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The map label for this gene is yflS [H]

Identifier: 29143428

GI number: 29143428

Start: 3167607

End: 3169061

Strand: Direct

Name: yflS [H]

Synonym: t3086

Alternate gene names: 29143428

Gene position: 3167607-3169061 (Clockwise)

Preceding gene: 29143427

Following gene: 29143429

Centisome position: 66.1

GC content: 55.95

Gene sequence:

>1455_bases
GTGGCGGAAAAAAAATCTCAGGGCGTCAAATGGCTCCCATTCATTCTTATTCTGGTTATCGCCGCCGGACTGTGGCAGCT
AACGCCCCCTTCCGGGTTAAGCGCGCCGGCCTGGCATTCCGCGATTATTTTTGTCGCGACGATCGCCTCTATCGTGGCGA
AAGTGCTGCCTATTGGCGCGGTCGGTATTATCGGCATCACCGTCTTTGCGCTCGCCTATGCTGCCGGCGACAAAACGGCC
AGCGGCGCGATTACCACGGCCTTGAGCGAGCTGAACAGTTCGCTGATCTGGCTTATCGTCGTCGCCTTTATGATTGCCCG
TGGGTTTATCAAAACCGGGCTGGGTCGGCGTATCGCCCTACAAATGATCCGTCTGTTAGGTAAACGAACGCTGGGTCTGG
CTTATGGCCTGGCGTTTGCCGATCTGATCCTCTCCCCCGCGATGCCCAGTAACACCGCGCGCTGCGGCGGCGTGATTTAC
CCGATCGCCGACTCGCTGGCGCGCAGCTTTGATTCACATCCGGAAGATGAGTCTCGCAGCAAAATCGGCACCTTTCTGAT
CACCTGTATTGGCAATGTTAACGACGTCACCGCCGCACTGTTTATGACCGGTTATACGGGTAACCTGCTGGCGGTAAAAC
TGGCGGCGAACGCCGGCGTTACGTTGAGCTGGGGAAGCTGGTTTATTGCCGCGCTCTTGCCTTGCCTGGTCTCTTTTCTT
ATCGTCCCGCTGCTGGTCTACTGGCTGACGCGTCCGGAAATTAAACATACGCCGGATGCGCCGGATCTGGCGCGCAAAGA
GCTGGCGCAAATGGGCAGTATGGCGCGCGGCGAGTGGCTCATGCTGGCGACCGTCGGTGTGTTGCTGGTGCTATGGATTT
TTGGCAGCTCGCTCGGCGTGGATGCCACGACGGCCTCTTTTGTCGGTCTCTCTATTTTATTACTGAGCGGCGTGCTCACC
TGGGAAGACGTTAAAAGCGAAAAAGGCGCCTGGGATACGTTGATTTGGTTTGCCGCGCTGCTGATGATGGCTAACCAGTT
GAAAAAACTAGGATTCACCAGTTGGTTTGGCAACCTGATTGGCGACAGCATCGGCAGTACGATGCATGGAACCAGTTGGA
TAATCATCCTGCTGCTGCTTAACGCAGCCTATTTTTATACCCACTATTTTTTCGCCAGCGGCAACGCGCAAATCGCCGCG
CTGTATGCGGTGTTTCTGGGTGTCGGCCTGCATCTGAATATACCGGCGGCGCCGATGGCGCTCATGCTGGCGTTTACCAG
CAGCCTGTACTGCTCTCTTACACAGTATACCCACGCACGCGGCCCCATTCTTTTTGGCGCCGGCTATGTACCGACGGGCA
TCTGGTGGCGAACGGGATTTATTATCAGCCTCTTTAACCAGGCGGTCTTTTTGACAGTCGGCCTCGCATGGTGGAAAGTG
TTAGGGTTGTATTAA

Upstream 100 bases:

>100_bases
CCCCACACACAAGCGCAGAATTATTCTGATTTTGTGAATATTTATTCACATTTATTATGAATTTACTTAATACATATAAA
ATATTTATGGATGTGTGATT

Downstream 100 bases:

>100_bases
ACGCTTAACGCATCGATAACCCCCTCGGTTAACGGAGAAATGGAATGACCTGCATTTTCTGTCAAATAGTTGAAGGTAAA
GCACCTTGTCATAAAGTTTG

Product: membrane transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MAEKKSQGVKWLPFILILVIAAGLWQLTPPSGLSAPAWHSAIIFVATIASIVAKVLPIGAVGIIGITVFALAYAAGDKTA
SGAITTALSELNSSLIWLIVVAFMIARGFIKTGLGRRIALQMIRLLGKRTLGLAYGLAFADLILSPAMPSNTARCGGVIY
PIADSLARSFDSHPEDESRSKIGTFLITCIGNVNDVTAALFMTGYTGNLLAVKLAANAGVTLSWGSWFIAALLPCLVSFL
IVPLLVYWLTRPEIKHTPDAPDLARKELAQMGSMARGEWLMLATVGVLLVLWIFGSSLGVDATTASFVGLSILLLSGVLT
WEDVKSEKGAWDTLIWFAALLMMANQLKKLGFTSWFGNLIGDSIGSTMHGTSWIIILLLLNAAYFYTHYFFASGNAQIAA
LYAVFLGVGLHLNIPAAPMALMLAFTSSLYCSLTQYTHARGPILFGAGYVPTGIWWRTGFIISLFNQAVFLTVGLAWWKV
LGLY

Sequences:

>Translated_484_residues
MAEKKSQGVKWLPFILILVIAAGLWQLTPPSGLSAPAWHSAIIFVATIASIVAKVLPIGAVGIIGITVFALAYAAGDKTA
SGAITTALSELNSSLIWLIVVAFMIARGFIKTGLGRRIALQMIRLLGKRTLGLAYGLAFADLILSPAMPSNTARCGGVIY
PIADSLARSFDSHPEDESRSKIGTFLITCIGNVNDVTAALFMTGYTGNLLAVKLAANAGVTLSWGSWFIAALLPCLVSFL
IVPLLVYWLTRPEIKHTPDAPDLARKELAQMGSMARGEWLMLATVGVLLVLWIFGSSLGVDATTASFVGLSILLLSGVLT
WEDVKSEKGAWDTLIWFAALLMMANQLKKLGFTSWFGNLIGDSIGSTMHGTSWIIILLLLNAAYFYTHYFFASGNAQIAA
LYAVFLGVGLHLNIPAAPMALMLAFTSSLYCSLTQYTHARGPILFGAGYVPTGIWWRTGFIISLFNQAVFLTVGLAWWKV
LGLY
>Mature_483_residues
AEKKSQGVKWLPFILILVIAAGLWQLTPPSGLSAPAWHSAIIFVATIASIVAKVLPIGAVGIIGITVFALAYAAGDKTAS
GAITTALSELNSSLIWLIVVAFMIARGFIKTGLGRRIALQMIRLLGKRTLGLAYGLAFADLILSPAMPSNTARCGGVIYP
IADSLARSFDSHPEDESRSKIGTFLITCIGNVNDVTAALFMTGYTGNLLAVKLAANAGVTLSWGSWFIAALLPCLVSFLI
VPLLVYWLTRPEIKHTPDAPDLARKELAQMGSMARGEWLMLATVGVLLVLWIFGSSLGVDATTASFVGLSILLLSGVLTW
EDVKSEKGAWDTLIWFAALLMMANQLKKLGFTSWFGNLIGDSIGSTMHGTSWIIILLLLNAAYFYTHYFFASGNAQIAAL
YAVFLGVGLHLNIPAAPMALMLAFTSSLYCSLTQYTHARGPILFGAGYVPTGIWWRTGFIISLFNQAVFLTVGLAWWKVL
GLY

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786986, Length=472, Percent_Identity=36.864406779661, Blast_Score=281, Evalue=1e-76,
Organism=Escherichia coli, GI1786829, Length=482, Percent_Identity=32.3651452282158, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789444, Length=486, Percent_Identity=35.1851851851852, Blast_Score=256, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 51963; Mature: 51832

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKKSQGVKWLPFILILVIAAGLWQLTPPSGLSAPAWHSAIIFVATIASIVAKVLPIGA
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
VGIIGITVFALAYAAGDKTASGAITTALSELNSSLIWLIVVAFMIARGFIKTGLGRRIAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
QMIRLLGKRTLGLAYGLAFADLILSPAMPSNTARCGGVIYPIADSLARSFDSHPEDESRS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCHHHHH
KIGTFLITCIGNVNDVTAALFMTGYTGNLLAVKLAANAGVTLSWGSWFIAALLPCLVSFL
HHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHH
IVPLLVYWLTRPEIKHTPDAPDLARKELAQMGSMARGEWLMLATVGVLLVLWIFGSSLGV
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DATTASFVGLSILLLSGVLTWEDVKSEKGAWDTLIWFAALLMMANQLKKLGFTSWFGNLI
CHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GDSIGSTMHGTSWIIILLLLNAAYFYTHYFFASGNAQIAALYAVFLGVGLHLNIPAAPMA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCEECCCHHHHH
LMLAFTSSLYCSLTQYTHARGPILFGAGYVPTGIWWRTGFIISLFNQAVFLTVGLAWWKV
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLY
HCCC
>Mature Secondary Structure 
AEKKSQGVKWLPFILILVIAAGLWQLTPPSGLSAPAWHSAIIFVATIASIVAKVLPIGA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
VGIIGITVFALAYAAGDKTASGAITTALSELNSSLIWLIVVAFMIARGFIKTGLGRRIAL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
QMIRLLGKRTLGLAYGLAFADLILSPAMPSNTARCGGVIYPIADSLARSFDSHPEDESRS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCHHHHH
KIGTFLITCIGNVNDVTAALFMTGYTGNLLAVKLAANAGVTLSWGSWFIAALLPCLVSFL
HHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHH
IVPLLVYWLTRPEIKHTPDAPDLARKELAQMGSMARGEWLMLATVGVLLVLWIFGSSLGV
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DATTASFVGLSILLLSGVLTWEDVKSEKGAWDTLIWFAALLMMANQLKKLGFTSWFGNLI
CHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GDSIGSTMHGTSWIIILLLLNAAYFYTHYFFASGNAQIAALYAVFLGVGLHLNIPAAPMA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCEECCCHHHHH
LMLAFTSSLYCSLTQYTHARGPILFGAGYVPTGIWWRTGFIISLFNQAVFLTVGLAWWKV
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLY
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]