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The map label for this gene is hybC [H]

Identifier: 29143412

GI number: 29143412

Start: 3150173

End: 3151876

Strand: Reverse

Name: hybC [H]

Synonym: t3068

Alternate gene names: 29143412

Gene position: 3151876-3150173 (Counterclockwise)

Preceding gene: 29143413

Following gene: 29143411

Centisome position: 65.77

GC content: 54.87

Gene sequence:

>1704_bases
ATGAGCCAGAGAATTACTATTGATCCGGTAACCCGTATTGAGGGTCATTTACGCATTGACTGCGAAATTGAGAACGGCGT
CGTATCCAAAGCATGGGCGTCCGGCACCATGTGGCGCGGTATGGAAGAGATCGTGAAGAACCGCGATCCGCGCGATGCGT
GGATGATTGTGCAACGTATTTGCGGCGTTTGTACGACAACTCACGCGATCTCCTCCGTTCGTGCGGCGGAAAGCGCGCTG
AATATTGATGTTCCCGTCAACGCGCAGTATATCCGTAATATTATTCTGGCGGCGCATACCACCCATGACCACATTGTCCA
TTTCTACCAGCTCTCTGCGCTGGACTGGGTGGACATTACCTCTGCGCTGAAAGCCGATCCGGAAAAAGCTTCTGCGATGC
TGGATGGCGTGTCGAGCTGGCACCTGAACAGCGCGCAAGAGTTCACGAAAGTTCAGAACAAGATCAAGGATCTTGTCGCT
AGCGGTCAGTTAGGTATTTTCGCCAATGGCTACTGGGGGCACCCGGCAATGCAGTTACCGCCGGAGGTCAACCTGATTGC
GGTCGCCCACTATCTGCAGGCGCTGGAATGTCAGCGTGACGCTAACCGCGTGGTGGCGTTGCTGGGCGGTAAATCGCCGC
ATATCCAGAACCTGGCGGTCGGTGGGGTGGCGAACCCGATCAATCTCGACAGCATCGGTACGTTGAACCTTGAACGCCTG
ATGTACATCAAATCGTTTATCGATAAGCTGAGCGATTTTGTCGAGCAGGTGTATAAAGTGGATACCGCAGTCATCGCGGC
CTACTATCCGGAATGGCTGGAGCGCGGCAAAGGCGCGGTGAACTACCTGAGCGCGCCGGAATTCCCGACCGACGGTAAAA
ACGGCAGTTTCCTGTTCCCGGGCGGTTACATTACCAATGCCGATCTGTCGACATACCGTCCGATCACCTCTCACTCCGAT
GAATTCCTGATTAAAGGTATTCAGGAGAGCGCGAAGCACGCCTGGTATAAAGATGAAGCGCCGCAGGCGCCGTGGGAAGG
CACCACGATTCCGGCTTACGACGGTTGGTCGGATGACGGTAAATACTCATGGGTCAAGTCGCCGACATTCTACGGCAAAA
CCGTCGAAGTCGGGCCGCTGGCGAACATGCTATGTAAACTGGCCGCAGGTCGTGAATCCACGCAGACCAAGCTCAATGAA
ATCATTGCGCTTTATCAGAAGCTGACCGGCAAAACGCTGGAAATGGCGCAACTTCACTCTACGCTGGGTCGTATCATCGG
GCGTACCGTTCACTGTTGTGAACTGCAAAACGTATTGCAGGATCAATACAACGCGCTGATTGTAAATATCGGCAAAGGCG
ATTACACCACGTTTGTGAAGCCGAACATTCAGGCGACGGGCGAGTTCAAAGGCGTGGGCTTCCTGGAGGCGCCGCGCGGT
ATGCTGTCTCACTGGATGGTGATTAAAGACGGTATTATCAGTAACTATCAGGCAGTGGTGCCATCGACCTGGAACTCAGG
TCCGCGTAACTTCAACGATGACGTGGGGCCGTACGAACAGTCGCTGGTCGGTACGCCGATCGCCGATCCGGCGAAGCCGC
TGGAAGTGGTACGTACCATCCACTCCTTCGACCCTTGCATGGCGTGCGCGGTGCACGTGGTGGACGCCGATGGCAATGAA
GTGGTCTCGGTGAAGGTTTTGTAA

Upstream 100 bases:

>100_bases
TGGCTATTGAGATTTGTGCATACATCGTACTCATTCGTCTACTGCCGATACTTCCTCCTTTAAAACAAAACGATCATAAT
CGTCATGAGGCGAGCAAAGC

Downstream 100 bases:

>100_bases
TGCGGATATTAGTCTTAGGGGTCGGCAATATTTTGCTGACCGATGAAGCCATCGGGGTACGTATTGTTGAAGCGTTAGAG
CAACGATACACCCTGTCGGA

Product: hydrogenase 2 large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHAISSVRAAESAL
NIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVA
SGQLGIFANGYWGHPAMQLPPEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFPGGYITNADLSTYRPITSHSD
EFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNE
IIALYQKLTGKTLEMAQLHSTLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIQATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTIHSFDPCMACAVHVVDADGNE
VVSVKVL

Sequences:

>Translated_567_residues
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHAISSVRAAESAL
NIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVA
SGQLGIFANGYWGHPAMQLPPEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFPGGYITNADLSTYRPITSHSD
EFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNE
IIALYQKLTGKTLEMAQLHSTLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIQATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTIHSFDPCMACAVHVVDADGNE
VVSVKVL
>Mature_566_residues
SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRICGVCTTTHAISSVRAAESALN
IDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDITSALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVAS
GQLGIFANGYWGHPAMQLPPEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERLM
YIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFPGGYITNADLSTYRPITSHSDE
FLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNEI
IALYQKLTGKTLEMAQLHSTLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIQATGEFKGVGFLEAPRGM
LSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTIHSFDPCMACAVHVVDADGNEV
VSVKVL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=567, Percent_Identity=93.6507936507936, Blast_Score=1120, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=592, Percent_Identity=42.2297297297297, Blast_Score=476, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 62456; Mature: 62325

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CCCCEECCCHHHCCCEEEEEEEECCCEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTTHAISSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCHHHHEEEHHHCCCCHHHHH
SALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVASGQLGIFANGYWGHPAMQLP
HHHHCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCHHHHH
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEECC
GGYITNADLSTYRPITSHSDEFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDG
CCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNEIIALYQKLTGKTLEMAQLHS
CEEEECCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIQATGEFKGVGFLEAPRG
HHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHCCHH
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTI
HHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
HSFDPCMACAVHVVDADGNEVVSVKVL
HCCCHHHHHEEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
SQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CCCEECCCHHHCCCEEEEEEEECCCEEHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHH
CGVCTTTHAISSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
HHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCHHHHEEEHHHCCCCHHHHH
SALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVASGQLGIFANGYWGHPAMQLP
HHHHCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
PEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCHHHHH
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEECC
GGYITNADLSTYRPITSHSDEFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDG
CCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNEIIALYQKLTGKTLEMAQLHS
CEEEECCCCCCCCEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TLGRIIGRTVHCCELQNVLQDQYNALIVNIGKGDYTTFVKPNIQATGEFKGVGFLEAPRG
HHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHCCHH
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTI
HHHHHHHHHCCHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
HSFDPCMACAVHVVDADGNEVVSVKVL
HCCCHHHHHEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]