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The map label for this gene is gsp [H]

Identifier: 29143404

GI number: 29143404

Start: 3143733

End: 3145589

Strand: Reverse

Name: gsp [H]

Synonym: t3060

Alternate gene names: 29143404

Gene position: 3145589-3143733 (Counterclockwise)

Preceding gene: 29143406

Following gene: 29143403

Centisome position: 65.64

GC content: 53.31

Gene sequence:

>1857_bases
ATGAGCAAAGGAACCACCAGTCAGGATGCCCCCTTCGGGACATTATTGGGTTACGCCCCCGGCGGCGTAGCGATCTACTC
TTCAGATTACAATTCCCTCGATCCGTGGGACGACGACGACGCAGCTTTTCGTAGCTACATCGACGATGAATACATGGGGC
ATAAATGGCAGTGCGTGGAATTTGCCCGCCGTTTTCTCTTTCTGAATTATGGCGTTGTTTTTACTGACGTCGGCATGGCC
TGGGAAATTTTCTCGCTGCGTTTTCTGCGCGAGGTCGTGAATGACAATATTTTGCCTTTGCAGGCCTTCCCGAACGGTTC
GCCGCGCGCGCCGGAGGCCGGGGCGTTGCTCATCTGGCAAAAGGGCGGCGAGTTTAACGAAACCGGGCACGTCGCGATTA
TCACCCAACTCCTGGATAACAAAATTCGCATTGCTGAGCAAAACGTTATCCACACGCCATTGCCGCCAGGGCAGCAGTGG
ACTCGCGAACTGGAAATGGTCGTAGAAAACGGGTGTTACACCCTGCGCGACACCTTTGATGACACGACGATTTTAGGCTG
GATGATCCAGACCGACGACACGCAATACAGTTTGTCACAGCCTGACATCGCGAATCAGTCGCTGGCGATTCGCGGCGCAA
GGCTGCCAGAGAAAGGTCAGTTTGACGGTCAGTGGCTGGATGAGCGCGATCCGTTACAAAAAGCCTATGTACAGGCGAAC
GGACACGTTATCAATCAGGACCCGTACCAGTATTTTACCATCACCGAGAGCGCCGAACAGGAGCTTATCAAAGCGACCAA
TGAGTTGCATTTGATGTACCTGCACGCCACGGACAAAGTTTTAAAAGACGATAATCTGCTGGCGCTATTCGATATCCCCA
AAATTCTTTGGCCGCGCCTGCGTCTCTCCTGGCAGCGCCGTCGTCACCATATGATCACCGGTCGTATGGATTTCTGCATG
GATGAACGCGGGCTGAAAGTCTATGAATACAATGCCGATTCCGCATCATGCCATACCGAAGCGGGATTAATTCTCGAAAA
ATGGGCTGAGCAGGGCTATACCGGTAAAGGGCATAACCCGGCGGAGGGCTTAATTAACGAGCTGGCAGGCGCCTGGAAAC
ACAGTAAAGCGCGTCCTTTCGTCCATATCATGCAGGATGATGATATCGAGGAGGACTATCACGCGCAGTTTATGCAGCAG
GCGCTGCATCAGGCCGGTTTCGCGAGCAAAATTCTCCGCGGGCTGGGCGAGTTGCGCTGGGATGACGCCGGGCAGTTGAT
CGATGGCGACGGACGGCTGGTGAATTGTGTCTGGAAAACCTGGGCGTGGGAAACGGCAATGGAGCAGATACGCGAAGTCA
GCGAGACGGAGTATGCCGCAGTGCCTATTCGTACCGGGCACCCGGAAAATGAAGTCCGGCTGATTGATGTATTACTGCGC
CCGGAAGTGTTGGTGTTTGAACCGCTGTGGACGGTGATCCCGGGTAACAAAGCGATCCTGCCCATCCTGTGGTCGCTCTT
CCCACATCATCGCTATTTGCTCGACACGGATTTTACCGTTAACGATGAGCTGGTGCAGACCGGCTATGCGGTAAAACCGA
TTGCCGGACGTTGCGGTAGTAATATTGATTTGGTGAGCCATCAGGAAGAACTCCTGGATAAAACCAGCGGCAAGTTCGCC
ACGCAGAAAAATATCTATCAGCAGTTGTGGTGCCTGCCGAAGGTCGCCGGGAAGTATATTCAGGTCTGTACGTTTACCGT
GGGCGGTAACTACGGCGGTACGTGCCTGCGCGGCGATGATTCATTAGTCATTAAAAAAGAGAGCGATATTGAACCGTTAA
TCGTCATCAAAGCGTAA

Upstream 100 bases:

>100_bases
GTATGATTATGTCGACCTCTGTCCGGTTAGTGTCACAGAGCAGACAATCAGAGCGAAATCACGCCAGGCTGCTCAGGCGG
TCTTGTTTTGAGGTAAGTGC

Downstream 100 bases:

>100_bases
CGGGGTCATTCTCGTCGGGATATCGGCGTTGGCGCCGATATCCCGCCTGTTGTGTTTACCAACGAACGGCTGTCAGCTTG
CCATTTACTGGTAACATATG

Product: bifunctional glutathionylspermidine amidase/glutathionylspermidine synthetase

Products: NA

Alternate protein names: Glutathionylspermidine synthase; GSP synthetase; Glutathione:spermidine ligase [ADP-forming]; Glutathionylspermidine amidase; Glutathionylspermidine amidohydrolase [spermidine-forming]; GSP amidase [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYNSLDPWDDDDAAFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMA
WEIFSLRFLREVVNDNILPLQAFPNGSPRAPEAGALLIWQKGGEFNETGHVAIITQLLDNKIRIAEQNVIHTPLPPGQQW
TRELEMVVENGCYTLRDTFDDTTILGWMIQTDDTQYSLSQPDIANQSLAIRGARLPEKGQFDGQWLDERDPLQKAYVQAN
GHVINQDPYQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCM
DERGLKVYEYNADSASCHTEAGLILEKWAEQGYTGKGHNPAEGLINELAGAWKHSKARPFVHIMQDDDIEEDYHAQFMQQ
ALHQAGFASKILRGLGELRWDDAGQLIDGDGRLVNCVWKTWAWETAMEQIREVSETEYAAVPIRTGHPENEVRLIDVLLR
PEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVQTGYAVKPIAGRCGSNIDLVSHQEELLDKTSGKFA
TQKNIYQQLWCLPKVAGKYIQVCTFTVGGNYGGTCLRGDDSLVIKKESDIEPLIVIKA

Sequences:

>Translated_618_residues
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYNSLDPWDDDDAAFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMA
WEIFSLRFLREVVNDNILPLQAFPNGSPRAPEAGALLIWQKGGEFNETGHVAIITQLLDNKIRIAEQNVIHTPLPPGQQW
TRELEMVVENGCYTLRDTFDDTTILGWMIQTDDTQYSLSQPDIANQSLAIRGARLPEKGQFDGQWLDERDPLQKAYVQAN
GHVINQDPYQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCM
DERGLKVYEYNADSASCHTEAGLILEKWAEQGYTGKGHNPAEGLINELAGAWKHSKARPFVHIMQDDDIEEDYHAQFMQQ
ALHQAGFASKILRGLGELRWDDAGQLIDGDGRLVNCVWKTWAWETAMEQIREVSETEYAAVPIRTGHPENEVRLIDVLLR
PEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVQTGYAVKPIAGRCGSNIDLVSHQEELLDKTSGKFA
TQKNIYQQLWCLPKVAGKYIQVCTFTVGGNYGGTCLRGDDSLVIKKESDIEPLIVIKA
>Mature_617_residues
SKGTTSQDAPFGTLLGYAPGGVAIYSSDYNSLDPWDDDDAAFRSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAW
EIFSLRFLREVVNDNILPLQAFPNGSPRAPEAGALLIWQKGGEFNETGHVAIITQLLDNKIRIAEQNVIHTPLPPGQQWT
RELEMVVENGCYTLRDTFDDTTILGWMIQTDDTQYSLSQPDIANQSLAIRGARLPEKGQFDGQWLDERDPLQKAYVQANG
HVINQDPYQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMD
ERGLKVYEYNADSASCHTEAGLILEKWAEQGYTGKGHNPAEGLINELAGAWKHSKARPFVHIMQDDDIEEDYHAQFMQQA
LHQAGFASKILRGLGELRWDDAGQLIDGDGRLVNCVWKTWAWETAMEQIREVSETEYAAVPIRTGHPENEVRLIDVLLRP
EVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVQTGYAVKPIAGRCGSNIDLVSHQEELLDKTSGKFAT
QKNIYQQLWCLPKVAGKYIQVCTFTVGGNYGGTCLRGDDSLVIKKESDIEPLIVIKA

Specific function: Catalyzes the formation of an amide bond between glutathione and spermidine coupled with hydrolysis of ATP; also catalyzes the hydrolysis of glutathionylspermidine to glutathione and spermidine [H]

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 peptidase C51 domain [H]

Homologues:

Organism=Escherichia coli, GI1789361, Length=618, Percent_Identity=90.1294498381877, Blast_Score=1167, Evalue=0.0,
Organism=Escherichia coli, GI1789416, Length=395, Percent_Identity=26.5822784810127, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1790629, Length=384, Percent_Identity=26.5625, Blast_Score=122, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007921
- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF05257 CHAP; PF03738 GSP_synth [H]

EC number: =6.3.1.8; =3.5.1.78 [H]

Molecular weight: Translated: 70241; Mature: 70110

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50911 CHAP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYNSLDPWDDDDAAFRSYIDDEYMGHKWQCVE
CCCCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCCCHHHHHHHCCHHHCCCCHHHHH
FARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPEAGALLIWQ
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEE
KGGEFNETGHVAIITQLLDNKIRIAEQNVIHTPLPPGQQWTRELEMVVENGCYTLRDTFD
CCCCCCCCCCCEEEEHHHCCCEEEECCCEEECCCCCCHHHHHHHHHHHHCCCEEEECCCC
DTTILGWMIQTDDTQYSLSQPDIANQSLAIRGARLPEKGQFDGQWLDERDPLQKAYVQAN
CCEEEEEEEECCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
GHVINQDPYQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRL
CEEECCCCCEEEEECCHHHHHHHHCCCCEEEEEEECCCCEECCCCEEEEECCHHHHHHHH
RLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILEKWAEQGYTGKGHNP
HHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCH
AEGLINELAGAWKHSKARPFVHIMQDDDIEEDYHAQFMQQALHQAGFASKILRGLGELRW
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
DDAGQLIDGDGRLVNCVWKTWAWETAMEQIREVSETEYAAVPIRTGHPENEVRLIDVLLR
CCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEECC
PEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVQTGYAVKPIAGRCGS
CCEEEECCHHEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCCCHHCCCC
NIDLVSHQEELLDKTSGKFATQKNIYQQLWCLPKVAGKYIQVCTFTVGGNYGGTCLRGDD
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEECCC
SLVIKKESDIEPLIVIKA
CEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SKGTTSQDAPFGTLLGYAPGGVAIYSSDYNSLDPWDDDDAAFRSYIDDEYMGHKWQCVE
CCCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCCCCCHHHHHHHCCHHHCCCCHHHHH
FARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQAFPNGSPRAPEAGALLIWQ
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEE
KGGEFNETGHVAIITQLLDNKIRIAEQNVIHTPLPPGQQWTRELEMVVENGCYTLRDTFD
CCCCCCCCCCCEEEEHHHCCCEEEECCCEEECCCCCCHHHHHHHHHHHHCCCEEEECCCC
DTTILGWMIQTDDTQYSLSQPDIANQSLAIRGARLPEKGQFDGQWLDERDPLQKAYVQAN
CCEEEEEEEECCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
GHVINQDPYQYFTITESAEQELIKATNELHLMYLHATDKVLKDDNLLALFDIPKILWPRL
CEEECCCCCEEEEECCHHHHHHHHCCCCEEEEEEECCCCEECCCCEEEEECCHHHHHHHH
RLSWQRRRHHMITGRMDFCMDERGLKVYEYNADSASCHTEAGLILEKWAEQGYTGKGHNP
HHHHHHHHHHEEECCHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCH
AEGLINELAGAWKHSKARPFVHIMQDDDIEEDYHAQFMQQALHQAGFASKILRGLGELRW
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
DDAGQLIDGDGRLVNCVWKTWAWETAMEQIREVSETEYAAVPIRTGHPENEVRLIDVLLR
CCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEECC
PEVLVFEPLWTVIPGNKAILPILWSLFPHHRYLLDTDFTVNDELVQTGYAVKPIAGRCGS
CCEEEECCHHEECCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCCCCHHCCCC
NIDLVSHQEELLDKTSGKFATQKNIYQQLWCLPKVAGKYIQVCTFTVGGNYGGTCLRGDD
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEECCC
SLVIKKESDIEPLIVIKA
CEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7775463; 9278503 [H]