRSIFGVGAAQVLLSAAVLAGLLMLADFLWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWFKVAMKVLAFAVMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLSAALFMDALGLSMA
LGTFIAGVLLAESEYRHELENAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVAASVVILVVIKMLTLYLLARLYGIRS
SERMQFASVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKGIDKWLSRRLNGPEENDEKPWVEDDK
PQVVVVGFGRFGQVIARLLMANKMRITVLERDIGAVNLMRKYGYKVYYGDATQVELLRSAGAEAAESIVITCNEPEDTMK
LVALCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVSLGMHPHQAQRAQLHFRRLDMRMLRELI
PEHSDMVQISRAREARRELEEIFQREMQQERRQLDGWDEFE

Sequences:

>Translated_601_residues
MEGADLLTAGVLFLFAAVAAVPLAARLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSRL
WQLRRSIFGVGAAQVLLSAAVLAGLLMLADFLWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWFKVAMKVLAFAVMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLSAALFMDALGLSMA
LGTFIAGVLLAESEYRHELENAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVAASVVILVVIKMLTLYLLARLYGIRS
SERMQFASVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKGIDKWLSRRLNGPEENDEKPWVEDDK
PQVVVVGFGRFGQVIARLLMANKMRITVLERDIGAVNLMRKYGYKVYYGDATQVELLRSAGAEAAESIVITCNEPEDTMK
LVALCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVSLGMHPHQAQRAQLHFRRLDMRMLRELI
PEHSDMVQISRAREARRELEEIFQREMQQERRQLDGWDEFE
>Mature_601_residues
MEGADLLTAGVLFLFAAVAAVPLAARLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPSRL
WQLRRSIFGVGAAQVLLSAAVLAGLLMLADFLWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHFDWFKVAMKVLAFAVMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLSAALFMDALGLSMA
LGTFIAGVLLAESEYRHELENAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVAASVVILVVIKMLTLYLLARLYGIRS
SERMQFASVLSQGGEFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKGIDKWLSRRLNGPEENDEKPWVEDDK
PQVVVVGFGRFGQVIARLLMANKMRITVLERDIGAVNLMRKYGYKVYYGDATQVELLRSAGAEAAESIVITCNEPEDTMK
LVALCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVSLGMHPHQAQRAQLHFRRLDMRMLRELI
PEHSDMVQISRAREARRELEEIFQREMQQERRQLDGWDEFE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=382, Percent_Identity=26.1780104712042, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1789749, Length=601, Percent_Identity=93.3444259567388, Blast_Score=1074, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=604, Percent_Identity=42.8807947019868, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1786685, Length=527, Percent_Identity=30.7400379506641, Blast_Score=192, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66389; Mature: 66389

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGADLLTAGVLFLFAAVAAVPLAARLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ELGVVFLMFIIGLELNPSRLWQLRRSIFGVGAAQVLLSAAVLAGLLMLADFLWQAAVVGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDW
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FKVAMKVLAFAVMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLSAALFMDALGLSMA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFIAGVLLAESEYRHELENAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVAASVVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LVVIKMLTLYLLARLYGIRSSERMQFASVLSQGGEFAFVLFSTASSQRLFQGDQMALLLV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCHHHHHHH
TVTLSMMTTPLLMKGIDKWLSRRLNGPEENDEKPWVEDDKPQVVVVGFGRFGQVIARLLM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
ANKMRITVLERDIGAVNLMRKYGYKVYYGDATQVELLRSAGAEAAESIVITCNEPEDTMK
HCCCEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCHHHCCEEEEECCCHHHHH
LVALCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVSLGMHPHQ
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AQRAQLHFRRLDMRMLRELIPEHSDMVQISRAREARRELEEIFQREMQQERRQLDGWDEF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
E
C
>Mature Secondary Structure
MEGADLLTAGVLFLFAAVAAVPLAARLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ELGVVFLMFIIGLELNPSRLWQLRRSIFGVGAAQVLLSAAVLAGLLMLADFLWQAAVVGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDW
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FKVAMKVLAFAVMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLSAALFMDALGLSMA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFIAGVLLAESEYRHELENAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVAASVVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LVVIKMLTLYLLARLYGIRSSERMQFASVLSQGGEFAFVLFSTASSQRLFQGDQMALLLV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCHHHHHHH
TVTLSMMTTPLLMKGIDKWLSRRLNGPEENDEKPWVEDDKPQVVVVGFGRFGQVIARLLM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
ANKMRITVLERDIGAVNLMRKYGYKVYYGDATQVELLRSAGAEAAESIVITCNEPEDTMK
HCCCEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCHHHCCEEEEECCCHHHHH
LVALCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVSLGMHPHQ
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AQRAQLHFRRLDMRMLRELIPEHSDMVQISRAREARRELEEIFQREMQQERRQLDGWDEF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA