Click here to switch to the map view.

The map label for this gene is livM [H]

Identifier: 29144228

GI number: 29144228

Start: 4099235

End: 4100512

Strand: Direct

Name: livM [H]

Synonym: t3960

Alternate gene names: 29144228

Gene position: 4099235-4100512 (Clockwise)

Preceding gene: 29144227

Following gene: 29144229

Centisome position: 85.54

GC content: 57.75

Gene sequence:

>1278_bases
ATGAAACCGATGCATATTGCGATGGCTCTGTTCTCTGCGGCGATGTTCTTCGTTCTGGCTGGCGTCTTTATGGGCGTACA
ACTGGAGCTGGACGGCACCAAACTGGTGGTGGATACCGCCGCCGACATCCGCTGGCAGTGGATCTTTATCGGTACGGCAG
TGGTTTTTTTCTTTCAGTTGCTGCGTCCGATGTTCCAGAAAGCGGTGAAGCACGTCTCCGGACCGAAGTTTATTCTGCCG
GCGATCGATGGCTCTACCGTTAAGCAAAAGCTGTTTCTGATGGCGCTGTTGGTTATCGCCGTGGCGTGGCCGTTTATGGT
GTCGCGCGGTAGCGTCGATATCGCCACTATGACCATGATTTATATCATCCTCGGTCTGGGGCTGAACGTGGTGGTGGGGC
TGTCCGGCCTGCTGGTATTGGGCTACGGCGGCTTTTACGCCATCGGCGCTTATACCTTTGCGCTGCTTAACCACTATTAC
GGTTTGGGTTTCTGGACCTGCCTGCCGCTGGCGGGACTGGTCTCCGCCGCCGCCGGTTTCCTGCTCGGCTTCCCGGTACT
GCGCCTGCGCGGCGACTATCTGGCGATTGTGACGCTCGGCTTCGGCGAAATCGTCCGTATCCTGTTGCTCAACAACACTG
AAATTACCGGCGGCCCGAACGGCATCAGCCAGATCCCCAAACCGACGCTGTTTGGTCTGGAGTTTAGCCGCAACACCCGA
GAAGGCGGCTGGGATACCTTCAGCAATTTCTTTGGCGTGAAGTACGACCCATCCGACCGGGTCATTTTCCTCTATCTGGT
GGCCCTGTTGCTGGTGGTCCTGTCGCTGTTTGTCATTAACCGCCTGCTGCGTATGCCGCTGGGCCGGGCGTGGGAAGCGT
TGCGTGAAGATGAGATCGCCTGTCGCTCATTAGGTTTAAGCCCGACGCGCATCAAGCTGACCGCTTTTACTATCAGCGCC
GCGTTTGCCGGTTTCGCCGGGACGTTGTTTGCCGCCCGCCAGGGCTTTGTCAGCCCGGAATCCTTCACTTTCGCCGAATC
CGCCTTCGTGCTGGCGATTGTGGTGCTGGGCGGAATGGGCTCGCAGTTTGCGGTGATCCTGGCGGCGGTTCTGTTGGTGG
TGTCGCGCGAGTTGATGCGCGATTTCAACGAATACAGCATGTTGATGCTCGGCGGTTTAATGGTGTTGATGATGATCTGG
CGCCCGCAGGGATTGCTGCCGATGACGCGTCCGCAGTTGAAGCTGAAAAGTGGTCAGGCGAAAGGAGAGCAGGCATGA

Upstream 100 bases:

>100_bases
TAAGTACCGAATATAAAGATGTCGTGTCGTTCGCTCTGCTGATTCTGGTACTGCTGGTGATGCCGACCGGTATCCTGGGG
CGTCCGGAGGTAGAGAAAGT

Downstream 100 bases:

>100_bases
GTCAGCCATTATTAGCGGTTAACGGCCTGATGATGCGCTTTGGCGGTTTGCTGGCGGTCAACAATGTATCGCTGGAATTG
CGTGAGCGGGAAATTGTGTC

Product: leucine/isoleucine/valine transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKPMHIAMALFSAAMFFVLAGVFMGVQLELDGTKLVVDTAADIRWQWIFIGTAVVFFFQLLRPMFQKAVKHVSGPKFILP
AIDGSTVKQKLFLMALLVIAVAWPFMVSRGSVDIATMTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
GLGFWTCLPLAGLVSAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTLFGLEFSRNTR
EGGWDTFSNFFGVKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIACRSLGLSPTRIKLTAFTISA
AFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGGLMVLMMIW
RPQGLLPMTRPQLKLKSGQAKGEQA

Sequences:

>Translated_425_residues
MKPMHIAMALFSAAMFFVLAGVFMGVQLELDGTKLVVDTAADIRWQWIFIGTAVVFFFQLLRPMFQKAVKHVSGPKFILP
AIDGSTVKQKLFLMALLVIAVAWPFMVSRGSVDIATMTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
GLGFWTCLPLAGLVSAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTLFGLEFSRNTR
EGGWDTFSNFFGVKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIACRSLGLSPTRIKLTAFTISA
AFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGGLMVLMMIW
RPQGLLPMTRPQLKLKSGQAKGEQA
>Mature_425_residues
MKPMHIAMALFSAAMFFVLAGVFMGVQLELDGTKLVVDTAADIRWQWIFIGTAVVFFFQLLRPMFQKAVKHVSGPKFILP
AIDGSTVKQKLFLMALLVIAVAWPFMVSRGSVDIATMTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
GLGFWTCLPLAGLVSAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTLFGLEFSRNTR
EGGWDTFSNFFGVKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIACRSLGLSPTRIKLTAFTISA
AFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGGLMVLMMIW
RPQGLLPMTRPQLKLKSGQAKGEQA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=425, Percent_Identity=94.8235294117647, Blast_Score=763, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46436; Mature: 46436

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPMHIAMALFSAAMFFVLAGVFMGVQLELDGTKLVVDTAADIRWQWIFIGTAVVFFFQL
CCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCEEEEEHHHHHHHHHHHH
LRPMFQKAVKHVSGPKFILPAIDGSTVKQKLFLMALLVIAVAWPFMVSRGSVDIATMTMI
HHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
YIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYYGLGFWTCLPLAGLVSAAAGF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTLFGLEFSRNTR
HHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEECCCCC
EGGWDTFSNFFGVKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIA
CCCCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CRSLGLSPTRIKLTAFTISAAFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMG
HHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
SQFAVILAAVLLVVSRELMRDFNEYSMLMLGGLMVLMMIWRPQGLLPMTRPQLKLKSGQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
KGEQA
CCCCC
>Mature Secondary Structure
MKPMHIAMALFSAAMFFVLAGVFMGVQLELDGTKLVVDTAADIRWQWIFIGTAVVFFFQL
CCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCEEEEEHHHHHHHHHHHH
LRPMFQKAVKHVSGPKFILPAIDGSTVKQKLFLMALLVIAVAWPFMVSRGSVDIATMTMI
HHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
YIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYYGLGFWTCLPLAGLVSAAAGF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGGPNGISQIPKPTLFGLEFSRNTR
HHHHHHHHHCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEECCCCC
EGGWDTFSNFFGVKYDPSDRVIFLYLVALLLVVLSLFVINRLLRMPLGRAWEALREDEIA
CCCCHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
CRSLGLSPTRIKLTAFTISAAFAGFAGTLFAARQGFVSPESFTFAESAFVLAIVVLGGMG
HHHCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
SQFAVILAAVLLVVSRELMRDFNEYSMLMLGGLMVLMMIWRPQGLLPMTRPQLKLKSGQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
KGEQA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]