Click here to switch to the map view.

The map label for this gene is fruA [H]

Identifier: 29143512

GI number: 29143512

Start: 3267064

End: 3268491

Strand: Direct

Name: fruA [H]

Synonym: t3175

Alternate gene names: 29143512

Gene position: 3267064-3268491 (Clockwise)

Preceding gene: 29143511

Following gene: 29143513

Centisome position: 68.18

GC content: 47.34

Gene sequence:

>1428_bases
ATGAAAAAGATCATTGCAGTGACCGGCTGCCCTACCGGTATTGCGCATACATTTATGGCTGAAGAAGCCTTAAAAAATGC
CGCGAAAAAATTATCTGTTGAAATAAAAGTTGAAACCAATGGTGCCAGCGGTGTTGAAAATGCCATTCAACCTGCTGATC
TGGTAGATATTGCCGGAGTCATTATTGCGGCGGATAAAGATGTCTTACCTGATCGCTTTAATGGTTTACCGGTTATTGAA
GTACCGGTAAAAGAAGCAATCCATCATCCTGCGGAGCTGATTAACAAAGTTATTAATGGTGAAGCCCCAATACGTAAAGG
CGAATCAACCACCAGCACGGAAATTATCGAAAAAGAATCACTGGGGCGACAAATTTATAAGCACCTGATGAGTGGTGTTT
CAAATATGCTACCTTTTGTAGTTGCCGGAGGCATATTAATTGCGGTGTCGCTTTTATGGGGGATTTATTCCGCTGATCCT
AATTCAGCTGAATACAATGCAACCGCCGCAATGCTAATGAAAATCGGTCAGCAAGCCTTTTCGATTATGGTTCCGGTCTT
TACCGCTTATATTGCATTCTCTATTTCTGGCAGACCAGGGATGGTGGCTGGGTTTGTCGGCGGCTTACTGGCAAATACTA
CCGGAGCAGGATTTCTCGGTGGGATAATTGCCGGATTCGCCGCCGGTTACCTGATGTTATGGGTAAAGAATCGACTCGAA
GGCTTACCGCGTCAGTATGAAGGCCTGAAATCGATTTTTATTATGCCGCTCATTGGCGTACTGGTTATTGGCGTGTTGAT
GTCGCTGCTTGGGCAACCTGTCGCAGCTATCAATAATAGCATGATGAACTGGCTGGCATCGCTACAGGAAGCGAATCCAA
TATTGCTGGGTATTGTGGTGGGTGCAATGTGCTCTTTTGACTTTGGCGGCCCGGTAAACAAAGCCGCTTATGTCACCGGC
ACGCTTTTACTGGGCCAGGGCAACTTCTACTTTATGGCTGGTGTGTCTGCCGCATGTATTACTCCACCACTGGTAATTGC
TCTGGCGACCACCTTCTTTCCAAAAGGTTTTAGCGAGGAAGAACGTGCCGCAGGCATGGTTAACTATATCCTCGGTTGTA
CACACATCACTGAAGGCGCGATTCCTTTCGCAGCGAAAGATCCTTTGCGTGTCATCCCGATGATGATGATTGCTTCCAGC
ATTTCCGCAGTACTTAGCTACAGCTTGCGTATTCAGGTTCCGGCTCCTCACGGCGGCTTCTTAATTTTACCTTTGGTGAG
TCAGCCGCTGGCCTGGGTGTTATGTATCCTTGCGGGATCTGCATGTGGTGCGATGATGCTTGGCCTATGGAGATTGTGGG
CTGTGCGCAAAAATTCAGTCAACACAACTCCTGTAGCAAAGGCTGGAGGTCAGAATGCAGCTCTGTGA

Upstream 100 bases:

>100_bases
CAAGTTCTGCCGGACTAGGCCAACTTGCGGCAATAGAAACCTTACTTTCGCAAATCCACGTCAGAAAGCTGTAGGTTAAA
TTACAATATAAGTTCTGCTT

Downstream 100 bases:

>100_bases
ACACGATATTTTTATCAGCGATGAGCGCCTCGATAAAGTCACCGCTCTGCACCGCGTGGTGGAAAAGTTATCTGCGGCTG
GCAATACCACGCCTGACTAT

Product: sugar phosphotransferase transporter subunit IIBC

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKKIIAVTGCPTGIAHTFMAEEALKNAAKKLSVEIKVETNGASGVENAIQPADLVDIAGVIIAADKDVLPDRFNGLPVIE
VPVKEAIHHPAELINKVINGEAPIRKGESTTSTEIIEKESLGRQIYKHLMSGVSNMLPFVVAGGILIAVSLLWGIYSADP
NSAEYNATAAMLMKIGQQAFSIMVPVFTAYIAFSISGRPGMVAGFVGGLLANTTGAGFLGGIIAGFAAGYLMLWVKNRLE
GLPRQYEGLKSIFIMPLIGVLVIGVLMSLLGQPVAAINNSMMNWLASLQEANPILLGIVVGAMCSFDFGGPVNKAAYVTG
TLLLGQGNFYFMAGVSAACITPPLVIALATTFFPKGFSEEERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIASS
ISAVLSYSLRIQVPAPHGGFLILPLVSQPLAWVLCILAGSACGAMMLGLWRLWAVRKNSVNTTPVAKAGGQNAAL

Sequences:

>Translated_475_residues
MKKIIAVTGCPTGIAHTFMAEEALKNAAKKLSVEIKVETNGASGVENAIQPADLVDIAGVIIAADKDVLPDRFNGLPVIE
VPVKEAIHHPAELINKVINGEAPIRKGESTTSTEIIEKESLGRQIYKHLMSGVSNMLPFVVAGGILIAVSLLWGIYSADP
NSAEYNATAAMLMKIGQQAFSIMVPVFTAYIAFSISGRPGMVAGFVGGLLANTTGAGFLGGIIAGFAAGYLMLWVKNRLE
GLPRQYEGLKSIFIMPLIGVLVIGVLMSLLGQPVAAINNSMMNWLASLQEANPILLGIVVGAMCSFDFGGPVNKAAYVTG
TLLLGQGNFYFMAGVSAACITPPLVIALATTFFPKGFSEEERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIASS
ISAVLSYSLRIQVPAPHGGFLILPLVSQPLAWVLCILAGSACGAMMLGLWRLWAVRKNSVNTTPVAKAGGQNAAL
>Mature_475_residues
MKKIIAVTGCPTGIAHTFMAEEALKNAAKKLSVEIKVETNGASGVENAIQPADLVDIAGVIIAADKDVLPDRFNGLPVIE
VPVKEAIHHPAELINKVINGEAPIRKGESTTSTEIIEKESLGRQIYKHLMSGVSNMLPFVVAGGILIAVSLLWGIYSADP
NSAEYNATAAMLMKIGQQAFSIMVPVFTAYIAFSISGRPGMVAGFVGGLLANTTGAGFLGGIIAGFAAGYLMLWVKNRLE
GLPRQYEGLKSIFIMPLIGVLVIGVLMSLLGQPVAAINNSMMNWLASLQEANPILLGIVVGAMCSFDFGGPVNKAAYVTG
TLLLGQGNFYFMAGVSAACITPPLVIALATTFFPKGFSEEERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIASS
ISAVLSYSLRIQVPAPHGGFLILPLVSQPLAWVLCILAGSACGAMMLGLWRLWAVRKNSVNTTPVAKAGGQNAAL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=445, Percent_Identity=42.9213483146067, Blast_Score=335, Evalue=5e-93,
Organism=Escherichia coli, GI1786951, Length=475, Percent_Identity=36.8421052631579, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1790386, Length=320, Percent_Identity=39.375, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI87082348, Length=481, Percent_Identity=32.2245322245322, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1788729, Length=387, Percent_Identity=25.8397932816537, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=40.8163265306122, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788730, Length=99, Percent_Identity=38.3838383838384, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49900; Mature: 49900

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIAVTGCPTGIAHTFMAEEALKNAAKKLSVEIKVETNGASGVENAIQPADLVDIAGV
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHCCCCHHHHHHHHE
IIAADKDVLPDRFNGLPVIEVPVKEAIHHPAELINKVINGEAPIRKGESTTSTEIIEKES
EEECCCCCCCHHCCCCCEEEECHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LGRQIYKHLMSGVSNMLPFVVAGGILIAVSLLWGIYSADPNSAEYNATAAMLMKIGQQAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SIMVPVFTAYIAFSISGRPGMVAGFVGGLLANTTGAGFLGGIIAGFAAGYLMLWVKNRLE
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLPRQYEGLKSIFIMPLIGVLVIGVLMSLLGQPVAAINNSMMNWLASLQEANPILLGIVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
GAMCSFDFGGPVNKAAYVTGTLLLGQGNFYFMAGVSAACITPPLVIALATTFFPKGFSEE
HHHHCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCHHHHCHHHHHHHHHHHCCCCCCHH
ERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIASSISAVLSYSLRIQVPAPHGGF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCE
LILPLVSQPLAWVLCILAGSACGAMMLGLWRLWAVRKNSVNTTPVAKAGGQNAAL
EEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIIAVTGCPTGIAHTFMAEEALKNAAKKLSVEIKVETNGASGVENAIQPADLVDIAGV
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHCCCCHHHHHHHHE
IIAADKDVLPDRFNGLPVIEVPVKEAIHHPAELINKVINGEAPIRKGESTTSTEIIEKES
EEECCCCCCCHHCCCCCEEEECHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
LGRQIYKHLMSGVSNMLPFVVAGGILIAVSLLWGIYSADPNSAEYNATAAMLMKIGQQAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SIMVPVFTAYIAFSISGRPGMVAGFVGGLLANTTGAGFLGGIIAGFAAGYLMLWVKNRLE
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLPRQYEGLKSIFIMPLIGVLVIGVLMSLLGQPVAAINNSMMNWLASLQEANPILLGIVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
GAMCSFDFGGPVNKAAYVTGTLLLGQGNFYFMAGVSAACITPPLVIALATTFFPKGFSEE
HHHHCCCCCCCCCCCCEEEEEEEEECCCEEEEECCCHHHHCHHHHHHHHHHHCCCCCCHH
ERAAGMVNYILGCTHITEGAIPFAAKDPLRVIPMMMIASSISAVLSYSLRIQVPAPHGGF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCE
LILPLVSQPLAWVLCILAGSACGAMMLGLWRLWAVRKNSVNTTPVAKAGGQNAAL
EEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]