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The map label for this gene is iciA [H]

Identifier: 29143332

GI number: 29143332

Start: 3073609

End: 3074502

Strand: Direct

Name: iciA [H]

Synonym: t2982

Alternate gene names: 29143332

Gene position: 3073609-3074502 (Clockwise)

Preceding gene: 29143329

Following gene: 29143340

Centisome position: 64.14

GC content: 57.72

Gene sequence:

>894_bases
ATGAAACGTCCGGACTACAGAACACTACAGGCGCTGGATGCCGTTATACGTGAACGAGGATTCGAGCGCGCCGCCCAGAA
GCTGTGCATCACGCAATCCGCCGTCTCACAGCGCATAAAACAGCTTGAGAATATGTTCGGACAACCTTTGTTGGTGCGTA
CCGTTCCTCCTCGCCCCACGGAGCAAGGGCAAAAATTACTGGCTCTGCTGCGTCAGGTTGAACTGCTGGAAGAAGAGTGG
CTGGGCGATGAACAAACCGGCTCTACGCCGCTACTGCTGTCGCTGGCGGTTAACGCCGACAGTCTGGCGACCTGGCTGCT
TCCGGCGCTGGCGCCAGTACTGGCGGACTCCCCTATCCGTCTCAATTTACAGGTGGAAGATGAAACGCGCACGCAAGAAC
GTCTGCGTCGCGGCGAGGTGGTGGGCGCGGTGAGTATTCAGCATCAGGCGCTGCCCAGTTGCCTGGTAGACAAACTGGGC
GCGCTCGACTATCTGTTTGTCGCGTCAAAGCCGTTTGCCGAGCGCTATTTTCCCAATGGCGTTACCCGGTCGTCGCTGCT
GAAAGCGCCCGCCGTCGCGTTCGACCATCTGGATGATATGCATCAGGCTTTTTTGCAACAGAATTTCGATCTACCGCCCG
GCAGCGTGCCGTGCCATATCGTTAATTCATCCGAAGCGTTTGTACAGCTTGCGCGTCAGGGCACGGCCTGCTGCATGATC
CCTCACCTGCAGATTGAGAAAGAGCTGGAAAGCGGCGAGTTGATCAACTTAACGCCTGGCTTGCTACAACGCCGGATGCT
TTACTGGCACCGCTTTGCCCCGGAAAGCCGGATGATGCGCAAAGTGACCGACGCGCTGCTGGAGTACGGGCACAAGGTGC
TGCGTCAGGATTAG

Upstream 100 bases:

>100_bases
GGAAAAATCTCCGTGTCCGTACACAGGCTACGGATTTCATCTGTACCGACGGCAAATTTATGTCATAGTGCGGAAAAAAT
AACAGGAGCATGACACAACA

Downstream 100 bases:

>100_bases
CCTCTCTATGAATGCCGGATGGCGGCGTGAACGTCTTATCCGGCCTGCATGACCCATTATTCCCCCGTCGCTTCTTTGAA
ATTGATCACAAAATCATTGC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENMFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEW
LGDEQTGSTPLLLSLAVNADSLATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
ALDYLFVASKPFAERYFPNGVTRSSLLKAPAVAFDHLDDMHQAFLQQNFDLPPGSVPCHIVNSSEAFVQLARQGTACCMI
PHLQIEKELESGELINLTPGLLQRRMLYWHRFAPESRMMRKVTDALLEYGHKVLRQD

Sequences:

>Translated_297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENMFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEW
LGDEQTGSTPLLLSLAVNADSLATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
ALDYLFVASKPFAERYFPNGVTRSSLLKAPAVAFDHLDDMHQAFLQQNFDLPPGSVPCHIVNSSEAFVQLARQGTACCMI
PHLQIEKELESGELINLTPGLLQRRMLYWHRFAPESRMMRKVTDALLEYGHKVLRQD
>Mature_297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENMFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEW
LGDEQTGSTPLLLSLAVNADSLATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
ALDYLFVASKPFAERYFPNGVTRSSLLKAPAVAFDHLDDMHQAFLQQNFDLPPGSVPCHIVNSSEAFVQLARQGTACCMI
PHLQIEKELESGELINLTPGLLQRRMLYWHRFAPESRMMRKVTDALLEYGHKVLRQD

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=297, Percent_Identity=96.969696969697, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33479; Mature: 33479

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENMFGQPLLVRTVPPRPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSLATWLLPALAPVLADSPIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEE
LNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLGALDYLFVASKPFAERYFPNG
EEEEECCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHCCCC
VTRSSLLKAPAVAFDHLDDMHQAFLQQNFDLPPGSVPCHIVNSSEAFVQLARQGTACCMI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCEEEEE
PHLQIEKELESGELINLTPGLLQRRMLYWHRFAPESRMMRKVTDALLEYGHKVLRQD
CCHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENMFGQPLLVRTVPPRPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSLATWLLPALAPVLADSPIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEE
LNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLGALDYLFVASKPFAERYFPNG
EEEEECCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHCCCC
VTRSSLLKAPAVAFDHLDDMHQAFLQQNFDLPPGSVPCHIVNSSEAFVQLARQGTACCMI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHCCCEEEEE
PHLQIEKELESGELINLTPGLLQRRMLYWHRFAPESRMMRKVTDALLEYGHKVLRQD
CCHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA