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The map label for this gene is hybA [H]

Identifier: 29143414

GI number: 29143414

Start: 3153041

End: 3154027

Strand: Reverse

Name: hybA [H]

Synonym: t3070

Alternate gene names: 29143414

Gene position: 3154027-3153041 (Counterclockwise)

Preceding gene: 29143415

Following gene: 29143413

Centisome position: 65.82

GC content: 53.6

Gene sequence:

>987_bases
GTGAACAGACGTGACTTTATTAAAGCAGCCTCCGGTGGAGCATTGCTGTTGGGCGCCGCGCCGTCTGTCAGCCATGCGGC
TGCAGAAAACCGTCCGCCAATTCCAGGTTCGCTGGGCATGCTGTATGACTCCACTCTGTGCGTGGGCTGTCAGGCCTGCG
TCACAAAGTGTCAGGATATCAACTTCCCGGCGCGTAACCCGGAAGGGGAGCAGACCTGGTCGAACAACGACAAACTGTCG
CCGTATACCAATAACATTATCCAGGTATGGCGTAGCGGCACGGGCGTGAACAAAGATCAGGAAGAGAACGGCTATGCCTA
CATCAAGAAACAGTGTATGCACTGCGTCGATCCTAACTGCGTTTCCGTTTGTCCGGTGTCGGCGCTGAAAAAAGATCCGA
AAACCGGCATCGTCCACTATGACAAAGATGTCTGCACCGGCTGCCGTTACTGTATGGTCGCCTGTCCGTACAACGTACCT
AAGTACGACTACAACAACCCGTTTGGCGCGCTTCACAAGTGTGAGTTGTGTAACCAGAAAGGCGTTGAGCGTCTGGATAA
AGGCGGTTTGCCTGGGTGTGTGGAAGTTTGTCCGGCAGGGGCCGTCATTTTCGGTACTCGTGAAGAGCTAATGGCGGAAG
CGAAAAAACGTCTGGCCCTCAAACCAGGCAGTGAATATCACTATCCGCGCCAGACGTTGAAAACGGACGACACCTATCTG
CATACGGTGCCTAAATACTACCCACACCTCTACGGTGAAAAAGAGGGCGGTGGTACTCAGGTGCTGGTGCTGACAGGCGT
TCCTTATGAAGATTTGGATCTGCCGAAGCTGGATGACCTTTCAACCGGAGCGCGCTCCGAGCATGTTCAACACACGCTTT
ATAAAGGCATGATACTACCCCTGGCTGCGCTGGCGGGCTTAACCGTGCTGGTTCGTCGTAATAGCAAAAACGACCATCAC
GATGGAGGAGACGATCATGAGTCATGA

Upstream 100 bases:

>100_bases
GCGGCGTAGTCGGTCTGGTTGCCGGCGTCAGCGTGATGGCGGTACGTGAACTGGGGCGTCAGCAAAAGAAAGATAACGCT
GACTCACGGGGAGAATAACC

Downstream 100 bases:

>100_bases
TCCAAAACCGCTGGGCGGAAAAATCATCAGTAAACCGGTCATTATTTTTGGACCGTTAATCGTCCTGTGTATGCTCCTTA
TCGTGAAGCGTCTGGTCTTC

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNRRDFIKAASGGALLLGAAPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPARNPEGEQTWSNNDKLS
PYTNNIIQVWRSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKTDDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYEDLDLPKLDDLSTGARSEHVQHTLYKGMILPLAALAGLTVLVRRNSKNDHH
DGGDDHES

Sequences:

>Translated_328_residues
MNRRDFIKAASGGALLLGAAPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPARNPEGEQTWSNNDKLS
PYTNNIIQVWRSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKTDDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYEDLDLPKLDDLSTGARSEHVQHTLYKGMILPLAALAGLTVLVRRNSKNDHH
DGGDDHES
>Mature_328_residues
MNRRDFIKAASGGALLLGAAPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDINFPARNPEGEQTWSNNDKLS
PYTNNIIQVWRSGTGVNKDQEENGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKTDDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYEDLDLPKLDDLSTGARSEHVQHTLYKGMILPLAALAGLTVLVRRNSKNDHH
DGGDDHES

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=328, Percent_Identity=95.4268292682927, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI2367345, Length=255, Percent_Identity=30.9803921568627, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1787749, Length=283, Percent_Identity=28.6219081272085, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790326, Length=184, Percent_Identity=32.6086956521739, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787872, Length=196, Percent_Identity=35.2040816326531, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787122, Length=196, Percent_Identity=34.1836734693878, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510944, Length=207, Percent_Identity=31.8840579710145, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787478, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082180, Length=193, Percent_Identity=28.4974093264249, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789067, Length=197, Percent_Identity=28.9340101522843, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 35948; Mature: 35948

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRDFIKAASGGALLLGAAPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCHHHEEECCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPARNPEGEQTWSNNDKLSPYTNNIIQVWRSGTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCEEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKTDDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCHHH
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYEDLDLPKLDDLSTGARSEHVQHTLYKGMILP
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAALAGLTVLVRRNSKNDHHDGGDDHES
HHHHHHHEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRRDFIKAASGGALLLGAAPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
CCCHHHEEECCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NFPARNPEGEQTWSNNDKLSPYTNNIIQVWRSGTGVNKDQEENGYAYIKKQCMHCVDPNC
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
HHCCCHHHHHCCCCCCEEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKTDDTYL
HHHHHHCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCHHH
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYEDLDLPKLDDLSTGARSEHVQHTLYKGMILP
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAALAGLTVLVRRNSKNDHHDGGDDHES
HHHHHHHEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]