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The map label for this gene is dam [H]

Identifier: 29144290

GI number: 29144290

Start: 4175716

End: 4176552

Strand: Direct

Name: dam [H]

Synonym: t4022

Alternate gene names: 29144290

Gene position: 4175716-4176552 (Clockwise)

Preceding gene: 29144289

Following gene: 29144291

Centisome position: 87.14

GC content: 49.22

Gene sequence:

>837_bases
ATGAAAAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGAAAATACCCTCTGCTTGATGATATCAAACGACATTTGCCAAA
AGGCGAGTGCCTTGTCGAACCTTTTGTGGGTGCCGGATCGGTGTTTCTTAACACCGACTTTTCTCGTTATATCCTCGCCG
ATATCAACAGCGACCTTATTAGCCTCTATAACATCGTGAAGTTACGTACTGACGAGTATGTACAGGCTTCGCGCGAGCTG
TTTATGCCTGAAACTAACCAGGCTGAGGTCTATTACCAACTCCGTGAGGAGTTCAACACCTGCCAGGACCCCTTTCGTCG
GGCGGTACTGTTTTTATATCTGAACCGCTACGGTTATAACGGACTCTGCCGCTATAACTTGCGCGGGGAATTTAATGTAC
CGTTTGGTCGTTATAAAAGACCTTACTTCCCGGAAGCGGAGTTGTACCACTTTGCAGAAAAAGCGCAGAATGCGTTCTTT
TACTGTGAGTCATACGCAGACAGTATGGCGCGTGCGGACAAGTCGTCGGTGGTCTATTGCGATCCGCCTTATGCGCCGTT
GTCCGCCACGGCTAACTTCACAGCGTATCACACCAATAGTTTTAGCCTGACGCAACAAGCGCATCTGGCGGAAATCGCTG
AGAATCTGGTCAGTAACCGGATACCGGTGCTCATTTCCAACCATGATACGGCGCTCACACGCGAGTGGTATCAACTCGCA
AAATTGCATGTCGTCAAAGTTAGACGCAGTATAAGCAGCAACGGCGGCACACGTAAAAAGGTGGACGAACTGCTGGCTCT
GTACCAACCAGGAGTCGCAACGCCTGCAAGAAAATAA

Upstream 100 bases:

>100_bases
TACCGTCAAATGCGCCGTAAAGCAACGCGGCACAGCCTAAAGCGCAGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAA
GGTGTAATTAGTTAGTCAGC

Downstream 100 bases:

>100_bases
TTCTCAAGGAGAAGCGGATGAAACAGTATTTGATTGCCCCCTCAATTCTGTCGGCTGATTTTGCTCGCCTGGGTGAAGAC
ACCGCCAAAGCGCTGGCTGC

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKLRTDEYVQASREL
FMPETNQAEVYYQLREEFNTCQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKRPYFPEAELYHFAEKAQNAFF
YCESYADSMARADKSSVVYCDPPYAPLSATANFTAYHTNSFSLTQQAHLAEIAENLVSNRIPVLISNHDTALTREWYQLA
KLHVVKVRRSISSNGGTRKKVDELLALYQPGVATPARK

Sequences:

>Translated_278_residues
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKLRTDEYVQASREL
FMPETNQAEVYYQLREEFNTCQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKRPYFPEAELYHFAEKAQNAFF
YCESYADSMARADKSSVVYCDPPYAPLSATANFTAYHTNSFSLTQQAHLAEIAENLVSNRIPVLISNHDTALTREWYQLA
KLHVVKVRRSISSNGGTRKKVDELLALYQPGVATPARK
>Mature_278_residues
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKLRTDEYVQASREL
FMPETNQAEVYYQLREEFNTCQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKRPYFPEAELYHFAEKAQNAFF
YCESYADSMARADKSSVVYCDPPYAPLSATANFTAYHTNSFSLTQQAHLAEIAENLVSNRIPVLISNHDTALTREWYQLA
KLHVVKVRRSISSNGGTRKKVDELLALYQPGVATPARK

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplica

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=278, Percent_Identity=92.0863309352518, Blast_Score=538, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32028; Mature: 32028

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLI
CCCCCEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCEEEECCCHHHH
SLYNIVKLRTDEYVQASRELFMPETNQAEVYYQLREEFNTCQDPFRRAVLFLYLNRYGYN
HHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLCRYNLRGEFNVPFGRYKRPYFPEAELYHFAEKAQNAFFYCESYADSMARADKSSVVYC
CEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCEEEE
DPPYAPLSATANFTAYHTNSFSLTQQAHLAEIAENLVSNRIPVLISNHDTALTREWYQLA
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
KLHVVKVRRSISSNGGTRKKVDELLALYQPGVATPARK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLI
CCCCCEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCEEEEECCCCCEEEECCCHHHH
SLYNIVKLRTDEYVQASRELFMPETNQAEVYYQLREEFNTCQDPFRRAVLFLYLNRYGYN
HHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLCRYNLRGEFNVPFGRYKRPYFPEAELYHFAEKAQNAFFYCESYADSMARADKSSVVYC
CEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCEEEE
DPPYAPLSATANFTAYHTNSFSLTQQAHLAEIAENLVSNRIPVLISNHDTALTREWYQLA
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
KLHVVKVRRSISSNGGTRKKVDELLALYQPGVATPARK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]