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The map label for this gene is gph [H]

Identifier: 29144292

GI number: 29144292

Start: 4177240

End: 4177998

Strand: Direct

Name: gph [H]

Synonym: t4025

Alternate gene names: 29144292

Gene position: 4177240-4177998 (Clockwise)

Preceding gene: 29144291

Following gene: 29144293

Centisome position: 87.17

GC content: 55.6

Gene sequence:

>759_bases
ATGAATAAGTTGCAGAATATTCGGGGCGTCGCCTTTGATCTTGACGGTACGCTGGTGGATAGCGCGCCGGGTCTTGCCGC
GGCGGTGGATATGGCGCTGTATGCGCTGGAACTGCCGGTCGCGGGCGAGGAGCGCGTGATTACCTGGATTGGTAACGGCG
CAGACGTATTGATGGAACGCGCGCTGACCTGGGCTAGCCAAGAGCGCGCCACGCTGCGTAAGACTATGGGGAAACCGCCC
GTTGATGAAGATATTCCTGCCGAGGAACAGGTACGCATTCTGCGTAAACTGTTCGACAGGTATTATGGCGAAGTGGCGGA
AGAGGGCACCTTTTTATTTCCGCATGTCGCCGACACGCTGGGCGCGCTGCACGCCAGCGGATTGTCATTAGGTCTGGTGA
CGAATAAGCCGACGCCGTTCGTCGCGCCGTTGCTGGAATCGCTTGATATCGCCAAATACTTTAGCGTGGTTATCGGCGGC
GATGATGTGCAAAATAAGAAGCCGCATCCAGAGCCGTTGTTGCTGGTGGCAAGCCGACTGGGCATGACGCCGGAGCAGAT
GCTTTTTGTCGGCGATTCGCGTAATGATATTCAGGCCGCAAAAGCGGCGGGCTGCCCTTCGGTTGGCCTGACATACGGCT
ACAATTATGGCGAAGTGATCGCCCTTAGCGAGCCGGACGTCATTTATGACAGTTTTAACGATCTTTTGCCCGCACTTGGG
CTTCCGCATAGCGATAACCAGGAAATAAAAAATGACTAA

Upstream 100 bases:

>100_bases
GCTGGTGCGGACATGTTTGTTGCGGGGTCGGCAATTTTCGATAAACCCGACTACAAAAAAGTGATTGATGAAATGCGCAG
TGAGCTGGCAAAGGTAAGTC

Downstream 100 bases:

>100_bases
GCCCATCGTTTTTAGTGGCGCACAGCCCTCAGGTGAATTGACCATTGGCAACTATATGGGGGCATTGCGTCAGTGGGTAA
ACATGCAGGATGACTATCAT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWASQERATLRKTMGKPP
VDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGG
DDVQNKKPHPEPLLLVASRLGMTPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEVIALSEPDVIYDSFNDLLPALG
LPHSDNQEIKND

Sequences:

>Translated_252_residues
MNKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWASQERATLRKTMGKPP
VDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGG
DDVQNKKPHPEPLLLVASRLGMTPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEVIALSEPDVIYDSFNDLLPALG
LPHSDNQEIKND
>Mature_252_residues
MNKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMERALTWASQERATLRKTMGKPP
VDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTLGALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGG
DDVQNKKPHPEPLLLVASRLGMTPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEVIALSEPDVIYDSFNDLLPALG
LPHSDNQEIKND

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=252, Percent_Identity=90.8730158730159, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1787576, Length=219, Percent_Identity=23.7442922374429, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 27278; Mature: 27278

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCCCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
ALTWASQERATLRKTMGKPPVDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGGDDVQNKKPHPEPLLLVASRL
HHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHH
GMTPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEVIALSEPDVIYDSFNDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHHHC
LPHSDNQEIKND
CCCCCCCCCCCC
>Mature Secondary Structure
MNKLQNIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEERVITWIGNGADVLMER
CCCCCCCCCEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
ALTWASQERATLRKTMGKPPVDEDIPAEEQVRILRKLFDRYYGEVAEEGTFLFPHVADTL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHH
GALHASGLSLGLVTNKPTPFVAPLLESLDIAKYFSVVIGGDDVQNKKPHPEPLLLVASRL
HHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHH
GMTPEQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGEVIALSEPDVIYDSFNDLLPALG
CCCHHHEEEECCCCCHHHHHHHCCCCCCCCEECCCCCCEEEECCCCHHHHHHHHHHHHHC
LPHSDNQEIKND
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]