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The map label for this gene is aaeR [H]

Identifier: 29143615

GI number: 29143615

Start: 3372390

End: 3373319

Strand: Direct

Name: aaeR [H]

Synonym: t3282

Alternate gene names: 29143615

Gene position: 3372390-3373319 (Clockwise)

Preceding gene: 29143610

Following gene: 29143624

Centisome position: 70.38

GC content: 53.55

Gene sequence:

>930_bases
ATGGAACGATTAAAACGAATGTCGGTATTCGCCAAAGTTGTTGAGTTTGGCTCTTTTACCGCCGCAGCCAGACAGCTGCA
AATGAGCGTATCGTCCATCAGCCAGACTGTGGCGAAACTGGAAGATGAGCTTCAGGTTAAATTGCTCAACCGCAGCACAC
GCAGCATTGGGCTCACCGAAGCTGGAAAAATCTATTATCAGGGCTGTCGTCGTATGCTGCATGAAGTGCAGGATGTTCAC
GAGCAGCTTTACGCTTTTAATAACACGCCTATCGGTACATTGCGTATCGGCTGTTCTTCAACCATGGCGCAAAATGTGCT
GGCCGGGCTTACCGCAAAATTATTAAAAGAGTATCCCGGTCTGGCGGTCAATCTGGTGACAGGAATTCCGGCCCCGGATT
TAATTGCCGACGGGCTGGACGTGGTGATCCGCGTGGGCGCGCTGCAAGACTCCAGCCTGTTTTCCCGTCGACTCGGCGCG
ATGCCGATGGTTGTGTGCGCCGCCAAGCCCTACCTCGCTCAGTACGGCGTCCCGGAAAAACCCGCCGATCTCAGTAGCCA
TTCCTGGCTGGAATATAGCGTGCGCCCGGATAATGAGTTTGAACTTATTGCGCCGGAAGGAATTTCTACCCGACTGATTC
CACAGGGGCGGTTCGTCACTAACGATCCGATGACGCTGGTACGCTGGCTGACCGCCGGAACCGGAATCGCCTATGTGCCG
CTGATGTGGGTGATCGATGAGATCAATCGTGGCGATCTGGAGATCCTGCTGCCTCGCTATCAGTCCGATCCCCGTCCGGT
GTACGCGCTGTACACCGAAAAAGATAAGCTGCCGCTTAAAGTGCAGGTAGTGATTAACGCGTTAACTGACTATTTTGTCG
ATGTCGCACACCTGTTTCAGGGGATGCACGGGCGAGGGAAAGAAAAATAA

Upstream 100 bases:

>100_bases
AGAGCGACATAGCTCTTATTCGAATAATAGTATATTAACGTAATTGTTATGCTGTTATCTATATTATGTGATCTAAATCA
CTTTTAAGCCAGAGTGAATA

Downstream 100 bases:

>100_bases
AAACACCGGGTAATTGCTGAAGACGATTGTAGATCAGATGCCACCCGAGGCAGACATTGCCTGATGGCGCTGCGCTTATC
AGGCCTGGGCGGCACATACC

Product: DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVAKLEDELQVKLLNRSTRSIGLTEAGKIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKLLKEYPGLAVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKPYLAQYGVPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGTGIAYVP
LMWVIDEINRGDLEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINALTDYFVDVAHLFQGMHGRGKEK

Sequences:

>Translated_309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVAKLEDELQVKLLNRSTRSIGLTEAGKIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKLLKEYPGLAVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKPYLAQYGVPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGTGIAYVP
LMWVIDEINRGDLEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINALTDYFVDVAHLFQGMHGRGKEK
>Mature_309_residues
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVAKLEDELQVKLLNRSTRSIGLTEAGKIYYQGCRRMLHEVQDVH
EQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKLLKEYPGLAVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
MPMVVCAAKPYLAQYGVPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGTGIAYVP
LMWVIDEINRGDLEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINALTDYFVDVAHLFQGMHGRGKEK

Specific function: Activates transcription of the aaeXAB operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789639, Length=309, Percent_Identity=95.1456310679612, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=30.7167235494881, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=31.25, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1787128, Length=266, Percent_Identity=28.9473684210526, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=30.3754266211604, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=27.5862068965517, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=28.4671532846715, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI157672245, Length=174, Percent_Identity=29.8850574712644, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788481, Length=146, Percent_Identity=30.1369863013699, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=257, Percent_Identity=25.6809338521401, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787601, Length=126, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788297, Length=123, Percent_Identity=31.7073170731707, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788706, Length=145, Percent_Identity=28.9655172413793, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786448, Length=143, Percent_Identity=30.0699300699301, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34342; Mature: 34342

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVAKLEDELQVKLLNRSTRSIGLTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCH
AGKIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKLLKEYPG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
LAVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGAMPMVVCAAKPYLAQYGVPEK
CEEEEECCCCCHHHHHCCHHHEEEECCCCCHHHHHHHHCCCCEEECCCCHHHHHCCCCCC
PADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGTGIAYVP
CCCCCCCCEEEEEECCCCCEEEECCCCCCEEECCCCCEECCCHHHHHHHHHCCCCHHHHH
LMWVIDEINRGDLEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINALTDYFVDVAHLFQ
HHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
GMHGRGKEK
CCCCCCCCC
>Mature Secondary Structure
MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVAKLEDELQVKLLNRSTRSIGLTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCH
AGKIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTMAQNVLAGLTAKLLKEYPG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
LAVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGAMPMVVCAAKPYLAQYGVPEK
CEEEEECCCCCHHHHHCCHHHEEEECCCCCHHHHHHHHCCCCEEECCCCHHHHHCCCCCC
PADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRFVTNDPMTLVRWLTAGTGIAYVP
CCCCCCCCEEEEEECCCCCEEEECCCCCCEEECCCCCEECCCHHHHHHHHHCCCCHHHHH
LMWVIDEINRGDLEILLPRYQSDPRPVYALYTEKDKLPLKVQVVINALTDYFVDVAHLFQ
HHHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
GMHGRGKEK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]