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The map label for this gene is nanT [H]

Identifier: 29143588

GI number: 29143588

Start: 3346518

End: 3348008

Strand: Reverse

Name: nanT [H]

Synonym: t3255

Alternate gene names: 29143588

Gene position: 3348008-3346518 (Counterclockwise)

Preceding gene: 29143589

Following gene: 29143587

Centisome position: 69.87

GC content: 54.93

Gene sequence:

>1491_bases
ATGAGTACTTCTACCCAGAACATCCCGTGGTATCGCCATCTCAACCGGGCGCAATGGCGGGCATTTTCCGCTGCCTGGTT
GGGATATCTGCTTGATGGTTTTGATTTTGTGTTAATTGCTCTTGTACTGACTGAGGTACAAAGCGAATTTGGGCTGACGA
CGGTACAGGCGGCAAGCCTGATTTCTGCGGCTTTTATCTCTCGCTGGTTCGGCGGGTTATTACTGGGCGCGATGGGCGAT
CGCTATGGGCGTCGTCTGGCGATGGTCAGCAGCATCATTCTGTTTTCGGTGGGAACCCTGGCCTGCGGGTTTGCGCCCGG
TTACACCACCATGTTCATCGCCCGACTGGTGATTGGTATGGGCATGGCGGGCGAATATGGTTCCAGCGCGACCTATGTGA
TTGAAAGCTGGCCAAAACATTTACGCAATAAAGCCAGTGGTTTTCTGATTTCCGGCTTCTCCGTCGGCGCGGTCGTTGCC
GCGCAGGTGTACAGCCTGGTGGTGCCTGTCTGGGGCTGGCGCGCGCTGTTTTTCATTGGCATTTTGCCAATTATCTTCGC
CCTCTGGCTGCGGAAAAACATTCCGGAAGCGGAAGACTGGAAAGAAAAACACGCGGGTAAAGCGCCGGTACGCACGATGG
TCGACATTCTTTATCGGGGCGAACATCGTATCATCAACATTTTAATGACCTTCGCCGCCGCCGCTGCGCTGTGGTTCTGT
TTTGCCGGTAACCTACAAAATGCTGCGATTGTGGCGGGGCTGGGACTACTGTGCGCGGTTATCTTTATCAGCTTTATGGT
GCAGAGCAGCGGTAAACGCTGGCCCACTGGCGTCATGCTGATGCTGGTGGTACTGTTTGCTTTCCTCTATTCTTGGCCGA
TTCAGGCGCTATTACCTACTTATCTGAAAACCGAGCTGGCCTACGATCCGCATACGGTGGCGAATGTCCTGTTCTTTAGC
GGATTTGGCGCGGCGGTTGGTTGTTGCGTAGGCGGTTTTCTTGGCGACTGGCTGGGAACGCGTAAAGCATATGTCTGTAG
CCTGCTGGCCTCGCAAATCCTCATTATTCCGGTCTTTGCGATTGGCGGCACAAACGTCTGGGTTCTGGGTCTGCTACTGT
TTTTCCAACAGATGTTGGGGCAGGGGATTGCCGGGATTCTGCCGAAACTGATCGGCGGTTACTTCGATACCGATCAGCGC
GCGGCGGGGCTGGGCTTTACTTATAACGTCGGCGCGCTCGGCGGCGCGCTGGCACCGATCCTGGGAGCGCTGATCGCTCA
ACGTCTGGATCTGGGCACTGCGCTGGCATCGCTCTCTTTCAGCCTGACGTTTGTCGTGATCCTGCTTATTGGGCTTGATA
TGCCGTCTCGCGTACAGCGTTGGCTACGTCCGGAAGCGTTACGCACCCATGATGCTATCGACGACAAAACGTTCAGCGGA
GCCGTACCGCTTGGCAGTGGTAAAGGTGCCTTTGTAAAAACGAAAAGTTAA

Upstream 100 bases:

>100_bases
GGCGCTACGCTTGCCCGGCCTACAACAGTGCGAACAGTAGGCCGGATAAGGCGGAGCCGCCATTCGGCATAACGACAACA
AAAATCGTCGGGAGAGTAAA

Downstream 100 bases:

>100_bases
TCCGCTTGCCCGGTTTTCAACCGGGCAATATTTGTAAGGGAGTAGTATGTCGTTACTTGAACAACTGGATAAAAATATTG
CCGCTTCTGGCGGCCTGATT

Product: sialic acid transporter

Products: Proton [Cytoplasm]; N-acetylneuraminate [Cytoplasm] [C]

Alternate protein names: Sialic acid permease 1 [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSTSTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQSEFGLTTVQAASLISAAFISRWFGGLLLGAMGD
RYGRRLAMVSSIILFSVGTLACGFAPGYTTMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILYRGEHRIINILMTFAAAAALWFC
FAGNLQNAAIVAGLGLLCAVIFISFMVQSSGKRWPTGVMLMLVVLFAFLYSWPIQALLPTYLKTELAYDPHTVANVLFFS
GFGAAVGCCVGGFLGDWLGTRKAYVCSLLASQILIIPVFAIGGTNVWVLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQR
AAGLGFTYNVGALGGALAPILGALIAQRLDLGTALASLSFSLTFVVILLIGLDMPSRVQRWLRPEALRTHDAIDDKTFSG
AVPLGSGKGAFVKTKS

Sequences:

>Translated_496_residues
MSTSTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQSEFGLTTVQAASLISAAFISRWFGGLLLGAMGD
RYGRRLAMVSSIILFSVGTLACGFAPGYTTMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILYRGEHRIINILMTFAAAAALWFC
FAGNLQNAAIVAGLGLLCAVIFISFMVQSSGKRWPTGVMLMLVVLFAFLYSWPIQALLPTYLKTELAYDPHTVANVLFFS
GFGAAVGCCVGGFLGDWLGTRKAYVCSLLASQILIIPVFAIGGTNVWVLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQR
AAGLGFTYNVGALGGALAPILGALIAQRLDLGTALASLSFSLTFVVILLIGLDMPSRVQRWLRPEALRTHDAIDDKTFSG
AVPLGSGKGAFVKTKS
>Mature_495_residues
STSTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQSEFGLTTVQAASLISAAFISRWFGGLLLGAMGDR
YGRRLAMVSSIILFSVGTLACGFAPGYTTMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVAA
QVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILYRGEHRIINILMTFAAAAALWFCF
AGNLQNAAIVAGLGLLCAVIFISFMVQSSGKRWPTGVMLMLVVLFAFLYSWPIQALLPTYLKTELAYDPHTVANVLFFSG
FGAAVGCCVGGFLGDWLGTRKAYVCSLLASQILIIPVFAIGGTNVWVLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQRA
AGLGFTYNVGALGGALAPILGALIAQRLDLGTALASLSFSLTFVVILLIGLDMPSRVQRWLRPEALRTHDAIDDKTFSGA
VPLGSGKGAFVKTKS

Specific function: May be a sugar-cation symporter involved in sialic acid uptake [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Sialic acid transporter (TC 2.A.1.12.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082231, Length=496, Percent_Identity=94.5564516129032, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI87082404, Length=452, Percent_Identity=35.1769911504425, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1790550, Length=470, Percent_Identity=23.8297872340426, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788292, Length=227, Percent_Identity=25.1101321585903, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788942, Length=296, Percent_Identity=27.3648648648649, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081723, Length=407, Percent_Identity=23.8329238329238, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789941, Length=488, Percent_Identity=24.3852459016393, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI32564663, Length=188, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320595, Length=207, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004742
- InterPro:   IPR005828 [H]

Pfam domain/function: PF07690 MFS_1; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 53634; Mature: 53503

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCEEECCC
>Mature Secondary Structure 
STSTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTEVQSEFGLTTVQAASL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH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CCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; N-acetylneuraminate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + N-acetylneuraminate [Periplasm] = Proton [Cytoplasm] + N-acetylneuraminate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]